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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
17/06/2008 |
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Data da última atualização: |
24/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BOMFETI, C. A.; SOUZA-PACCOLA, E. A.; MASSOLA JUNIOR, N. S.; MARRIEL, I. E.; MEIRELLES, W. F.; CASELA, C. R.; PACCOLA-MEIRELLES, L. D. |
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Afiliação: |
Cleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL. |
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Título: |
Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008. |
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DOI: |
10.1590/S1982-56762008000100010 |
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Idioma: |
Inglês |
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Conteúdo: |
The etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis. MenosThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. T... Mostrar Tudo |
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Palavras-Chave: |
Doença bacteriana do milho; doenças foliares do milho; Maize bacterial disease; Maize leaf disease. |
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Thesagro: |
Milho; Zea Mays. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32277/1/Localization-pantoea.pdf
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Marc: |
LEADER 02604naa a2200277 a 4500
001 1491172
005 2018-05-24
008 2008 bl uuuu u00u1 u #d
024 7 $a10.1590/S1982-56762008000100010$2DOI
100 1 $aBOMFETI, C. A.
245 $aLocalization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.$h[electronic resource]
260 $c2008
520 $aThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis.
650 $aMilho
650 $aZea Mays
653 $aDoença bacteriana do milho
653 $adoenças foliares do milho
653 $aMaize bacterial disease
653 $aMaize leaf disease
700 1 $aSOUZA-PACCOLA, E. A.
700 1 $aMASSOLA JUNIOR, N. S.
700 1 $aMARRIEL, I. E.
700 1 $aMEIRELLES, W. F.
700 1 $aCASELA, C. R.
700 1 $aPACCOLA-MEIRELLES, L. D.
773 $tTropical Plant Pathology, Brasília$gv. 33, n. 1, p. 63-66, Jan./Feb. 2008.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/12/2014 |
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Data da última atualização: |
26/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ARAUJO, A. G. de; VAL, A. D. B.; SOARES, J. D. R.; RODRIGUES, F. A.; PASQUAL, M.; ROCHA, H. S.; ASMAR, S. A.; CORDEIRO, Z. J. M.; SILVA, S. de O. |
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Afiliação: |
APARECIDA GOMES DE ARAUJO, UFLA; AURINETE DAIENN BORGES DO VAL, UESPI; JOYCE DÓRIA RODRIGUES SOARES, UFLA; FILIPE ALMENDAGNA RODRIGUES, UFLA; MOACIR PASQUAL; HERMINIO SOUZA ROCHA, CNPMF; SIMONE ABREU ASMAR, UFU; ZILTON JOSE MACIEL CORDEIRO, CNPMF; SEBASTIÃO DE OLIVEIRA E SILVA. |
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Título: |
Host-pathogen interactions of Musa spp. and Mycosphaerella musicola with epidemiological variables and leaf anatomy within the pathosystem of Yellow Sigatoka disease. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v. 8, issue 8, Aug 2014. |
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ISSN: |
1835-2707 |
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Idioma: |
Inglês |
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Conteúdo: |
Yellow Sigatoka, which is caused by Mycosphaerella musicola/Pseudocercospora musae, is the primary biotic problem for the Brazilian banana crop, with the causal agent presenting high pathogenic variability among the pathogen isolates. Few details concerning the host-pathogen interactions that occur during the infection process are available for this disease. Studies have been carried out under greenhouse conditions with artificial inoculation with 2 M. musicola isolates (1 - Lavras, Minas Gerais state and 2 - Cruz das Almas, Bahia state) of a group of banana varieties, including 'Grand Naine', 'Prata Ana', 'Calipso', 'Preciosa', 'Japira' and 'BRS Platina'. In each plant, two leaves were inoculated; F1 - Leaf number one and F2 - Leaf number two. After the dynamics of host-pathogen interactions, the different bananas genotypes were evaluated and the fungus-associated changes in the leaf anatomy within the tissues and stomata were determined. Inoculations were carried out with a suspension of 4.104 conidia/mL that was sprayed onto micropropagated plants of the different genotypes. The lower limb surface of the number one leaves (F1) and number two leaves (F2) was sprayed until runoff. The data were collected weekly over ten weeks, although shorter incubation and latent periods were observed in the susceptible varieties (Grand Naine and Prata Ana). The Grand Naine genotype showed the greatest disease severity, confirming its susceptibility to yellow Sigatoka. The leaf inoculation of M. musicola altered the anatomy. In most cases, contamination increases the thickness of the epidermis, hypodermis and parenchyma. However, the thickness of leaf tissues was reduced in Prata Ana. The BRS Platina hybrid showed the greatest variation in these parameters. In general, infection with Mycosphaerella significantly decreased the stomatal density. Exceptions to this behavior occurred in the epidermis of the leaves of Grand Naine and BRS Platina. The resistant genotypes, Preciosa, Japira and BRS Platina, are promising for commercial plantations in regions where the yellow Sigatoka inoculum pressure is high. The isolate from Cruz das Almas, Bahia State, was more pathogenic (AUDSPC=7.9100) than was the one from Lavras, Minas Gerais State (AUDSPC=3.2737), in which the Grand Naine susceptible genotype was inoculated. Yellow Sigatoka infection changed the leaf anatomy by altering the leaf thickness and the number and size of the stomata. MenosYellow Sigatoka, which is caused by Mycosphaerella musicola/Pseudocercospora musae, is the primary biotic problem for the Brazilian banana crop, with the causal agent presenting high pathogenic variability among the pathogen isolates. Few details concerning the host-pathogen interactions that occur during the infection process are available for this disease. Studies have been carried out under greenhouse conditions with artificial inoculation with 2 M. musicola isolates (1 - Lavras, Minas Gerais state and 2 - Cruz das Almas, Bahia state) of a group of banana varieties, including 'Grand Naine', 'Prata Ana', 'Calipso', 'Preciosa', 'Japira' and 'BRS Platina'. In each plant, two leaves were inoculated; F1 - Leaf number one and F2 - Leaf number two. After the dynamics of host-pathogen interactions, the different bananas genotypes were evaluated and the fungus-associated changes in the leaf anatomy within the tissues and stomata were determined. Inoculations were carried out with a suspension of 4.104 conidia/mL that was sprayed onto micropropagated plants of the different genotypes. The lower limb surface of the number one leaves (F1) and number two leaves (F2) was sprayed until runoff. The data were collected weekly over ten weeks, although shorter incubation and latent periods were observed in the susceptible varieties (Grand Naine and Prata Ana). The Grand Naine genotype showed the greatest disease severity, confirming its susceptibility to yellow Sigatoka. The leaf inoculatio... Mostrar Tudo |
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Palavras-Chave: |
Plant diseases; Progress curve disease. |
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Thesagro: |
Anatomia vegetal; Banana. |
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Thesaurus NAL: |
Plant anatomy; yellow Sigatoka. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03396naa a2200301 a 4500
001 2002824
005 2023-05-26
008 2014 bl uuuu u00u1 u #d
022 $a1835-2707
100 1 $aARAUJO, A. G. de
245 $aHost-pathogen interactions of Musa spp. and Mycosphaerella musicola with epidemiological variables and leaf anatomy within the pathosystem of Yellow Sigatoka disease.$h[electronic resource]
260 $c2014
520 $aYellow Sigatoka, which is caused by Mycosphaerella musicola/Pseudocercospora musae, is the primary biotic problem for the Brazilian banana crop, with the causal agent presenting high pathogenic variability among the pathogen isolates. Few details concerning the host-pathogen interactions that occur during the infection process are available for this disease. Studies have been carried out under greenhouse conditions with artificial inoculation with 2 M. musicola isolates (1 - Lavras, Minas Gerais state and 2 - Cruz das Almas, Bahia state) of a group of banana varieties, including 'Grand Naine', 'Prata Ana', 'Calipso', 'Preciosa', 'Japira' and 'BRS Platina'. In each plant, two leaves were inoculated; F1 - Leaf number one and F2 - Leaf number two. After the dynamics of host-pathogen interactions, the different bananas genotypes were evaluated and the fungus-associated changes in the leaf anatomy within the tissues and stomata were determined. Inoculations were carried out with a suspension of 4.104 conidia/mL that was sprayed onto micropropagated plants of the different genotypes. The lower limb surface of the number one leaves (F1) and number two leaves (F2) was sprayed until runoff. The data were collected weekly over ten weeks, although shorter incubation and latent periods were observed in the susceptible varieties (Grand Naine and Prata Ana). The Grand Naine genotype showed the greatest disease severity, confirming its susceptibility to yellow Sigatoka. The leaf inoculation of M. musicola altered the anatomy. In most cases, contamination increases the thickness of the epidermis, hypodermis and parenchyma. However, the thickness of leaf tissues was reduced in Prata Ana. The BRS Platina hybrid showed the greatest variation in these parameters. In general, infection with Mycosphaerella significantly decreased the stomatal density. Exceptions to this behavior occurred in the epidermis of the leaves of Grand Naine and BRS Platina. The resistant genotypes, Preciosa, Japira and BRS Platina, are promising for commercial plantations in regions where the yellow Sigatoka inoculum pressure is high. The isolate from Cruz das Almas, Bahia State, was more pathogenic (AUDSPC=7.9100) than was the one from Lavras, Minas Gerais State (AUDSPC=3.2737), in which the Grand Naine susceptible genotype was inoculated. Yellow Sigatoka infection changed the leaf anatomy by altering the leaf thickness and the number and size of the stomata.
650 $aPlant anatomy
650 $ayellow Sigatoka
650 $aAnatomia vegetal
650 $aBanana
653 $aPlant diseases
653 $aProgress curve disease
700 1 $aVAL, A. D. B.
700 1 $aSOARES, J. D. R.
700 1 $aRODRIGUES, F. A.
700 1 $aPASQUAL, M.
700 1 $aROCHA, H. S.
700 1 $aASMAR, S. A.
700 1 $aCORDEIRO, Z. J. M.
700 1 $aSILVA, S. de O.
773 $tAustralian Journal of Crop Science$gv. 8, issue 8, Aug 2014.
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