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| Registros recuperados : 31 | |
| 6. |  | FREITAS, L. E. L. de; SILVA, T. S. de C.; FRACALOSSI, D. M. Protein to energy ratios and cost, performance, and ammonia excretion in juvenile jundiá (Rhamdia quelen), South American catfish. Scientia Agricola, v.78, Suppl., e20200288, 2021.| Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Pesca e Aquicultura. |
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| 7. | | FREITAS, L. E. L. de; INOUE, D. Y.; OLIVEIRA, L. L. de; NUNES, M. E.; FRACALOSSI, D. M. Coeficientes de digestibilidade aparente de diferentes fontes proteicas para juvenis de jundiá (Rhamdia quelen). In: FENACAM & LACQUA/SARA (WAS)'15.; LATIN AMERICAN & CARIBBEAN AQUACULTURE 15.; SOUTH AMERICAN REGIONAL AQUACULTURE 15.; INTERNATIONAL SHRIMP FARMING SYMPOSIUM, 12.; INTERNATIONAL ACQUACULTURE TRADE SHOW, 12.; INTERNATIONAL AQUACULTURE SYMPOSIUM, 9.; TILAPIA ECONOMIC FORUM, 3., 2015, Fortaleza. Abstracts... Fortaleza: ABCC: World Aquaculture Society, 2015. p. 287.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 10. | | NUNES, M. E.; OLIVEIRA, L. L. de; INOUE, D. Y.; FREITAS, L. E. L. de; FRACALOSSI, D. M. Aminoácidos limitantes em diferentes fontes proteicas na alimentação de juvenis de jundiá (Rhamdia quelen). In: FENACAM & LACQUA/SARA (WAS)'15.; LATIN AMERICAN & CARIBBEAN AQUACULTURE 15.; SOUTH AMERICAN REGIONAL AQUACULTURE 15.; INTERNATIONAL SHRIMP FARMING SYMPOSIUM, 12.; INTERNATIONAL ACQUACULTURE TRADE SHOW, 12.; INTERNATIONAL AQUACULTURE SYMPOSIUM, 9.; TILAPIA ECONOMIC FORUM, 3., 2015, Fortaleza. Abstracts... Fortaleza: ABCC: World Aquaculture Society, 2015. p. 387.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 11. | | OLIVEIRA, L. L.; INOUE, D. Y.; PIRES, J. J.; FREITAS, L. E. L. de; MACHADO, C.; FRACALOSSI, D. M. Excreção de nitrogênio amoniacal total em juvenis de jundiá Rhamdia quelen alimentados com dietas contendo diferentes relações de energia: proteina. In: CONGRESSO BRASILEIRO DE ENGENHARIA DE PESCA, 20., 2017, Florianópolis. Tecnologia e inovação para pesca e aquicultura: anais. Florianópolis: FAEP-BR, 2017. p. 145.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 13. | | VILLELA, L. C. V.; SCHUCH, A. C.; ALVES, A. L.; VARELA, E. S.; KIRSCHNIK, L. N. G.; BELCHIOR, L. S.; FREITAS, L. E. L. de; MATAVELI, M. Implantação do programa de conservação de recursos genéticos de peixes nativos comerciais da Amazônia na Embrapa. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 4., 2016, Curitiba. Recursos genéticos no Brasil: a base para o desenvolvimento sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2016. p. 85.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 14. | | PIEDADE, A. E.; FREITAS, L. E. L. de; VIDAL, L. V. O.; ANDRADE, G. de S.; FRECHIANI, G. D. J.; TORATI, L. S. Efeito do farelo de Kappaphycus alvarezii no desempenho zootécnico e nas respostas fisiológicas de juvenis de tambaqui. In: CONGRESSO BRASILEIRO DE AQUICULTURA E BIOLOGIA AQUÁTICA, 10., 2023, Florianópolis. Conservar, produzir e inovar: anais eletrônicos. Florianópolis: Aquabio, 2023. Aquaciência 2023.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 16. | | SHIOTSUKI, L.; ATAIDES, K. da S.; KIRSCHNIK, L. N. G.; REZENDE, F. P.; FREITAS, L. E. L. de; VARELA, E. S.; VILLELA, L. C. V.; TORATI, L. S. Estimativas de parâmetros genéticos para medidas morfométrica em juvenis de tambaqui. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 13., 2019, Salvador, BA. Anais. Salvador: SBMA, 2019.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 20. | | VILLELA, L. C. V.; KIRSCHNIK, L. N. G.; VARELA, E. S.; ROUTLEDGE, E. A. B.; REZENDE, F. P.; TORATI, L. S.; SHIOTSUKI, L.; FREITAS, L. E. L. Programa de conservação de recursos genéticos de peixes nativos de interesse econômico da Embrapa. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 6., 2020. Recursos genéticos e bioeconomia: inovação para um futuro sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2020.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| Registros recuperados : 31 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
31/03/2009 |
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Data da última atualização: |
20/12/2023 |
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Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
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Autoria: |
RODRIGUES, J. C. M. |
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Afiliação: |
Julio Carlyle Macedo Rodrigues, Embrapa Recursos Genéticos e Biotecnologia. |
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Título: |
Epigenetic regulation and inheritance of autonomous seed development in apomictic. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
2007. |
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Páginas: |
217 p. |
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Idioma: |
Inglês |
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Notas: |
Tese (Doutorado em Filosofia) - University of Adelaide. Orientadora: Anna M. G. Koltunow. Co-orientadora: Vera Tavares de Campos Carneiro, Embrapa Recursos Genéticos e Biotecnologia. |
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Conteúdo: |
Apomixis is an intriguing and agronomically valuable asexual reproductive pathway resulting in seeds that give rise to plants that are identical in genotype to the female parent. Apomixis is absent in agriculturally important seed crops and our work has focused on the analysis of apomixis in the daisy-like genus Hieracium which contains sexual and apomictic species. Prior studies have shown that apomixis in Hieracium is controlled by two dominant loci. The LOA controls the avoidance of meiosis during female gametophyte (embryo sac) formation and the LOP locus is required for fertilization independent embryo formation during seed initiation. The genes conferring apomixis are unknown. In this study we focused on the events of autonomous seed initiation. Cytological examination of apomictic mutants that have lost LOA or LOP and analysis of their progeny enabled us to characterize developmental aspects associated with the function of these loci. Upon removal of LOA meiosis occurs normally and LOP segregates with a 1:1 ratio in the progeny, characterizing maternal gametophytic control. We also show that autonomous embryo formation segregates with autonomous endosperm formation, suggesting that these two loci are closely linked. However, upon meiotic division, embryo lethal components arise and embryo development in apomeiosis mutants was generally defective and seed set was low. Similarly, upon removal of LOP, apomixis initiation occurs normally and unreduced embryo sacs can only form seeds if pollinated. Autonomous seed initiation is actively repressed in the sexual model plant Arabidopsis by the action of a chromatin remodelling complex encoded by the FERTILIZATION INDEPENDENT SEED (FIS)-class genes. These genes are homologues of the Drosophila PcG complex that also repress gene expression throughout Drosophila development. Mutations in the FIS-class genes lead to elements of apomixis, sueh as autonomous endosperm, and in one particular mutant, autonomous egg cell development. Given the similarity in apomictic and FIS-class gene mutant phenotype we isolated three homologues from sexual and apomictic Hieracium plants: FERTILIZATION INDEPENDENT ENDOSPERM (FIE), MULTICOPY SUPPRESSOR OF IRA1 (MS11) AND RETINOBLASTOMA (RBR). FIS-class genes from sexual and apomicit Hieracium and examined their expression, interaction and function during seed initiation. The isolated Hieracium FIS-class genes were highly conserved in sexual and apomictic plants in terns of gene sequence and temporal and spatial expression pattern. Analysis of protein interactions by yeast-two hybrid showed that the HFIE gene from sexual and apomictic plants does not interact with other complex members in the same manner found in Arabidopsis. Protein modelling uncovered structural differences between the Arabidopsis and Hieracium FIE proteins. RNAi- mediated down-regulation of HFIE in sexual Hieracium did not lead to autonomous seed initiation indicating HFIE was not part of a repressive complexo Down-regulation of HFIE in sexual and apomictic plants revealed the gene was essential for embryo growth and viability. Therefore, FIS-complex genes interact differently in Arabidopsis and Hieracium and have different developmental roles. In summary, the results presented here suggest that the FIS-genes are not mutated in apomictic Hieracium plants, but they interact differently relative to the Arabidopsis counterparts and play a fundamental role in embryogenesis. Thus, engineering autonomous seed into crops will not depend on mutating these genes but rather in uncovering the molecular signal that triggers apomictic development. MenosApomixis is an intriguing and agronomically valuable asexual reproductive pathway resulting in seeds that give rise to plants that are identical in genotype to the female parent. Apomixis is absent in agriculturally important seed crops and our work has focused on the analysis of apomixis in the daisy-like genus Hieracium which contains sexual and apomictic species. Prior studies have shown that apomixis in Hieracium is controlled by two dominant loci. The LOA controls the avoidance of meiosis during female gametophyte (embryo sac) formation and the LOP locus is required for fertilization independent embryo formation during seed initiation. The genes conferring apomixis are unknown. In this study we focused on the events of autonomous seed initiation. Cytological examination of apomictic mutants that have lost LOA or LOP and analysis of their progeny enabled us to characterize developmental aspects associated with the function of these loci. Upon removal of LOA meiosis occurs normally and LOP segregates with a 1:1 ratio in the progeny, characterizing maternal gametophytic control. We also show that autonomous embryo formation segregates with autonomous endosperm formation, suggesting that these two loci are closely linked. However, upon meiotic division, embryo lethal components arise and embryo development in apomeiosis mutants was generally defective and seed set was low. Similarly, upon removal of LOP, apomixis initiation occurs normally and unreduced embryo sacs can only... Mostrar Tudo |
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Thesagro: |
Apomixia; Reprodução. |
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Thesaurus NAL: |
Hieracium. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 04273nam a2200169 a 4500
001 1167799
005 2023-12-20
008 2007 bl uuuu m 00u1 u #d
100 1 $aRODRIGUES, J. C. M.
245 $aEpigenetic regulation and inheritance of autonomous seed development in apomictic.$h[electronic resource]
260 $a2007.$c2007
300 $a217 p.
500 $aTese (Doutorado em Filosofia) - University of Adelaide. Orientadora: Anna M. G. Koltunow. Co-orientadora: Vera Tavares de Campos Carneiro, Embrapa Recursos Genéticos e Biotecnologia.
520 $aApomixis is an intriguing and agronomically valuable asexual reproductive pathway resulting in seeds that give rise to plants that are identical in genotype to the female parent. Apomixis is absent in agriculturally important seed crops and our work has focused on the analysis of apomixis in the daisy-like genus Hieracium which contains sexual and apomictic species. Prior studies have shown that apomixis in Hieracium is controlled by two dominant loci. The LOA controls the avoidance of meiosis during female gametophyte (embryo sac) formation and the LOP locus is required for fertilization independent embryo formation during seed initiation. The genes conferring apomixis are unknown. In this study we focused on the events of autonomous seed initiation. Cytological examination of apomictic mutants that have lost LOA or LOP and analysis of their progeny enabled us to characterize developmental aspects associated with the function of these loci. Upon removal of LOA meiosis occurs normally and LOP segregates with a 1:1 ratio in the progeny, characterizing maternal gametophytic control. We also show that autonomous embryo formation segregates with autonomous endosperm formation, suggesting that these two loci are closely linked. However, upon meiotic division, embryo lethal components arise and embryo development in apomeiosis mutants was generally defective and seed set was low. Similarly, upon removal of LOP, apomixis initiation occurs normally and unreduced embryo sacs can only form seeds if pollinated. Autonomous seed initiation is actively repressed in the sexual model plant Arabidopsis by the action of a chromatin remodelling complex encoded by the FERTILIZATION INDEPENDENT SEED (FIS)-class genes. These genes are homologues of the Drosophila PcG complex that also repress gene expression throughout Drosophila development. Mutations in the FIS-class genes lead to elements of apomixis, sueh as autonomous endosperm, and in one particular mutant, autonomous egg cell development. Given the similarity in apomictic and FIS-class gene mutant phenotype we isolated three homologues from sexual and apomictic Hieracium plants: FERTILIZATION INDEPENDENT ENDOSPERM (FIE), MULTICOPY SUPPRESSOR OF IRA1 (MS11) AND RETINOBLASTOMA (RBR). FIS-class genes from sexual and apomicit Hieracium and examined their expression, interaction and function during seed initiation. The isolated Hieracium FIS-class genes were highly conserved in sexual and apomictic plants in terns of gene sequence and temporal and spatial expression pattern. Analysis of protein interactions by yeast-two hybrid showed that the HFIE gene from sexual and apomictic plants does not interact with other complex members in the same manner found in Arabidopsis. Protein modelling uncovered structural differences between the Arabidopsis and Hieracium FIE proteins. RNAi- mediated down-regulation of HFIE in sexual Hieracium did not lead to autonomous seed initiation indicating HFIE was not part of a repressive complexo Down-regulation of HFIE in sexual and apomictic plants revealed the gene was essential for embryo growth and viability. Therefore, FIS-complex genes interact differently in Arabidopsis and Hieracium and have different developmental roles. In summary, the results presented here suggest that the FIS-genes are not mutated in apomictic Hieracium plants, but they interact differently relative to the Arabidopsis counterparts and play a fundamental role in embryogenesis. Thus, engineering autonomous seed into crops will not depend on mutating these genes but rather in uncovering the molecular signal that triggers apomictic development.
650 $aHieracium
650 $aApomixia
650 $aReprodução
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