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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
04/01/1993 |
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Data da última atualização: |
04/01/1993 |
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Autoria: |
TSUKADA, M.; KUSANO, T.; KITAGAWA, Y. |
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Título: |
Introduction of foreign genes into tomato protoplasts by electroporation. |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Plant Cell Physiology, Kyoto, v.30, n.4, p.599-604, 1989. |
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Idioma: |
Inglês |
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Conteúdo: |
Optimum transfer of a plasmid harbouring the gene for chloramphenicol acetyltransferase (CAT) into protoplasts from cultivated tomato leaves was achieved by electroporation at 666 or 7000 V/cm (single discharge) using a 47 5F capacitor in the presence of 10-100 5g DNA. The CAT gene was strongly expressed when under the control of the CaMV 35S RNA promoter. CAT activity was detected in cells 10 days after electroporation. |
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Palavras-Chave: |
Eletroporacao; Promoter; Protoplastos; Technique; Tomato; Vegetable. |
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Thesagro: |
Biotecnologia; Engenharia Genética. |
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Thesaurus Nal: |
biotechnology; chloramphenicol acetyltransferase; electroporation; genetic transformation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01209naa a2200289 a 4500
001 1278661
005 1993-01-04
008 1989 bl --- 0-- u #d
100 1 $aTSUKADA, M.
245 $aIntroduction of foreign genes into tomato protoplasts by electroporation.
260 $c1989
520 $aOptimum transfer of a plasmid harbouring the gene for chloramphenicol acetyltransferase (CAT) into protoplasts from cultivated tomato leaves was achieved by electroporation at 666 or 7000 V/cm (single discharge) using a 47 5F capacitor in the presence of 10-100 5g DNA. The CAT gene was strongly expressed when under the control of the CaMV 35S RNA promoter. CAT activity was detected in cells 10 days after electroporation.
650 $abiotechnology
650 $achloramphenicol acetyltransferase
650 $aelectroporation
650 $agenetic transformation
650 $aBiotecnologia
650 $aEngenharia Genética
653 $aEletroporacao
653 $aPromoter
653 $aProtoplastos
653 $aTechnique
653 $aTomato
653 $aVegetable
700 1 $aKUSANO, T.
700 1 $aKITAGAWA, Y.
773 $tPlant Cell Physiology, Kyoto$gv.30, n.4, p.599-604, 1989.
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Registro original: |
Embrapa Florestas (CNPF) |
