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Registros recuperados : 128 | |
21. | | MENEZES JÚNIOR, I. de A.; OSEI, O.; MATOS, G. F. de; ROUWS, L. F. M. (Brady)rizóbios associadas à cana-de-açúcar: abundância, diversidade e o efeito do genótipo da planta In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., FERTBIO 2016. Goiânia. Rumo aos novos desafios: anais. Goiânia: UFG, 2016. Biblioteca(s): Embrapa Agrobiologia. |
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22. | | ROUWS, L. F. M.; MENESES, C. H. G.; GUEDES. H. V.; VIDAL, M. S.; SCHWAB, S. Use of reporter genes to monitor interactions between the diazotrophic bacterium Gluconacetobacter diazotrophicus and its host plants sugarcane and rice. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR SBBq, 39.; LATIN AMERICAN SYMPOSIUM ON THE MOLECULAR MECHANISMS OF SKELETAL MINERALIZATION, 1., 2010, Foz do Iguaçu, PR. Resumos... São Paulo: SBBq, 2010. Biblioteca(s): Embrapa Agrobiologia. |
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31. | | ROUWS, L. F. M.; MENESES, C. H. S. G.; VIDAL, M. S.; BALDANI, J. I. Evidence for an important role of the Gluconacetobacter diazotrophics Flp pilus during rice root surface colonization. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SVI.08 Biblioteca(s): Embrapa Agrobiologia. |
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32. | | SOUZA, M. L. da C.; SCHWAB, S.; BALDANI, J. I.; ROUWS, L. F. M. Marcação de Bradyrhizobium spp. com proteína verde fluorescente. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 19, 2019, Seropédica, RJ. Bioeconomia, diversidade e riqueza para o desenvolvimento sustentável. Caderno de resumos... Seropédica: Embrapa Agrobiologia, 2019. Biblioteca(s): Embrapa Agrobiologia. |
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33. | | GUEDES, H. V.; ROUWS, L. F. M.; SCHWAB, S.; SIMÕES-ARAÚJO, J. L.; BALDANI, J. I. A novel histidine kinase of Gluconacetobacter diazotrophicus might be involved in nitrogen fixation. In: INTERNATIONAL SYMPOSIUM ON NITROGEN FIXATION WITH NON-LEGUMES, 11th., 2008, Gent, Belgium. Abstracts... Belgium: Universiteit Gent, 2008. p. 9. Resumo PS1-5. Parceria: UFRRJ. Biblioteca(s): Embrapa Agrobiologia. |
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35. | | HOFMANN, A.; FISHER, D.; ROUWS, L. F. M.; SCHWAB, S.; HARTMANN, A.; BALDANI, J. I. Relevance of the Gluconacetobacter diazotrophicus PAL5 LUX/LUSR quorum sensing system for its plants growth promotion and plant microbe interaction. In: TALLER LATIONOAMERICANO DE PGPR, 3., 2016. Chile. Libro de Resúmenes. Pucón, CH: Universidad de La Frontera, 2016 Biblioteca(s): Embrapa Agrobiologia. |
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37. | | MENEZES, C.; GONÇALVES, T.; ALQUÉRES, S.; ROUWS, L. F. M.; SERRATO, R.; VIDAL, M. S.; BALDANI, J. I. Gluconacetobacter diazotrophicus exopolysaccharide protects bacterial cells against oxidative stress in vitro and during rice plant colonization Plant and Soil, v. 416, n. 1–2, p. 133–147, 2017. Biblioteca(s): Embrapa Agrobiologia. |
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39. | | MENESES, C. H. S. G.; ROUWS, L. F. M.; SIMÕES-ARAÚJO, J. L.; VIDAL, M. S.; BALDANI, J. I. O gene gumD de Gluconacetobacter diazotrophicus (PAL5T) é essencial para colonização em plântulas de arroz. In: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009, Seropédica. Ciência no Brasil: desafios, avanços e aplicações. Seropédica: Embrapa Agrobiologia, 2009. Biblioteca(s): Embrapa Agrobiologia. |
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40. | | MENESES, C. H. S. G.; ROUWS, L. F. M.; ARAUJO, J. L. S. de; VIDAL, M. S.; BALDANI, J. I. GumD gene involved in the initial steps of rice root colonization by Gluconacetobaceter diazotrophicus PAL5. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SlV.19 Biblioteca(s): Embrapa Agrobiologia. |
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Registros recuperados : 128 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
22/04/2008 |
Data da última atualização: |
16/03/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
ROUWS, L. F. M.; SIMÕES ARAÚJO, J. L.; HEMERLY, A. S.; BALDANI, J. I. |
Afiliação: |
Luc F. M. Rouws, UFRRJ; Jean Luis Simões Araújo, Embrapa Agrobiologia; Adriana S. Hemerly, Instituto de Pesquisas do Jardim Botânico do RJ; José Ivo Baladani, Embrapa Agrobiologia. |
Título: |
Validation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Archives of Microbiology, New York, v. 189, n. 4, p. 397-405, apr. 2008. |
Idioma: |
Inglês |
Notas: |
Parceria: UFRRJ; Instituto de Pesquisas do Jardim Botânico do Rio de Janeiro. |
Conteúdo: |
Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5?Tnp Transposome? (Epicentre). Up to 1 × 106 mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool. |
Palavras-Chave: |
Bactéria endofítica. |
Thesaurus NAL: |
Gluconacetobacter diazotrophicus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01936naa a2200193 a 4500 001 1626792 005 2015-03-16 008 2008 bl uuuu u00u1 u #d 100 1 $aROUWS, L. F. M. 245 $aValidation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant. 260 $c2008 500 $aParceria: UFRRJ; Instituto de Pesquisas do Jardim Botânico do Rio de Janeiro. 520 $aGluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5?<KAN-2>Tnp Transposome? (Epicentre). Up to 1 × 106 mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool. 650 $aGluconacetobacter diazotrophicus 653 $aBactéria endofítica 700 1 $aSIMÕES ARAÚJO, J. L. 700 1 $aHEMERLY, A. S. 700 1 $aBALDANI, J. I. 773 $tArchives of Microbiology, New York$gv. 189, n. 4, p. 397-405, apr. 2008.
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