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| Registros recuperados : 283 | |
| 241. |  | FIRMINO, A. A. P.; EVARISTO, R. G. S.; FRANCO, O. L.; CARNEIRO, R. M. D. G.; SILVEIRA, E. R.; SOUZA, D. S. L.; SILVA, L. P.; MAGALHÃES, B. S.; SILVA, M. C. M.; SOUSA, B. A.; SA, M. F. G. de; ROCHA, T. L. Identification of secondary metabolites from Canavalia ensiformis: perspectives for the use of metabolic engineering to control nematodes. In: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Resumos... Salvador: SBG, 2008. p. 281. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 242. | | SOUSA, B. A.; SILVA, O. N.; PORTO, W. F.; ROCHA, T. L.; SILVA, L. P. da; LEAL, A. P. F.; BUCCINI, D. F.; FAJEMIROYE, J. O.; CALDAS, R. de A.; FRANCO, O. L.; SA, M. F. G. de; NUNEZ, C. de la F.; MORENO, S. E. Identification of the active principle conferring anti-inflammatory and antinociceptive properties in bamboo plant. Molecules, v. 26, 3054, 2021. Na publicação: Luciano Paulino Silva; Maria Fátima Grossi-de-Sá.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 243. | | RIBEIRO, T. P.; LOURENCO, I. T.; MELO, B. P. de; MORGANTE, C. V.; SALLES FILHO, A.; LINS, C. B. J.; FERREIRA, G. F.; MELLO, G. N.; MACEDO, L. L. P. de; LUCENA, W. A.; SILVA, M. C. M. da; OLIVEIRA‑NETO, O. B.; SA, M. F. G. de. Improved cotton transformation protocol mediated by Agrobacterium and biolistic combined-methods. Planta, v. 254, 20, 2021.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 244. | | MOREIRA-PINTO, C. E.; COELHO, R. R.; LEITE, A. G. B.; SILVEIRA, D. A.; SOUZA, D. A.; LOPES, R. B.; MACEDO, L. L. P. de; SILVA, M. C. M. da; RIBEIRO, T. P.; MORGANTE, C. V.; ANTONINO, J. D.; SA, M. F. G. de. Increasing Anthonomus grandis susceptibility to Metarhizium anisopliae through RNAi-induced AgraRelish knockdown: a perspective to combine biocontrol and biotechnology. Pest Management Science, v. 77, n. 9, p. 4054-4063, 2021. Na publicação: Leonardo L P Macedo; Maria C M Silva; Maria F Grossi-de-Sa.,| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 245. | | RIBEIRO, T. P.; MARTINS-DE-SA, D.; MACEDO, L. L. P. de; LOURENCO, I. T.; RUFFO, G. C.; SOUSA, J. P. A.; SANTANA, J. M. do R.; OLIVEIRA-NETO, O. B.; MOURA, S. M.; SILVA, M. C. M. da; MORGANTE, C. V.; OLIVEIRA, N. G. de; BASSO, M. F.; SA, M. F. G. de. Cotton plants overexpressing the Bacillus thuringiensis Cry23Aa and Cry37Aa binary-like toxins exhibit high resistance to the cotton boll weevil (Anthonomus grandis). Plant Science, v. 344, 112079, 2024. Na publicação: Leonardo Lima Pepino Macedo; Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 246. | | MOURA, S. M. de; FREITAS, E. O.; RIBEIRO, T. P.; PAES-DE-MELO, B.; ARRAES, F. B. M.; MACEDO, L. L. P. de; PAIXÃO, J. F. R.; LOURENCO, I. T.; ARTICO, S.; VALENÇA, D. da C.; SILVA, M. C. M. da; OLIVEIRA, A. C. de; MARCIO ALVES-FERREIRA, M.; SA, M. F. G. de. Discovery and functional characterization of novel cotton promoters with potential application to pest control. Plant Cell Reports, v. 41, p. 1589-1601, 2022. Na publicação: Leonardo Lima Pepino Macedo; Isabela T. Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 248. | | BARBOSA, A. E. A. de D.; FRAGOSO, R. da R.; SOUZA, D. dos S. de L.; FREIRE, E.; OLIVEIRA NETO, O. B. de; VIANA, A. A. B.; TOGAWA, R. C.; GUIMARÃES, L. M.; MARTINS, N. F.; CIA, E.; FERNANDEZ, D.; LIMA, L. M. de; SILVA, M. C. M. da; ROCHA, T. L.; SA, M. F. G. de. Differentially expressed genes in cotton plant genotypes infected with Meloidogyne incognita. Plant Science, v. 177, p. 492-497, 2009.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 249. | | BARBOSA, A. E. A. de D.; FRAGOSO, R. da R.; SOUZA, D. dos S. de L.; FREIRE, E.; OLIVEIRA NETO, O. B. de; VIANA, A. A. B.; TOGAWA, R. C.; GUIMARÃES, L. M.; MARTINS, N. F.; CIA, E.; FERNANDEZ, D.; LIMA, L. M. de; SILVA, M. C. M. da; ROCHA, T. L.; SA, M. F. G. de. Differentially expressed genes in cotton plant genotypes infected with Meloidogyne incognita. Plant Science, v. 177, p. 492-497, 2009.| Biblioteca(s): Embrapa Cerrados. |
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| 250. | | SA, M. F. G. de; SOUZA, D. S. L.; SOUZA JUNIOR, J. D. A.; GROSSI DE SÁ, M.; FRAGOSO, R. R.; OLIVEIRA, G.; NAKASU, E.; CARNEIRO, R. M. D. G.; PINTO, E. R. de C.; ALMEIDA, ENGLER, J.; ENLER, G.; MARTINS DE SÁ, C.; ROCHA, T. L. Ectopic expression of a Meloidogyne incognita dorsal gland protein in tobacco accelerates the nematode's life cycle. In: INTERNATIONAL CONGRESS OF TROPICAL NEMATOLOGY, 2., 2009, Maceió. Abstracts... Maceió: ONTA: SBN, 2009. 1 CD-ROM| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 251. | | SOUZA, D. S. L.; ROCHA, T. L.; SOUZA JUNIOR, J. D. A.; PINTO, E. R. de C.; BARBOSA, A. E. A. D.; OLIVEIRA, G. R.; FRAGOSO, R. R.; NAKASU, E. Y. T.; SOUSA, B. A.; PIRES, N. F.; DUSI, D. M. de A.; CARNEIRO, R. M. D. G.; ALMEIDA ENGLER, J.; ENGLER, G.; SA, M. F. G. de. Ectopic expression of a meloidogyne incognita dorsal gland protein in tobacco accelerates the nematode`s life cycle. Tropical Plant Pathology, Brasília, DF, v. 34, p. S285, 2009. Suplemento. Edição dos Resumos do XLII do Congresso Brasileiro de Fitopatologia, Rio de Janeiro, RJ, 2009.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 252. | | MENDES, R. A. G.; BASSO, M. F.; AMORA, D. X.; SILVA, A. P.; PAES-DE-MELO, B.; TOGAWA, R. C.; FREIRE, E. V. S. A.; LISEI-DE-SA, M. E.; MACEDO, L. L. P. de; LOURENCO, I. T.; SA, M. F. G. de. In planta RNAi approach targeting three M. incognita effector genes disturbed the process of infection and reduced plant susceptibility. Experimental Parasitology, v. 238, 2022, 108246. Na publicação: Erika Valéria Saliba Albuquerque; Leonardo Lima Pepino Macedo; Isabela Tristan Lourenço-Tessutti; Maria Fatima Grossi-de-Sa.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 253. | | MOREIRA, V. J. V.; PINHEIRO, D. H.; LOURENCO, I. T.; BASSO, M. F.; LISEI-DE-SA, M. E.; SILVA, M. C. M. da; DANCHIN, E. G. J.; GUIMARAES, P. M.; GRYNBERG, P.; BRASILEIRO, A. C. M.; MACEDO, L. L. P. de; MORGANTE, C. V.; ENGLER, J. de A.; SA, M. F. G. de. In planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility. Journal of Pest Science, v. 97, p. 411-427, 2024.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 254. | | SILVA, P. P. da; SOARES, L.; COSTA, J. G. da; VIANA, L. da S.; ANDRADE, J. C. F. de; GONÇALVES, E. R.; SANTOS, J. M. dos; BARBOSA, G. V. de S.; NASCIMENTO, V. X.; TODARO, A. R.; RIFFEL, A.; SA, M. F. G. de; BARBORSA, M. H. P.; SANTANA, A. E. G.; RAMALHO NETO, C. E. Path analysis for selection of drought tolerant sugarcane genotypes through physiological components. Industrial Crops and Products, v. 37, p. 11-19, 2012.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Tabuleiros Costeiros. |
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| 255. | | BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M. da; SA, M. F. G. de; FREIRE, E. V. S. A. Molecular cloning and characterization of an a-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei. Gene, v. 553, n. 1, p. 7-16, Dec. 2014.| Biblioteca(s): Embrapa Cerrados. |
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| 256. | | MARRA, B. M.; SOUZA, D. S. L.; AGUIAR, J. N.; FIRMINO, A. A. P.; SARTO, R. P. D.; SILVA, F. B.; ALMEIDA, C. D. S.; CARES, J. E.; COUTINHO, M. V.; MARTINS DE SA, C.; FRANCO, O. L.; SA, M. F. G. de. Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots. Peptides, v. 30, p. 825-831, 2009.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 259. | | RIBEIRO, D. G.; MOTA, A. P. Z.; SANTOS, I. R.; ARRAES. F. B. M.; GRYNBERG, P.; FONTES, W.; CASTRO, M. de S.; SOUSA, M. V. de; LISEI-DE-SÁ, M. E.; SA, M. F. G. de; FRANCO, O. L.; REIS, A. M. dos. NBS-LRR-WRKY genes and protease inhibitors (PIs) seem essential for cowpea resistance to root-knot nematode. Journal of Proteomics, v. 261, 2022. 104575. Na publicação: Maria Fatima Grossi-de-Sá; Angela Mehta.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 260. | | PRADO, G. S.; BAMOGO, P. K. Z.; ABREU, J. A. C. de; GILLET, F. X.; SANTOS, V. O. dos; SILVA, M. C. M. da; BRIZARD, J. P.; BEMQUERER, M. P.; BANGRATZ, M.; BRUGIDOU, C.; SÉRÉMÉ, D.; SA, M. F. G. de; LACOMBE, S. Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil alfa-amylase. BMC Biotechnology, v. 19, n. 1, p.15, 2019. Na publicação: Maria Cristina Mattar Silva, Maria Fatima Grossi-de-Sa.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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Data corrente: |
07/07/2022 |
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Data da última atualização: |
08/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
TÁVORA, F. T. P. K.; DINIZ, F. de A. dos S.; RÊGO-MACHADO, C. de M.; FREITAS, N. C.; ARRAES, F. B. M.; ANDRADE, E. C. de; FURTADO, L. L.; OSIRO, K. O.; SOUSA, N. L. de; CARDOSO, T. B.; MERTZ-HENNING, L. M.; OLIVEIRA, P. A. de; FEINGOLD, S. E.; HUNTER, W. B.; SA, M. F. G. de; KOBAYASHI, A. K.; NEPOMUCENO, A. L.; SANTIAGO, T. R.; MOLINARI, H. B. C. |
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Afiliação: |
FABIANO TOUZDJIAN PINHEIRO KOHLRAUSCH TÁVORA, UNB; FRANCISCO DE ASSIS DOS SANTOS DINIZ, UNB; CAMILA DE MORAES RÊGO-MACHADO, UNB; NATÁLIA CHAGAS FREITAS; FABRÍCIO BARBOSA MONTEIRO ARRAES; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; LEILA LOURENÇO FURTADO, UNB; KAREN OFUJI OSIRO, UNB; NATÁLIA LIMA DE SOUSA, IPADS-Balcarce (UEDD INTA-CONICET), Argentina; THIAGO BÉRGAMO CARDOSO, SEMPRE AgTech; LILIANE MARCIA MERTZ HENNING, CNPSO; PATRICIA ABRAO DE OLIVEIRA MOLINARI, CNPAE; SÉRGIO ENRIQUE FEINGOLD, IPADS-Balcarce (UEDD INTA-CONICET), Argentina; WAYNE B. HUNTER, USDA-ARS, United States; MARIA FATIMA GROSSI DE SA, Cenargen; ADILSON KENJI KOBAYASHI, CNPAE; ALEXANDRE LIMA NEPOMUCENO, CNPSO; THAÍS RIBEIRO SANTIAGO, UNB; HUGO BRUNO CORREA MOLINARI, SEMPRE AgTech. |
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Título: |
CRISPR/Cas- and Topical RNAi-Based Technologies for crop management and improvement: reviewing the risk assessment and challenges towards a more sustainable agriculture. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Bioengineering and Biotechnology, v. 10, 2022. Article 913728. |
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DOI: |
https://doi.org/10.3389/fbioe.2022.913728 |
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Idioma: |
Inglês |
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Conteúdo: |
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe. MenosClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public pe... Mostrar Tudo |
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Palavras-Chave: |
Exogenous dsRNA; Genome editin; Genome editing; Offtargets; Public acceptance; Regulatory aspects. |
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Thesagro: |
Genoma. |
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Thesaurus NAL: |
Gene silencing; Nanotechnology; Toxicity. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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260 $c2022
520 $aClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe.
650 $aGene silencing
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700 1 $aDINIZ, F. de A. dos S.
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700 1 $aFREITAS, N. C.
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700 1 $aANDRADE, E. C. de
700 1 $aFURTADO, L. L.
700 1 $aOSIRO, K. O.
700 1 $aSOUSA, N. L. de
700 1 $aCARDOSO, T. B.
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700 1 $aFEINGOLD, S. E.
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700 1 $aKOBAYASHI, A. K.
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773 $tFrontiers in Bioengineering and Biotechnology$gv. 10, 2022. Article 913728.
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