Data/hora: 24/06/2024 05:37:41

Biblioteca(s): | Embrapa Florestas.
Data corrente: | 25/09/2008
Data da última atualização: | 25/09/2008
Autoria: | DECAENS, T.; ROUGERIE, R.; RICHARD, B.; JAMES, S.; HEBERT, P.
Título: | A taxonomic survey of Upper-Normandy earthorms with DNA barecodes.
Ano de publicação: | 2008
Fonte/Imprenta: | In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
Idioma: | Inglês

Conteúdo:

Earthworms are represented in most terrestrial ecosystems, where they perform key ecological
functions. Their relatively large body-size facilitates their sampling and their study when compared
with smaller groups of the soil fauna. Despite these characteristics, taxonomic knowledge of
these organisms is still weak, even for the family Lumbricidae which is the exclusive representative
of the group in Western Europe. Identification difficulties are particularly dramatic for juveniles
for which the classical diagnostic characters (structure and position of the clitellum) are absent.
The main objective of this study was to survey the taxonomy of earthworm species in Upper
Normandy by building a database of DNA barcodes (partial sequences of the mitochondrial
gene COI). In April 2007 and 2008, we made an exhaustive collection of individuals (about 20
per species) of the species pool of the Upper Normandy region (ca. 20 species). Special attention
was paid to inclusion of intraspecific phenotypic variability, by sampling specimens varying in
size and coloration, two characters usually neglected in earthworm taxonomy though they have
been shown to be potential indicators of cryptic diversity. Small fragments of cutaneous tissues
were sampled from each specimen before its fixation in formaldehyde and storage in alcohol.
Sequencing of the COI gene was performed at the Canadian Centre for DNA Barcoding, and
results were analysed through the BOLD bioinformatics platform (Ratnasingham & Hebert 2007).
The results allow exploration of some more specific aspects of earthworm taxonomy, such as
the diversity of local species that are known as invasive in other regions; examining some
taxonomic problems raised by polymorphic species suspected to be complex of cryptic species;
verifying the status of potential cryptic species revealed by DNA barcoding. Preliminary results
indicate that three of the most common species in the target area (Lumbricus terrestris,
Allolobophora chlorotica and Aporrectodea caliginosa) all represent complexes of cryptic
species.

Categoria do assunto: | --
Registro original: | Embrapa Florestas (CNPF)
Exemplares: | CNPF - (43713-1) - UPE - PL - -- - CD0305 -  -  - DIS