Data/hora: 21/06/2024 00:27:30

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 18/06/2021
Data da última atualização: | 18/06/2021
Autoria: | MELO, B. P.; LOURENCO, I. T.; FRAGA, O. T.; PINHEIRO, L. B.; LINS, C. B. de J.; MORGANTE, C. V.; ENGLER, J. A.; REIS, P. A. B.; SA, M. F. G. de; FONTES, E. P. B.
Afiliação: | BRUNO PAES MELO, UFV; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; OTTO TEIXEIRA FRAGA, UFV; LUANNA BEZERRA PINHEIRO, UCB; CAMILA BARROZO DE JESUS LINS, INCT Plant Stress-Biotech; CAROLINA VIANNA MORGANTE, CPATSA; JANICE ALMEIDA ENGLER, INRAE, France; PEDRO AUGUSTO BRAGA REIS, UFV; MARIA FATIMA GROSSI DE SA, Cenargen; ELIZABETH PACHECO BATISTA FONTES, UFV.
Título: | Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses.
Ano de publicação: | 2021
Fonte/Imprenta: | Scientific Reports, v. 11, 11178, 2021.
DOI: | https://doi.org/10.1038/s41598-021-90767-6
Idioma: | Inglês
Notas: | Na publicação:  Isabela Tristan Lourenço-Tessutti; Maria Fátima Grossi-de-Sá.

Conteúdo:

NACs are plant-specifc transcription factors involved in controlling plant development, stress responses, and senescence. As senescence-associated genes (SAGs), NACs integrate age- and stress-dependent pathways that converge to programmed cell death (PCD). In Arabidopsis, NACSAGs belong to well-characterized regulatory networks, poorly understood in soybean. Here, we interrogated the soybean genome and provided a comprehensive analysis of senescence-associated Glycine max (Gm) NACs. To functionally examine GmNAC-SAGs, we selected GmNAC065, a putative ortholog of Arabidopsis ANAC083/VNI2 SAG, and the cell death-promoting GmNAC085, an ANAC072 SAG putative ortholog, for analyses. Expression analysis of GmNAC065 and GmNAC085 in soybean demonstrated (i) these cell death-promoting GmNACs display contrasting expression changes during age- and stress-induced senescence; (ii) they are co-expressed with functionally diferent gene sets involved in stress and PCD, and (iii) are diferentially induced by PCD inducers. Furthermore, we demonstrated GmNAC065 expression delays senescence in Arabidopsis, a phenotype associated with enhanced oxidative performance under multiple stresses, higher chlorophyll, carotenoid and sugar contents, and lower stress-induced PCD compared to wild-type. In contrast, GmNAC085 accelerated stress-induced senescence, causing enhanced chlorophyll loss, ROS accumulation and cell death, decreased antioxidative system expression and activity. Accordingly, GmNAC065 and GmNAC085 targeted functionally contrasting sets of downstream AtSAGs, further indicating that GmNAC85 and
GmNAC065 regulators function inversely in developmental and environmental PCD.

Palavras-Chave: | GmNAC065 expression; NAC SAGs.
Thesagro: | Genoma; Planta.
Thesaurus Nal: | Arabidopsis; Soybeans.

Categoria do assunto:

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G Melhoramento Genético

URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/223872/1/s41598-021-90767-6.pdf
Registro original: | Embrapa Semiárido (CPATSA)

Exemplares:

CENARGEN - (38561-1) - UPC - AP - DD -  -  -  - DIS
CPATSA - (59560-1) - UPC - AP - DD -  -  -  - DIS