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| 5. |  | SÁ JUNIOR, J. P. M. e; UCHÔA, B. F.; SKOGLEY, E. O. Subidios à caracterização de solos carentes em P e K para o milho na região fisiográfiaca do Nordeste do Brasil. Pesquisa Agropecuária Brasileira, Rio de Janeiro, v. 5, p. 351-357, 1970. (Agronomia, 3). Título em inglês: Additions to the characterization and mapping of soils deficient in Phosphorus and potassium, for corn, in the physiographic region of Northeast Brazil.
A partir do v. 3(1968) é constituída de duas partes: Série...| Biblioteca(s): Embrapa Unidades Centrais. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
29/05/2013 |
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Data da última atualização: |
24/06/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ROSA, A. J. de M.; SARDINA, M. T.; MASTRANGELO, S.; TOLONE, M.; PORTOLANO, B. |
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Afiliação: |
ARTUR JORDAO DE MAGALHAES ROSA, CPAC; M. T. SARDINA, UNIVERSITÀ DEGLI STUDI DI PALERMO; S. MASTRANGELO, UNIVERSITÀ DEGLI STUDI DI PALERMO; M. TOLONE, UNIVERSITÀ DEGLI STUDI DI PALERMO; B. PORTOLANO, UNIVERSITÀ DEGLI STUDI DI PALERMO. |
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Título: |
Parentage verification of Valle del Belice dairy sheep using multiplex microsatellite panel. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 113, n. 1, p. 62-65, June 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this work was to develop and evaluate a PCR based microsatellite markers multiplex system for parentage verification of Sicilian Valle del Belice dairy sheep. A total of 85 samples of blood and hair were collected and genotyped for 24 microsatellite markers in multiplex electrophoresis runs. A total of 269 alleles were detected across the 24 loci investigated. The PIC considering all loci was equal to 0.736, showing that this microsatellite panel was very polymorphic and highly informative. A parentage test was performed on 64 families generated with multiple sires. Results indicated 20.3% and 29.7% misidentification rates for females and males, respectively. In 8 cases, out of 13 maternal exclusions, the real mother was identified among other females within the flock. The observed misidentification rates indicated the necessity of keeping more efficient collection of genealogical records, in order to properly control inbreeding or implement a breeding program. The parentage test presented here could be a helpful tool on verifying or even reconstructing the current pedigree data of Valle del Belice dairy sheep breed. |
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Palavras-Chave: |
Teste de paternidade. |
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Thesagro: |
Ovelha. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01704naa a2200193 a 4500
001 1959030
005 2013-06-24
008 2013 bl uuuu u00u1 u #d
100 1 $aROSA, A. J. de M.
245 $aParentage verification of Valle del Belice dairy sheep using multiplex microsatellite panel.
260 $c2013
520 $aThe aim of this work was to develop and evaluate a PCR based microsatellite markers multiplex system for parentage verification of Sicilian Valle del Belice dairy sheep. A total of 85 samples of blood and hair were collected and genotyped for 24 microsatellite markers in multiplex electrophoresis runs. A total of 269 alleles were detected across the 24 loci investigated. The PIC considering all loci was equal to 0.736, showing that this microsatellite panel was very polymorphic and highly informative. A parentage test was performed on 64 families generated with multiple sires. Results indicated 20.3% and 29.7% misidentification rates for females and males, respectively. In 8 cases, out of 13 maternal exclusions, the real mother was identified among other females within the flock. The observed misidentification rates indicated the necessity of keeping more efficient collection of genealogical records, in order to properly control inbreeding or implement a breeding program. The parentage test presented here could be a helpful tool on verifying or even reconstructing the current pedigree data of Valle del Belice dairy sheep breed.
650 $aOvelha
653 $aTeste de paternidade
700 1 $aSARDINA, M. T.
700 1 $aMASTRANGELO, S.
700 1 $aTOLONE, M.
700 1 $aPORTOLANO, B.
773 $tSmall Ruminant Research$gv. 113, n. 1, p. 62-65, June 2013.
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