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| Registros recuperados : 183 | |
| 103. |  | BARBOSA, A. E. A. D.; FR, E. V. S. A.; SILVA, M. C. M. da; SOUZA, D. S. L.; OLIVEIRA NETO, O. B.; VALENCIA, A.; ROCHA, T. L.; SA, M. F. G. de. α-Amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabica plants inhibits α-amylases from the coffee berry borer pest. BMC Biotechnology, v. 10, n. 44, 2010. Disponível em:.Acesso em: 6 jan. 2011.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 104. | | NAKASU, E. Y. T.; FIRMINO, A. A. P.; DIAS, S. C.; ROCHA, T. L.; RAMOS, H. B.; OLIVEIRA, G. R.; LUCENA, W.; CARLINI, C. R.; SA, M. F. G. de. Analysis of Cry8Ka5-binding proteins from Anthonomus grandis (Coleoptera:Curculionidae) midgut. Journal of Invertebrate Pathology, v. 104, p. 227-230, 2010.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 105. | | ROCHA, T. L.; BOUGHTON, B. A.; SIQUEIRA, T.; FIRMINO, A. A. P.; OLDACH, K.; MURRAY, A. G. W.; CALLAHAM, D. L.; SHEEDY, J.; SILVEIRA, R. E.; CARNEIRO, R. M. D. G.; SA, M. F. G. de; BACIC, A.; ROESSNER, U. A comprehensive approach to identify toxic metabolites present in seeds of leguminous antagonistic plants to juveniles of plant parasitic nematode. In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 29., 2011, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Nematologia, 2011. p. 257-258.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 106. | | PINTO, R. W. P.; FERREIRA, J. M.; ROCHA, T. L.; GIANERINI, Y. X.; BALIEIRO, F. de C.; DONAGEMMA, G. K.; COUTINHO, H. L. da C.; ANDRADE, A. G. de. Carbono e nitrogênio em agregados influenciados por diferentes usos em um Cambissolo de Bom Jardim-RJ. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32., 2009, Fortaleza. O solo e a produção de bioenergia: perspectivas e desafios. [Viçosa, MG]: SBCS; Fortaleza: UFC, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Solos. |
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| 107. | | FRANCO, O. L.; ANDRADE, A. E.; ALBUQUERQUE, E. V. S.; GROSSI DE SÁ, M. F.; CARNEIRO, R. M. D. G.; CARNEIRO, R.; EIRA, M. T. S.; ROCHA, T. L.; MEHTA, A. Comparison of different staining methods for coffee proteomic analysis. INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2006.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 108. | | FRANCO, O. L.; ANDRADE, A. E.; BARROS, E. V. S. A.; GROSSI DE SÁ, M. F.; CARNEIRO, R. M. D. G.; CARNEIRO, R.; EIRA, M. T. S.; ROCHA, T. L.; MEHTA, Â. Comparison of different staining methods for coffee proteomic analysis. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2006.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 109. | | FRANCO, O. L.; ANDRADE, A. E.; BARROS, E. V. S. A.; SA, M. F. G. de; CARNEIRO, R. M. D. G.; CARNEIRO, R.; EIRA, M. T. S. da; ROCHA, T. L.; REIS, A. M. dos. Comparison of different staining methods for coffee proteomic analysis. In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.| Biblioteca(s): Embrapa Café. |
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| 110. | | FERREIRA, A. A.; SOLL, C. B.; SILVA, C. P. da; STECKLAND, A. O.; ROCHA, M. R.; BASSO, A. M. M.; CARNEIRO, R. M. D. G.; URBEN, A. F.; ROCHA, T. L.; POLEZ, V. L. P. Cogumelos: uma fonte de compostos bioativos para o controle do fitopatógeno Meloidogyne incógnita. In: SIMPÓSIO INTERNACIONAL SOBRE COGUMELOS NO BRASIL, 7.; SIMPÓSIO NACIONAL SOBRE COGUMELOS COMESTÍVEIS, 6., 2013, Manaus. Anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2013. p. 373-| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 111. | | MARCELINO, L. H.; SOARES, F. X. S.; SILVA, C. M. da; ROCHA, T. L.; CARNEIRO, M.; GANDER, E. S.; SAMPAIO, M. A. M.; CASTRO, L. A. B. de; NESHICH, G. Introduction of tryptophan in the Brazil nut 2s storage albumins by site-directed mutagenesis. In: INTERNATIONAL SYMPOSIUM ON CRYSTALLOGRAPHY AND MOLECULAR BIOLOGY, 1990, Guaruja, SP, Abstracts.[S.l.]: Institute of Advanced Studies, Sao Carlos: Universidade Federal de Sao Carlos, 1990. PS1-03.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 112. | | FRAGOSO, R. da R.; VIANA, A. A. B.; LOURENÇO, I. T.; SOUZA, D. dos S. de L.; LIMA, L. M. de; MEHTA, A.; PINTO, E. R. de C.; ROCHA, T. L.; SÁ, M. F G. de. Interação molecular planta-nematóide. Planaltina, DF: Embrapa Cerrados, 2007. 56 p. (Embrapa Cerrados. Documentos, 198).| Biblioteca(s): Embrapa Gado de Corte. |
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| 113. | | GROSSI DE SÁ, M. F.; DEL SARTO, R. P.; CRUZ, C. C.; RIGDEN, D. J.; OLIVEIRA NETO, O. B.; COUTINHO, M. V.; ROCHA, T. L.; SILVA, M. C. M. Inibidores de α-amilase: novas estratégias para identificação de inibidores com potencial para o controle de inseto-praga. In: CONGRESSO NACIONAL DE BOTÂNICA, 59.; CONGRESSO LATINOAMERICANO Y DEL CACTACESA Y OTRAS SUCULENTAS, 4.; CONGRESS OF INTERNATIONAL SOCIETY FOR SUCULENT PLANT STUDY, 30.; REUNIÃO NORDESTINA DE BOTÂNICA, 31., 2008, São Paulo. Atualidades, desafios e perspectivas da botânica no Brasil. [Porto Alegre]: Sociedade Botânica do Brasil, 2008.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 114. | | SILVA, M. S.; ESPÍNDOLA, L. S.; PAULA, J. E.; ROCHA, T. L.; SILVA, A. C. P.; OLIVEIRA, L. D.; SILVA, L. N.; COSTA, J. V. M.; MURAD, A. M.; CHARCHAR, M. J. d'A.; ANJOS, J. R. N. Fungi, pathogenic to soybean, cotton, stylosanthes and wheat, are sensitive to organic extracts of Cerrado native Annonaceae plants. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 41., 2008, Belo Horizonte. [Resumos...]. Tropical Plant Pathology, v. 33, 2008. Suplemento. Resumo CAL-025. p. S107.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 115. | | SILVA, M. S.; ESPÍNDOLA, L. S.; PAULA, J. E.; ROCHA, T. L.; SILVA, A. C. P.; OLIVEIRA, L. D.; SILVA, L. N.; COSTA, J. V. M.; MURAD, A. M.; CHARCHAR, M. J. d'A.; ANJOS, J. R. N. Fungi, pathogenic to soybean, cotton, stylosanthes and wheat, are sensitive to organic extracts of Cerrado native Annonaceae plants. Tropical Plant Pathology, v. 33, p. S107, 2008. Suplemento. Trabalho apresentado no 41º Congresso Brasileiro de Fitopatologia, 2008, Belo Horizonte.| Biblioteca(s): Embrapa Cerrados. |
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| 116. | | ALMEIDA, C. D. S. de; SPUZA, D. dos S. de L.; DEL SARTO, R. P.; FIRMINO, A. A. P.; SILVA, T. S.; MAGALHÃES, J. C. C.; GROSSI DE SÁ, M. F.; ROCHA, T. L. Fracionamento de extrato aquoso de sementes de Crotalaria espectabilis efetivo no controle de juvenis de segundo estádio (J2) de Meloidogyne incógnita. Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2008. 11 p. (Embrapa Recursos Genéticos e Biotecnologia. Circular Técnica, 78).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 117. | | GROSSI de SÁ, M.; SOUZA, D. S. L.; FRAGOSO, R. da R.; PINTO, E. R. de C.; ROCHA, T. L.; BARBOSA, A. E. A. D.; SA, M. F. G. de. Functional analysis of genes involved with phytoparasitism of meloidogyne incognita. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programas e resumos... [Brasília, DF]: Sociedade Brasileira de Biotecnologia, 2010. p. 271. p. 271| Biblioteca(s): Embrapa Cerrados. |
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| 118. | | SILVA, M. C. M. da; TEIXEIRA, F. R.; SARTO, R. P. del; COUTINHO, M. V.; ROCHA, T. L.; OLIVEIRA NETO, O. B. de; PAULA, A. W. de; OLIVEIRA, U. A. de; GROSSI DE SÁ, M. F. Evolução in vitro de moléculas com potencial para uso no controle de insetos de grãos armazenados. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 9., 2008, Campinas. Ciência e tecnologia na cadeia produtiva do feijão. Campinas: Instituto Agronômico, 2008. p. 847-850. (IAC. Documentos, 85).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 119. | | SILVA, M. C. M.; DIAS, G. C.; SILVA, M. S.; LACERDA, A. F.; DEL SARTO, R. P.; ROCHA, T. L.; OLIVEIRA NETO, O. B.; COUTINHO, M. V.; BEZERRA, I. C.; GROSSI DE SÁ, M. F. Geração de biblioteca combinatória de genes de defensinas e identificação de variantes com potencial antimicrobiano contra fungos patogênicos de algodoeiro e soja. In: WORKSHOP INTERAÇÃO MOLECULAR PLANTA-PRAGAS, 2., 2007, Brasília, DF. II Workshop Interação Molecular Planta-Praga. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 16-20. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 229).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 120. | | SILVA, M. C. M.; SILVA, M. S.; LACERDA, A. F.; DEL SARTO, R. P.; ROCHA, T. L.; OLIVEIRA NETO, O. B.; COUTINHO, M. V.; GROSSI-DE-SÁ, M. F. Geração de biblioteca combinatória de genes de defensinas e identificação de variantes com potencial antimicrobiano contra fungos patogênicos de algodoeiro e soja. In: WORKSHOP INTERAÇÃO MOLECULAR PLANTA-PRAGA, 2., 2007, Brasília. Anais... Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 11-15.| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 183 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
13/04/2011 |
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Data da última atualização: |
13/04/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
FRANCO, O. L.; ANDRADE, A. E.; BARROS, E. V. S. A.; SA, M. F. G. de; CARNEIRO, R. M. D. G.; CARNEIRO, R.; EIRA, M. T. S. da; ROCHA, T. L.; REIS, A. M. dos. |
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Afiliação: |
UNIVERSIDADE CATÓLICA DE BRASÍLIA; MARIA FATIMA GROSSI DE SA, CENARGEN; REGINA MARIA DECHECHI G CARNEIRO, CENARGEN; IAPAR; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; THALES LIMA ROCHA, CENARGEN; ANGELA MEHTA DOS REIS, CENARGEN. |
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Título: |
Comparison of different staining methods for coffee proteomic analysis. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
Proteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analysis revealed an enhanced number of protein spots, as well as differentially expressed proteins, when CBB was used. A total of approximately 70 and 100 spots were observed in silver and CBB stained gels, respectively. Moreover, 18 differentially expressed proteins were observed in CBB gels, and only 8 in the silver stained gels. This report showed that the staining method was crucial for an optimized protein analysis of coffee. Similar results were obtained for cotton roots and therefore these results may be extended to other plant species in order to better understand the host-pathogen interaction. MenosProteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analys... Mostrar Tudo |
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Palavras-Chave: |
Bidimensional electrophoresis; Proteomic method. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32592/1/Comparison-of-Different.pdf
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Marc: |
LEADER 02933nam a2200229 a 4500
001 1885752
005 2011-04-13
008 2007 bl uuuu u00u1 u #d
100 1 $aFRANCO, O. L.
245 $aComparison of different staining methods for coffee proteomic analysis.$h[electronic resource]
260 $aIn:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.$c2007
520 $aProteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analysis revealed an enhanced number of protein spots, as well as differentially expressed proteins, when CBB was used. A total of approximately 70 and 100 spots were observed in silver and CBB stained gels, respectively. Moreover, 18 differentially expressed proteins were observed in CBB gels, and only 8 in the silver stained gels. This report showed that the staining method was crucial for an optimized protein analysis of coffee. Similar results were obtained for cotton roots and therefore these results may be extended to other plant species in order to better understand the host-pathogen interaction.
653 $aBidimensional electrophoresis
653 $aProteomic method
700 1 $aANDRADE, A. E.
700 1 $aBARROS, E. V. S. A.
700 1 $aSA, M. F. G. de
700 1 $aCARNEIRO, R. M. D. G.
700 1 $aCARNEIRO, R.
700 1 $aEIRA, M. T. S. da
700 1 $aROCHA, T. L.
700 1 $aREIS, A. M. dos
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