Data/hora: 24/06/2024 15:59:42

Biblioteca(s): | Embrapa Florestas.
Data corrente: | 24/10/2019
Data da última atualização: | 24/10/2019
Autoria: | RODRIGUES, E. M.; KARP, S. G.; MALUCELLI, L. C.; HELM, C. V.; ALVAREZ, T. M.
Afiliação: | Euderléia M. Rodrigues, Universidade Positivo; Susan G. Karp, UFPR; Lucca C. Malucelli, Universidade Positivo; CRISTIANE VIEIRA HELM, CNPF; Thabata M. Alvarez, Universidade Positivo.
Título: | Evaluation of laccase production by Ganoderma lucidum in submerged and solid-state fermentation using different inducers.
Ano de publicação: | 2019
Fonte/Imprenta: | Journal of Basic Microbiology, v. 59, n. 8, p. 784-791, Aug. 2019.
DOI: | 10.1002/jobm.201900084
Idioma: | Inglês
Conteúdo: | Laccases are multicopper oxidases with high potential for industrial applications. Several basidiomycete fungi are natural producers of this enzyme; however, the optimization of production and selection of inducers for increased productivity coupled with low costs is necessary. Lignocellulosic residues are important lignin sources and potential inducers for laccase production. Pinus taeda, a dominant source of wood?based products, has not been investigated for this purpose yet. The aim of this study was to evaluate the production of laccase by the basidiomycete fungus Ganoderma lucidum in the presence of different inducers in submerged and solid?state fermentation. The results of submerged fermentation in presence of 5 ?M CuSO4, 2mM ferulic acid, 0.1 g/L P. taeda sawdust, or 0.05 g/L Kraft lignin indicated that although all the tested inducers promoted increase in laccase activity in specific periods of time, the presence of 2mM ferulic acid resulted in the highest value of laccase activity (49 U/L). Considering the submerged fermentation, experimental design following the Plackett?Burman method showed that the concentrations of ferulic acid and P. taeda sawdust had a significant influence on the laccase activity. The highest value of 785 U/L of laccase activity on submerged fermentation was obtained on the seventh day of cultivation. Finally, solid?state fermentation cultures in P. taeda using ferulic acid or CuSO4 as inducers resulted in enzymatic activities of 144.62 and 149.89 U/g, respectively, confirming the potential of this approach for laccase production by G. lucidum.
Palavras-Chave: | Lacase.
Thesagro: | Pinus Taeda.
Thesaurus Nal: | Ganoderma lucidum; Laccase; Solid state fermentation; Submerged fermentation.
Categoria do assunto: | --
Registro original: | Embrapa Florestas (CNPF)
Exemplares: | CNPF - (57005-1) - UPC - AP - DD -  -  -  - DIS