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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
26/07/2017 |
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Data da última atualização: |
18/10/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
LOURENÇO. A. M. S.; SANTOS, C. A. F.; SILVA, A. M. da; COSTA, S. R. da. |
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Afiliação: |
ANDRESSA MIRELLE SANTOS LOURENÇO, Estagiária da Embrapa Semiárido; CARLOS ANTONIO FERNANDES SANTOS, CPATSA; JAMILLE CARDEAL DA SILVA, Bolsista PIBIC-CNPq; SONIANE RODRIGUES DA COSTA, Tecnóloga em Gestão em Fruticultura Irrigada. |
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Título: |
Seleção molecular assistida para a identificação de alelo para cor rósea em população de cebola roxa. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 12., 2017, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2017. |
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Páginas: |
p. 135-139. |
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Série: |
(Embrapa Semiárido. Documentos, 279). |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi aplicar o marcador molecular ANS para genotipar plantas de população de cebola roxa para possibilitar a eliminação de alelos recessivos da cor rósea. |
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Palavras-Chave: |
Cebola roxa; Onion; Seleção assistida. |
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Thesagro: |
Allium Cepa; Cebola; Hortaliça; Marcador Genético; Marcador molecular. |
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Thesaurus Nal: |
Genetic markers. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162116/1/Artigo-17.pdf
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Marc: |
LEADER 01098nam a2200277 a 4500
001 2073216
005 2018-10-18
008 2017 bl uuuu u00u1 u #d
100 1 $aLOURENÇO. A. M. S.
245 $aSeleção molecular assistida para a identificação de alelo para cor rósea em população de cebola roxa.$h[electronic resource]
260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 12., 2017, Petrolina. Anais... Petrolina: Embrapa Semiárido$c2017
300 $ap. 135-139.
490 $a(Embrapa Semiárido. Documentos, 279).
520 $aO objetivo deste trabalho foi aplicar o marcador molecular ANS para genotipar plantas de população de cebola roxa para possibilitar a eliminação de alelos recessivos da cor rósea.
650 $aGenetic markers
650 $aAllium Cepa
650 $aCebola
650 $aHortaliça
650 $aMarcador Genético
650 $aMarcador molecular
653 $aCebola roxa
653 $aOnion
653 $aSeleção assistida
700 1 $aSANTOS, C. A. F.
700 1 $aSILVA, A. M. da
700 1 $aCOSTA, S. R. da
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Registro original: |
Embrapa Semiárido (CPATSA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/12/2015 |
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Data da última atualização: |
05/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
KUMA, K. M.; LOPES-CAITAR, V. S.; ROMERO, C. C. T.; SILVA, S. M. H.; CARVALHO, M. C. C. G.; ABDELNOOR, R. V.; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. |
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Afiliação: |
UEL; UEL; CNPSo; UEL; UENP; RICARDO VILELA ABDELNOOR, CNPSO; WALDIR PEREIRA DIAS, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO. |
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Título: |
A high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 34, n. 11, p. 1987-2000, 2015. |
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ISSN: |
1432-203X |
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DOI: |
10.1007/s00299-015-1845-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Gene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection. MenosGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis i... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02326naa a2200241 a 4500
001 2031887
005 2017-08-05
008 2015 bl uuuu u00u1 u #d
022 $a1432-203X
024 7 $a10.1007/s00299-015-1845-2$2DOI
100 1 $aKUMA, K. M.
245 $aA high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis.$h[electronic resource]
260 $c2015
520 $aGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection.
650 $aSoja
700 1 $aLOPES-CAITAR, V. S.
700 1 $aROMERO, C. C. T.
700 1 $aSILVA, S. M. H.
700 1 $aCARVALHO, M. C. C. G.
700 1 $aABDELNOOR, R. V.
700 1 $aDIAS, W. P.
700 1 $aMARCELINO-GUIMARÃES, F. C.
773 $tPlant Cell Reports$gv. 34, n. 11, p. 1987-2000, 2015.
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Embrapa Soja (CNPSO) |
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