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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
24/10/2019 |
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Data da última atualização: |
24/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
RODRIGUES, E. M.; KARP, S. G.; MALUCELLI, L. C.; HELM, C. V.; ALVAREZ, T. M. |
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Afiliação: |
Euderléia M. Rodrigues, Universidade Positivo; Susan G. Karp, UFPR; Lucca C. Malucelli, Universidade Positivo; CRISTIANE VIEIRA HELM, CNPF; Thabata M. Alvarez, Universidade Positivo. |
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Título: |
Evaluation of laccase production by Ganoderma lucidum in submerged and solid-state fermentation using different inducers. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Basic Microbiology, v. 59, n. 8, p. 784-791, Aug. 2019. |
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DOI: |
10.1002/jobm.201900084 |
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Idioma: |
Inglês |
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Conteúdo: |
Laccases are multicopper oxidases with high potential for industrial applications. Several basidiomycete fungi are natural producers of this enzyme; however, the optimization of production and selection of inducers for increased productivity coupled with low costs is necessary. Lignocellulosic residues are important lignin sources and potential inducers for laccase production. Pinus taeda, a dominant source of wood?based products, has not been investigated for this purpose yet. The aim of this study was to evaluate the production of laccase by the basidiomycete fungus Ganoderma lucidum in the presence of different inducers in submerged and solid?state fermentation. The results of submerged fermentation in presence of 5 ?M CuSO4, 2mM ferulic acid, 0.1 g/L P. taeda sawdust, or 0.05 g/L Kraft lignin indicated that although all the tested inducers promoted increase in laccase activity in specific periods of time, the presence of 2mM ferulic acid resulted in the highest value of laccase activity (49 U/L). Considering the submerged fermentation, experimental design following the Plackett?Burman method showed that the concentrations of ferulic acid and P. taeda sawdust had a significant influence on the laccase activity. The highest value of 785 U/L of laccase activity on submerged fermentation was obtained on the seventh day of cultivation. Finally, solid?state fermentation cultures in P. taeda using ferulic acid or CuSO4 as inducers resulted in enzymatic activities of 144.62 and 149.89 U/g, respectively, confirming the potential of this approach for laccase production by G. lucidum. MenosLaccases are multicopper oxidases with high potential for industrial applications. Several basidiomycete fungi are natural producers of this enzyme; however, the optimization of production and selection of inducers for increased productivity coupled with low costs is necessary. Lignocellulosic residues are important lignin sources and potential inducers for laccase production. Pinus taeda, a dominant source of wood?based products, has not been investigated for this purpose yet. The aim of this study was to evaluate the production of laccase by the basidiomycete fungus Ganoderma lucidum in the presence of different inducers in submerged and solid?state fermentation. The results of submerged fermentation in presence of 5 ?M CuSO4, 2mM ferulic acid, 0.1 g/L P. taeda sawdust, or 0.05 g/L Kraft lignin indicated that although all the tested inducers promoted increase in laccase activity in specific periods of time, the presence of 2mM ferulic acid resulted in the highest value of laccase activity (49 U/L). Considering the submerged fermentation, experimental design following the Plackett?Burman method showed that the concentrations of ferulic acid and P. taeda sawdust had a significant influence on the laccase activity. The highest value of 785 U/L of laccase activity on submerged fermentation was obtained on the seventh day of cultivation. Finally, solid?state fermentation cultures in P. taeda using ferulic acid or CuSO4 as inducers resulted in enzymatic activities of 144.62 and ... Mostrar Tudo |
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Palavras-Chave: |
Lacase. |
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Thesagro: |
Pinus Taeda. |
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Thesaurus NAL: |
Ganoderma lucidum; Laccase; Solid state fermentation; Submerged fermentation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02394naa a2200253 a 4500
001 2113463
005 2019-10-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1002/jobm.201900084$2DOI
100 1 $aRODRIGUES, E. M.
245 $aEvaluation of laccase production by Ganoderma lucidum in submerged and solid-state fermentation using different inducers.$h[electronic resource]
260 $c2019
520 $aLaccases are multicopper oxidases with high potential for industrial applications. Several basidiomycete fungi are natural producers of this enzyme; however, the optimization of production and selection of inducers for increased productivity coupled with low costs is necessary. Lignocellulosic residues are important lignin sources and potential inducers for laccase production. Pinus taeda, a dominant source of wood?based products, has not been investigated for this purpose yet. The aim of this study was to evaluate the production of laccase by the basidiomycete fungus Ganoderma lucidum in the presence of different inducers in submerged and solid?state fermentation. The results of submerged fermentation in presence of 5 ?M CuSO4, 2mM ferulic acid, 0.1 g/L P. taeda sawdust, or 0.05 g/L Kraft lignin indicated that although all the tested inducers promoted increase in laccase activity in specific periods of time, the presence of 2mM ferulic acid resulted in the highest value of laccase activity (49 U/L). Considering the submerged fermentation, experimental design following the Plackett?Burman method showed that the concentrations of ferulic acid and P. taeda sawdust had a significant influence on the laccase activity. The highest value of 785 U/L of laccase activity on submerged fermentation was obtained on the seventh day of cultivation. Finally, solid?state fermentation cultures in P. taeda using ferulic acid or CuSO4 as inducers resulted in enzymatic activities of 144.62 and 149.89 U/g, respectively, confirming the potential of this approach for laccase production by G. lucidum.
650 $aGanoderma lucidum
650 $aLaccase
650 $aSolid state fermentation
650 $aSubmerged fermentation
650 $aPinus Taeda
653 $aLacase
700 1 $aKARP, S. G.
700 1 $aMALUCELLI, L. C.
700 1 $aHELM, C. V.
700 1 $aALVAREZ, T. M.
773 $tJournal of Basic Microbiology$gv. 59, n. 8, p. 784-791, Aug. 2019.
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