Data/hora: 20/06/2024 22:20:53

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 15/05/2013
Data da última atualização: | 06/09/2017
Autoria: | MARTÍNEZ OCHOA, E. del C.; ANDRADE-RODRIGUEZ, M.; ROCANDIO RODRIGUEZ, M.; VILLEGAS MONTER, A.
Afiliação: | ELISA DEL CARMEN MARTÍNEZ OCHOA, Colegio de Postgraduados; MARÍA ANDRADE-RODRIGUEZ, Universidad Autónoma del Estado de Morelos/Facultad de Ciencias Agropecuarias; MARIO ROCANDIO RODRIGUEZ, Colegio de Postgraduados; ANGEL VILLEGAS MONTER, Colegio de Postgraduados.
Título: | Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars.
Ano de publicação: | 2012
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 11, p. 1629-1636, nov. 2012.
ISSN: | 0100-204X
Idioma: | Inglês
Notas: | Título em português: Identificação de plântulas zigóticas e nucelares em cultivares de manga poliembriônicas.
Conteúdo: | The objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in seven of nine 'Manila' polyembryonic seeds, and in four of seven 'Ataulfo' ones. Also, in polyembryonic seeds not all zygotic seedlings were produced by small embryos located at the micropyle.
Palavras-Chave: | Funículo.
Thesagro: | Cultura de embrião; Mangifera Indica; Marcador molecular; Polimorfismo.
Thesaurus Nal: | Embryo culture; Genetic markers; Polymorphism.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/82846/1/IDENTIFICATION.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (54545-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS