Data/hora: 10/06/2024 07:58:59

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 24/08/2005
Data da última atualização: | 24/08/2005
Autoria: | SA, M. F. G. de; CHRISPEELS, M. J.
Título: | Molecular cloning of bruchid (Zabrotes subfasciatus) alpha-amylase cDNA and interactions of the expressed enzyme with bean amylase inhibitors.
Ano de publicação: | 1997
Fonte/Imprenta: | Insect Biochemistry and Molecular Biology, v. 27, n. 4, p. 271-281, 1997.
Idioma: | Inglês
Conteúdo: | Introduction; Materials and methods: Insects, RNA extraction, Northern blot analysis, Larval extraction, Reverse transcript-polymerase chain reaction and cDNA cloning, Cells plasmid, recombinant virus and insect cell transfection, Analysis of expressed Zs alfa-amylase and amylase activity detection, Protein assay, Binding ability of the inhibitor (alfaAI-2) to expressed Zs alfa-amylase; Results: Cloning of the ZsAmy cDNA, Analysis of the transcript, ZsAmy encodes and active amylase, Inhibition of Zs-amylase by bean alfa-amylase inhibitiors, at 30°C, ZsAmy forms a complex with alfaAI-2, but not with alfaAI-1. Discussion.
Thesagro: | Caruncho; Clonagem; Feijão; Phaseolus Vulgaris; Zabrotes Subfasciatus.
Categoria do assunto: | --
Registro original: | Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)
Exemplares: | CENARGEN - (25443-1) - UPC - AP - -- - 595.7005 -  -  - DIS