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| 18. |  | PIRES, C.; FONTES, E.; SUJII, E. R. Porque as abelhas devem ser consideradas nas avaliações de risco de plantas GM: avaliação de exposição, toxicidade e fluxo gênico. In:CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. ResumoID:1347-4.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | CAPALBO, D. M. F.; DUSI, A. N.; SUJII, E. R. Biossegurança no desenvolvimento e uso das plantas transgênicas. Summa Phytopathologica, Botucatu, v.33, n.1, supl., p.129-132, 2007. Edição dos Resumos do XXX Congresso Paulista de Fitopatologia, Botucatu, 2007.| Biblioteca(s): Embrapa Meio Ambiente; Embrapa Recursos Genéticos e Biotecnologia. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
07/12/2021 |
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Data da última atualização: |
10/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LISEI-DE-SÁ, M. e; RODRIGUES‑SILVA, P. L.; MORGANTE, C. V.; MELO, B. P. de; LOURENCO, I. T.; ARRAES, F. B. M.; SOUSA, J. P. A.; GALBIERI, R.; AMORIM, R. M. S.; LINS, C. B. J. de; MACEDO, L. L. P. de; MOREIRA, V. J.; FERREIRA, G. F.; RIBEIRO, T. P.; FRAGOSO, R. da R.; SILVA, M. C. M. da; ALMEIDA-ENGLER, J. de; SA, M. F. G. de. |
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Afiliação: |
MARIA E LISEI-DE-SÁ, Empresa de Pesquisa Agropecuária de Minas Gerais; PAOLO L. RODRIGUES‑SILVA, UCB; CAROLINA VIANNA MORGANTE, CPATSA; BRUNO PAES DE MELO, INCT PlantStress Biotech; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; FABRICIO B. M. ARRAES, INCT PlantStress Biotech; JOÃO P. A. SOUSA, UCB; RAFAEL GALBIERI, Instituto Matogrossense do Algodão; REGINA M. S. AMORIM; CAMILA B. J. DE LINS; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; VALDEIR J. MOREIRA, UNB; GILANNA F. FERREIRA; THUANNE P. RIBEIRO, INCT PlantStress Biotech; RODRIGO DA ROCHA FRAGOSO, CPAC; MARIA CRISTINA MATTAR DA SILVA, Cenargen; JANICE DE ALMEIDA-ENGLER, UMR Institut Sophia Agrobiotech INRA/CNRS/UNS, France; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Pyramiding dsRNAs increases phytonematode tolerance in cotton plants. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Planta, v. 254, 2021. |
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DOI: |
https://doi.org/10.1007/s00425-021-03776-0 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabela T. Lourenço-Tessutti; Leonardo L. P. Macedo; Rodrigo R. Fragoso; Maria C. M. Silva; Maria F. Grossi-de-Sa. |
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Conteúdo: |
Root-knot nematodes (RKN) represent one of the most damaging plant-parasitic nematode genera worldwide. RNAi-mediated suppression of essential nematode genes provides a novel biotechnological strategy for the development of sustainable pest-control methods. Here, we used a Host Induced Gene Silencing (HIGS) approach by stacking dsRNA sequences into a T-DNA construct to target three essential RKN genes: cysteine protease (Mi-cpl), isocitrate lyase (Mi-icl), and splicing factor (Mi-sf), called dsMinc1, driven by the pUceS8.3 constitutive soybean promoter. Transgenic dsMinc1-T4 plants infected with Meloidogyne incognita showed a signifcant reduction in gall formation (57?64%) and egg masses production (58?67%), as well as in the estimated reproduction factor (60?78%), compared with the susceptible non-transgenic cultivar. Galls of the RNAi lines are smaller than the wild-type (WT) plants, whose root systems exhibited multiple welldeveloped root swellings. Transcript levels of the three RKN-targeted genes decreased 13- to 40-fold in nematodes from transgenic cotton galls, compared with those from control WT galls. Finally, the development of non-feeding males in transgenic plants was 2?6 times higher than in WT plants, indicating a stressful environment for nematode development after RKN gene silencing. Data strongly support that HIGS of essential RKN genes is an efective strategy to improve cotton plant tolerance. This study presents the frst application of dsRNA sequences to target multiple genes to promote M. incognita tolerance in cotton without phenotypic penalty in transgenic plants. MenosRoot-knot nematodes (RKN) represent one of the most damaging plant-parasitic nematode genera worldwide. RNAi-mediated suppression of essential nematode genes provides a novel biotechnological strategy for the development of sustainable pest-control methods. Here, we used a Host Induced Gene Silencing (HIGS) approach by stacking dsRNA sequences into a T-DNA construct to target three essential RKN genes: cysteine protease (Mi-cpl), isocitrate lyase (Mi-icl), and splicing factor (Mi-sf), called dsMinc1, driven by the pUceS8.3 constitutive soybean promoter. Transgenic dsMinc1-T4 plants infected with Meloidogyne incognita showed a signifcant reduction in gall formation (57?64%) and egg masses production (58?67%), as well as in the estimated reproduction factor (60?78%), compared with the susceptible non-transgenic cultivar. Galls of the RNAi lines are smaller than the wild-type (WT) plants, whose root systems exhibited multiple welldeveloped root swellings. Transcript levels of the three RKN-targeted genes decreased 13- to 40-fold in nematodes from transgenic cotton galls, compared with those from control WT galls. Finally, the development of non-feeding males in transgenic plants was 2?6 times higher than in WT plants, indicating a stressful environment for nematode development after RKN gene silencing. Data strongly support that HIGS of essential RKN genes is an efective strategy to improve cotton plant tolerance. This study presents the frst application of dsRNA sequences to t... Mostrar Tudo |
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Palavras-Chave: |
Fitonematóide; Interfering RNA; Nematóides das galhas. |
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Thesagro: |
Algodão; Gossypium Hirsutum; Meloidogyne Incognita; Nematóide. |
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Thesaurus NAL: |
Gene silencing. |
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Categoria do assunto: |
--
G Melhoramento Genético |
|
Marc: |
LEADER 02991naa a2200445 a 4500
001 2137487
005 2021-12-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00425-021-03776-0$2DOI
100 1 $aLISEI-DE-SÁ, M. e
245 $aPyramiding dsRNAs increases phytonematode tolerance in cotton plants.$h[electronic resource]
260 $c2021
500 $aNa publicação: Isabela T. Lourenço-Tessutti; Leonardo L. P. Macedo; Rodrigo R. Fragoso; Maria C. M. Silva; Maria F. Grossi-de-Sa.
520 $aRoot-knot nematodes (RKN) represent one of the most damaging plant-parasitic nematode genera worldwide. RNAi-mediated suppression of essential nematode genes provides a novel biotechnological strategy for the development of sustainable pest-control methods. Here, we used a Host Induced Gene Silencing (HIGS) approach by stacking dsRNA sequences into a T-DNA construct to target three essential RKN genes: cysteine protease (Mi-cpl), isocitrate lyase (Mi-icl), and splicing factor (Mi-sf), called dsMinc1, driven by the pUceS8.3 constitutive soybean promoter. Transgenic dsMinc1-T4 plants infected with Meloidogyne incognita showed a signifcant reduction in gall formation (57?64%) and egg masses production (58?67%), as well as in the estimated reproduction factor (60?78%), compared with the susceptible non-transgenic cultivar. Galls of the RNAi lines are smaller than the wild-type (WT) plants, whose root systems exhibited multiple welldeveloped root swellings. Transcript levels of the three RKN-targeted genes decreased 13- to 40-fold in nematodes from transgenic cotton galls, compared with those from control WT galls. Finally, the development of non-feeding males in transgenic plants was 2?6 times higher than in WT plants, indicating a stressful environment for nematode development after RKN gene silencing. Data strongly support that HIGS of essential RKN genes is an efective strategy to improve cotton plant tolerance. This study presents the frst application of dsRNA sequences to target multiple genes to promote M. incognita tolerance in cotton without phenotypic penalty in transgenic plants.
650 $aGene silencing
650 $aAlgodão
650 $aGossypium Hirsutum
650 $aMeloidogyne Incognita
650 $aNematóide
653 $aFitonematóide
653 $aInterfering RNA
653 $aNematóides das galhas
700 1 $aRODRIGUES‑SILVA, P. L.
700 1 $aMORGANTE, C. V.
700 1 $aMELO, B. P. de
700 1 $aLOURENCO, I. T.
700 1 $aARRAES, F. B. M.
700 1 $aSOUSA, J. P. A.
700 1 $aGALBIERI, R.
700 1 $aAMORIM, R. M. S.
700 1 $aLINS, C. B. J. de
700 1 $aMACEDO, L. L. P. de
700 1 $aMOREIRA, V. J.
700 1 $aFERREIRA, G. F.
700 1 $aRIBEIRO, T. P.
700 1 $aFRAGOSO, R. da R.
700 1 $aSILVA, M. C. M. da
700 1 $aALMEIDA-ENGLER, J. de
700 1 $aSA, M. F. G. de
773 $tPlanta$gv. 254, 2021.
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