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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
15/01/2018 |
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Data da última atualização: |
12/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MUNK, M.; ZANETTE, R. de S. S.; CAMARGO, L. S. de A.; SOUZA, N. L. G. D. de; ALMEIDA, C. G. de; GERN, J. C.; GUIMARAES, A. S.; LADEIRA, L. O.; OLIVEIRA, L. F. C. de; BRANDAO, H. de M. |
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Afiliação: |
Michele Munk, UFJF; Rafaella de Souza Salomão Zanette, UFJF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Nelson Luís Gonçalves Dias de Souza, Universidade Federal de Tocantins; Camila Guimaraes de Almeida; JULIANA CARINE GERN, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; Luiz Orlando Ladeira, UFMG; Luiz Fernando Cappa de Oliveira, UFJF; HUMBERTO DE MELLO BRANDAO, CNPGL. |
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Título: |
Using carbon nanotubes to deliver genes to hard-to-transfect mammalian primary fibroblast cells. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Biomedical Physics & Engineering Express, v. 3, n. 4, 045002, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
The insertion of DNA into primary mammalian cells is an important step towards gene therapy and production of transgenic animals. Recently, carbon nanotubes have been explored as an efficient novel non-viral system for delivering genes to cells due to their unique structure and properties. However, their potential for transfecting primary bovine cells in non-toxic concentrations has not been tested. The purpose of this study was to evaluate the cytotoxicity of carboxylic acid-functionalized multiwalled carbon nanotubes (COOH-MWCNTs) and their use to deliver plasmid DNA encoding the gene of green fluorescent protein to bovine primary fibroblast cells. Flow-cytometry cell viability results have shown the non-toxic nature of COOH-MWCNTs at low concentrations. The frequency shifts in Raman spectroscopy showed that the plasmid DNA connects to the nanomaterial. Fluorescence imaging, flow-cytometry and PCR analysis confirmed that the COOH-MWCNT nanovectors delivered pDNA into primary fibroblast cells successfully. The results show that COOH-MWCNTs can be attractive alternatives for delivery of DNA into hard-to-transfect primary bovine cells. |
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Palavras-Chave: |
COOH-MWCNTs; Delivery of DNA. |
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Thesaurus Nal: |
cytotoxicity; genetic improvement. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 01992naa a2200277 a 4500
001 2085277
005 2023-05-12
008 2017 bl uuuu u00u1 u #d
100 1 $aMUNK, M.
245 $aUsing carbon nanotubes to deliver genes to hard-to-transfect mammalian primary fibroblast cells.$h[electronic resource]
260 $c2017
520 $aThe insertion of DNA into primary mammalian cells is an important step towards gene therapy and production of transgenic animals. Recently, carbon nanotubes have been explored as an efficient novel non-viral system for delivering genes to cells due to their unique structure and properties. However, their potential for transfecting primary bovine cells in non-toxic concentrations has not been tested. The purpose of this study was to evaluate the cytotoxicity of carboxylic acid-functionalized multiwalled carbon nanotubes (COOH-MWCNTs) and their use to deliver plasmid DNA encoding the gene of green fluorescent protein to bovine primary fibroblast cells. Flow-cytometry cell viability results have shown the non-toxic nature of COOH-MWCNTs at low concentrations. The frequency shifts in Raman spectroscopy showed that the plasmid DNA connects to the nanomaterial. Fluorescence imaging, flow-cytometry and PCR analysis confirmed that the COOH-MWCNT nanovectors delivered pDNA into primary fibroblast cells successfully. The results show that COOH-MWCNTs can be attractive alternatives for delivery of DNA into hard-to-transfect primary bovine cells.
650 $acytotoxicity
650 $agenetic improvement
653 $aCOOH-MWCNTs
653 $aDelivery of DNA
700 1 $aZANETTE, R. de S. S.
700 1 $aCAMARGO, L. S. de A.
700 1 $aSOUZA, N. L. G. D. de
700 1 $aALMEIDA, C. G. de
700 1 $aGERN, J. C.
700 1 $aGUIMARAES, A. S.
700 1 $aLADEIRA, L. O.
700 1 $aOLIVEIRA, L. F. C. de
700 1 $aBRANDAO, H. de M.
773 $tBiomedical Physics & Engineering Express$gv. 3, n. 4, 045002, 2017.
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Embrapa Gado de Leite (CNPGL) |
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| 1. |  | AVILA, R. I. de; FENTEM, j.; VILLELA, I.; SOMLO, D.; ALMEIDA, A. M. F.; MENDES-GIANNINI, M. J. S.; CANAVEZ, A. D. P. M.; BOSQUETTI, B.; CATARINO, C. M.; SCHUCK, D. C.; VALADARES, B. N.; FACCHINI, G.; MARIGLIANI, B.; FIGUEIRA, A. C. M.; HICKSON, R.; LEME, D. M.; TAGLIATI, C.; SOUZA, L. C. R. de; ENGLER, S. S. M.; CORDEIRO, L. R. G.; KOEPP, J.; GRANJEIRO, J. M.; BRANDAO, H. de M.; MUNK, M.; MATTOS, K. A. de; PEDRALLI, B.; RODRIGUES, M. M. S. F.; STIVAL, A. C.; ANDRADE, J.; BRITO, L. B.; SANTOS, T. R. M. dos; LEITE, J.; SILVA, A. C. G. da; VALADARES, M. C. Brazilian National Network of Alternative Methods (RENAMA) 10th anniversary: meeting of the associated laboratories, may 2022. Alternatives to Laboratory Animals, v. 52, n. 1, p. 60-68, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
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