Data/hora: 23/09/2024 03:03:20

Biblioteca(s): 	Embrapa Arroz e Feijão.	
Data corrente: 	26/09/2017	
Data da última atualização: 	18/04/2024	
Autoria: 	DUNLAP, C. A.; MASCARIN, G. M.; ROMAGNOLI, E. M.; JACKSON, M. A.	
Afiliação: 	CHRISTOPHER A. DUNLAP, USDA, Peoria-IL; GABRIEL MOURA MASCARIN, CNPAF; EMILIANA M. ROMAGNOLI, USDA, Peoria-IL; MARK A. JACKSON, USDA, Peoria-IL.	
Título: 	Rapid discrimination of Isaria javanica and Isaria poprawskii from Isaria spp. using high resolution DNA melting assays.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Journal of Invertebrate Pathology, v. 150, p. 88-93, 2017.	
ISSN: 	0022-2011	
DOI: 	10.1016/j.jip.2017.09.011	
Idioma: 	Inglês	
Conteúdo: 	The current study evaluates the potential of using high resolution DNA melting assays to discriminate species in the genus Isaria. The study utilizes a previously identified 103 base pair PCR amplicon, which was reported to be selective for Isaria fumosorosea. Our study finds the amplicon selective for Isaria javanica and Isaria poprawskii when assayed against all members of the genus. In addition, the high resolution melting profile of this amplicon can be used to discriminate between I. javanica, I. poprawskii and a 1:1 mixture of the two species. The practical application of this technique was confirmed using a bioassay on whitefly nymphs (Bemisia tabaci biotype B) inoculated with I. javanica, I. poprawskii or a 1:1 mixture of the two species. This assay provides a simple assay to identify these two species of entomopathogenic fungi.	
Palavras-Chave: 	Isaria javanica; Isaria poprawskii; SCAR marker.	
Thesagro: 	Controle biológico; Taxonomia.	
Thesaurus Nal: 	Biological control; Cordycipitaceae; Taxonomy.	
Categoria do assunto: 	O Insetos e Entomologia	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (34812-1) - UPC - AP - DD - 2017 -  - 2017 - DIS