|
|
Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
06/01/2016 |
Data da última atualização: |
20/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GUIMARÃES, A. L. S.; PEREIRA, S. A.; KUSSANO, N. R.; DODE, M. A. N. |
Afiliação: |
A. L. S. GUIMARÃES, UnB; S. A. PEREIRA, UnB; N. R. KUSSANO, UnB; MARGOT ALVES NUNES DODE, CENARGEN. |
Título: |
The effect of pre-maturation culture using phosphodiesterase type 3 inhibitor and insulin, transferrin and selenium on nuclear and cytoplasmic maturation of bovine oocytes. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Zygote, p. 1-11, 2015. |
DOI: |
10.1017/S0967199415000064 |
Idioma: |
Inglês |
Conteúdo: |
This study aims to evaluate if a pre-maturation culture (PMC) using cilostamide as a meiotic inhibitor in combination with insulin, transferrin and selenium (ITS) for 8 or 24 h increases in vitro embryo production. To evaluate the effects of PMC on embryo development, cleavage rate, blastocyst rate, embryo size and total cell number were determined. When cilostamide (20 M) was used in PMC for 8 or 24 h, 98% of oocytes were maintained in germinal vesicles. Although the majority of oocytes resumed meiosis after meiotic arrest, the cleavage and blastocyst rates were lower than the control (P < 0.05). When the cilostamide concentration was lowered (10 M) and oocytes were arrested for 8 h, embryo development was improved (P < 0.05) and was similar (P > 0.05) to the control. The deleterious effect of 20 M cilostamide treatment for 24 h on a PMC was confirmed by lower cumulus cell viability, determined by trypan blue staining, in that group compared with the other groups. A lower concentration (10 M) and shorter exposure time (8 h) minimized that effect but did not improve embryo production. More studies should be performed to determine the |
Palavras-Chave: |
Cilostamide; Cultura pré-maturação; Meiotic arrest; Oócitos bovinos. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182435/1/effect-of-prematuration-culture-using-phosphodiesterase-type-3-inhibitor-and-insulin-transferrin-and-selenium-on-nuclear-and-cytoplasmic-maturation-of-bovine-oocytes.pdf
|
Marc: |
LEADER 01878naa a2200217 a 4500 001 2033071 005 2023-03-20 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1017/S0967199415000064$2DOI 100 1 $aGUIMARÃES, A. L. S. 245 $aThe effect of pre-maturation culture using phosphodiesterase type 3 inhibitor and insulin, transferrin and selenium on nuclear and cytoplasmic maturation of bovine oocytes.$h[electronic resource] 260 $c2015 520 $aThis study aims to evaluate if a pre-maturation culture (PMC) using cilostamide as a meiotic inhibitor in combination with insulin, transferrin and selenium (ITS) for 8 or 24 h increases in vitro embryo production. To evaluate the effects of PMC on embryo development, cleavage rate, blastocyst rate, embryo size and total cell number were determined. When cilostamide (20 M) was used in PMC for 8 or 24 h, 98% of oocytes were maintained in germinal vesicles. Although the majority of oocytes resumed meiosis after meiotic arrest, the cleavage and blastocyst rates were lower than the control (P < 0.05). When the cilostamide concentration was lowered (10 M) and oocytes were arrested for 8 h, embryo development was improved (P < 0.05) and was similar (P > 0.05) to the control. The deleterious effect of 20 M cilostamide treatment for 24 h on a PMC was confirmed by lower cumulus cell viability, determined by trypan blue staining, in that group compared with the other groups. A lower concentration (10 M) and shorter exposure time (8 h) minimized that effect but did not improve embryo production. More studies should be performed to determine the 653 $aCilostamide 653 $aCultura pré-maturação 653 $aMeiotic arrest 653 $aOócitos bovinos 700 1 $aPEREIRA, S. A. 700 1 $aKUSSANO, N. R. 700 1 $aDODE, M. A. N. 773 $tZygote, p. 1-11, 2015.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Trigo. |
Data corrente: |
24/03/1999 |
Data da última atualização: |
09/09/2015 |
Autoria: |
ALMEIDA, J. L.; ARIAS, G.; BLUM, M. M. C.; MINELLA, E.; RUPPEL, E. C.; SÓ e SILVA, M. |
Afiliação: |
1, 5 FAPA. Vitoria-Entre Rios, Guarapuava; 2, 4, 6, Embrapa Trigo; 3 Cooperativa Agrária Mista Entre Rios Ltda. Vitoria-Entre Rios, Guarapuava. |
Título: |
Bloco de cruzamento, Entre Rios - 1997. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
In: REUNIÃO ANUAL DE PESQUISA DE CEVADA, 18., 1998, Passo Fundo. Anais. Passo Fundo: EMBRAPA-CNPT, 1998. |
Páginas: |
p. 102-106 |
Idioma: |
Português |
Thesaurus NAL: |
barley. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00549naa a2200193 a 4500 001 1824983 005 2015-09-09 008 1998 bl uuuu u00u1 u #d 100 1 $aALMEIDA, J. L. 245 $aBloco de cruzamento, Entre Rios - 1997. 260 $c1998 300 $ap. 102-106 650 $abarley 700 1 $aARIAS, G. 700 1 $aBLUM, M. M. C. 700 1 $aMINELLA, E. 700 1 $aRUPPEL, E. C. 700 1 $aSÓ e SILVA, M. 773 $tIn: REUNIÃO ANUAL DE PESQUISA DE CEVADA, 18., 1998, Passo Fundo. Anais. Passo Fundo: EMBRAPA-CNPT, 1998.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Trigo (CNPT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|