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Registros recuperados : 15 | |
3. | | ROSA, P. M. da; ANTONIASSI, R.; GONÇALVES, E. B.; BIZZO, H. R.; SILVA, A. J. R. da. Extração de ácido clorogênico de farelo de girassol desengordurado. Ciência Rural, Santa Maria, v. 41, n. 4, p. 719-724, abr. 2011. Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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5. | | DIAS, L. P.; ROSA, P. M. da S.; CAMARGO, A. J. dos R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A.; OLIVEIRA, C. S. Dinâmica folicular em doadoras de oócito das raças Gir e Holandesa mantidas em condições tropicais. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). 2 p. Biblioteca(s): Embrapa Gado de Leite. |
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6. | | ROSA, P. M. Da S.; BARROS, B. A. F. De; PASOLINI, R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Hormonal ovulation induction in prepubertal GYR heifers In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tours: Society for Reproductive Biology, 2016. p. 516-517. Biblioteca(s): Embrapa Gado de Leite. |
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7. | | ROSA, P. M. da S.; CAMARGO, A. J. dos R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A.; OLIVEIRA, C. S. Eficiência reprodutiva de novilhas pré-púberes da raça Gir na produção in vitro de embriões. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). 2 p. Biblioteca(s): Embrapa Gado de Leite. |
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8. | | ROSA, P. M. da S.; CAMARGO, A. J. DOS R.; SERAPIÃO, R, V.; ALVES, B. R. C.; NOGUEIRA, G. DE. P.; OLIVEIRA, C. S. Perfil de secreção de hormônio luteinizante em novilhas pré-púberes da raça Gir, submetidas a protocolo de indução de ovulação. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 20. 2017, 4 p. Anais... Juiz de Fora/MG. Embrapa Gado de Leite. Biblioteca(s): Embrapa Gado de Leite. |
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9. | | OLIVEIRA, C. S.; ROSA, P. M. da S.; CAMARGO, A. J. dos R.; FERES, L. F.; SARAIVA, N. Z.; OLIVEIRA, L. Z.; CAMARGO, L. S. de A. Outstanding Gir oocyte donors: how does individual factor affect in vitro embryo production efficiency? Animal Science Journal, v. 94, e13862, 2023. Biblioteca(s): Embrapa Gado de Leite. |
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10. | | ROSA, P. M. Da S.; BARROS, B. A. F. De; PASSOLINI, R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Caracterização de oócitos de animais pré-púberes da raga Gir: tamnho do oócito, morfologia e atividade metabólica. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. Biblioteca(s): Embrapa Gado de Leite. |
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11. | | ROSA, P. M. da S.; BARROS, B. A. F. de; PASOLINI, R.; CAMARGO, A. J. dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Indução de ovulação em novilhas pré-púberes da raça Gir. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 17., 2016, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2016. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 186). Biblioteca(s): Embrapa Gado de Leite. |
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13. | | FREITAS, C. de; QUINTAO, C. C. R.; BARROS, B. A. F. De; CAMARGO, A. J. Dos R.; ROSA, P. M. Da S.; SERAPIÃO, R. V.; CAMARGO, L. S. de A.; OLIVEIRA, C. S.; MONTEIRO, C. A. S. Desenvolvimento pós-implantacional de embriões holandês-gir biopsiados. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. 2 Biblioteca(s): Embrapa Gado de Leite. |
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14. | | OLIVEIRA, C. S.; ROSA, P. M. da S.; SARAIVA, H. F. R. de A.; FREITAS, C. de; CAMARGO, A. J. dos R.; QUINTAO, C. C. R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A. Influência do estádio de desenvolvimento na viabilidade de embriões bovinos Gir/Holandês submetidos à biópsia embrionária para genotipagem. In: CONGRESSO INTERNACIONAL DO LEITE, 13., 2015, Porto Alegre. Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 4 p. Biblioteca(s): Embrapa Gado de Leite. |
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15. | | OLIVEIRA, C. S.; ROSA, P. M. da S.; ALVES, B. R. C.; MONTEIRO, C. A. S.; LEAL, G. R.; GUEDES, P. H. E.; CAMARGO, A. J. dos R.; SARAIVA, N. Z. Luteinizing hormone secretion, ovulatory capacity, and oocyte quality in peripubertal Gir heifers. Zygote, v. 31, n. 4, p. 342-349, 2023. Biblioteca(s): Embrapa Gado de Leite. |
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Registros recuperados : 15 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
28/12/2023 |
Data da última atualização: |
22/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 3 |
Autoria: |
ROSA, P. M. da S.; GUEDES, P. H. E.; GARCIA, J. M.; OLIVEIRA, C. S. |
Afiliação: |
PAOLA MARIA DA SILVA ROSA, UNIVERSIDADE ESTADUAL PAULISTA; PEDRO HENRIQUE EVAGELISTA GUEDES; JOAQUIM MANSANO GARCIA, UNIVERSIDADE ESTADUAL PAULISTA; CLARA SLADE OLIVEIRA, CNPGL. |
Título: |
Cytoplasmic granules in bovine oocytes do not affect embryonic or fetal development. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Zygote, v. 32, n. 1, p. 28-37, 2023. |
DOI: |
https://doi.org/10.1017/S0967199423000576 |
Idioma: |
Inglês |
Conteúdo: |
Oocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytoplasm present equivalent efficiency for IVF and calf production compared with homogenous cytoplasm oocytes. This could be observed through similar cleavage, blastocyst rates, and fetal growth development. In addition to differences in oocyte gene expression related to oocyte quality, it seems not to affect oocyte developmental competence. MenosOocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytopla... Mostrar Tudo |
Palavras-Chave: |
Classificação morfológica; IVF; Oócito; Ooplasma; Ovócito. |
Thesagro: |
Bovino; Citoplasma; Embrião Animal; Granulo. |
Thesaurus NAL: |
Cytoplasm; Granules. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02681naa a2200301 a 4500 001 2160283 005 2024-01-22 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1017/S0967199423000576$2DOI 100 1 $aROSA, P. M. da S. 245 $aCytoplasmic granules in bovine oocytes do not affect embryonic or fetal development.$h[electronic resource] 260 $c2023 520 $aOocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytoplasm present equivalent efficiency for IVF and calf production compared with homogenous cytoplasm oocytes. This could be observed through similar cleavage, blastocyst rates, and fetal growth development. In addition to differences in oocyte gene expression related to oocyte quality, it seems not to affect oocyte developmental competence. 650 $aCytoplasm 650 $aGranules 650 $aBovino 650 $aCitoplasma 650 $aEmbrião Animal 650 $aGranulo 653 $aClassificação morfológica 653 $aIVF 653 $aOócito 653 $aOoplasma 653 $aOvócito 700 1 $aGUEDES, P. H. E. 700 1 $aGARCIA, J. M. 700 1 $aOLIVEIRA, C. S. 773 $tZygote$gv. 32, n. 1, p. 28-37, 2023.
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