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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/02/2009 |
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Data da última atualização: |
03/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GODOY, L. P.; VASCONCELOS, A. T. R.; CHUEIRE, L. M. O.; SOUZA, R. C.; NICOLÁS, M. F.; BARCELLOS, F. G.; HUNGRIA, M. |
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Afiliação: |
Leandro Pereira Godoy, CNPSo/UEL; Ana Tereza Ribeiro Vasconcelos Laboratório Nacional de Computação Científica Petrópolis RJ; Ligia Maria Oliveira Chueire, CNPSo; Rangel Celso Souza Laboratório Nacional de Computação Científica Petrópolis RJ; Marisa Fabiana Nicolás Laboratório Nacional de Computação Científica Petrópolis RJ; Fernando Gomes Barcellos, CNPS; Mariangela Hungria, CNPSo. |
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Título: |
Genomic panorama of Bradyrhizobium japonicum CPAC 15, a commercial inoculant strain largely established in Brazilian soils and belonging to the same serogroup as USDA 123. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Soil Biology & Biochemistry, v. 40, n. 11, p. 2743-2753, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Of the many genomes of prokaryotes that have been sequenced, most are pathogenic organisms and very few of agriculturally beneficial bacteria. Soybean, the most important cash crop in Brazil, can provide its need for nitrogen through a symbiosis with exotic strains of bradyrhizobia. Bradyrhizobium japonicum strain CPAC 15 (equates to SEMIA 5079, the same serogroup as USDA 123), which is a highly competitive commercial strain applied to soybean crops since the early 1990s, is now established on several millions of hectares. As financial resources for sequencing genomes are still very limited in developing countries, a panoramic genomic view of CPAC 15 was generated. A total of 4328 shotgun reads resulted in 2,046,740 bp with a phred score_ 20; the assemblage resulted in 1106 phrap contigs scattered by 69 scaffolds and 966 isolated contigs, with an average of 2.5 reads per contig, covering approximately 13% of the genome. Annotation identified 1371 coding DNA sequences (CDSs), 53% with putative known functions, 23% encoding conserved hypothetical and 24% hypothetical genes, representing about 16% of the estimated putative genes. Several comparisons - on COG and KEGG databases, tRNAs, transposases, Gþ C content of CPAC 15 with the complete genome of B. japonicum strain USDA 110 indicated a successful coverage of the whole genome. However, the two strains were surprisingly different, as at least 35% of the CDS of CPAC 15 shows higher similarity to microorganisms other than strain USDA 110. Several new putative genes and others with low similarity to USDA 110, were identified. These were related to nodulation, interaction with the host plant and adaptation, e.g., nodB, nodW, ndvA, effector nopP, genes of secretion systems, transporters and environmentally related genes. MenosOf the many genomes of prokaryotes that have been sequenced, most are pathogenic organisms and very few of agriculturally beneficial bacteria. Soybean, the most important cash crop in Brazil, can provide its need for nitrogen through a symbiosis with exotic strains of bradyrhizobia. Bradyrhizobium japonicum strain CPAC 15 (equates to SEMIA 5079, the same serogroup as USDA 123), which is a highly competitive commercial strain applied to soybean crops since the early 1990s, is now established on several millions of hectares. As financial resources for sequencing genomes are still very limited in developing countries, a panoramic genomic view of CPAC 15 was generated. A total of 4328 shotgun reads resulted in 2,046,740 bp with a phred score_ 20; the assemblage resulted in 1106 phrap contigs scattered by 69 scaffolds and 966 isolated contigs, with an average of 2.5 reads per contig, covering approximately 13% of the genome. Annotation identified 1371 coding DNA sequences (CDSs), 53% with putative known functions, 23% encoding conserved hypothetical and 24% hypothetical genes, representing about 16% of the estimated putative genes. Several comparisons - on COG and KEGG databases, tRNAs, transposases, Gþ C content of CPAC 15 with the complete genome of B. japonicum strain USDA 110 indicated a successful coverage of the whole genome. However, the two strains were surprisingly different, as at least 35% of the CDS of CPAC 15 shows higher similarity to microorganisms other than strai... Mostrar Tudo |
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Thesagro: |
Solo. |
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Categoria do assunto: |
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Marc: |
LEADER 02467naa a2200205 a 4500
001 1469733
005 2017-08-03
008 2008 bl --- 0-- u #d
100 1 $aGODOY, L. P.
245 $aGenomic panorama of Bradyrhizobium japonicum CPAC 15, a commercial inoculant strain largely established in Brazilian soils and belonging to the same serogroup as USDA 123.
260 $c2008
520 $aOf the many genomes of prokaryotes that have been sequenced, most are pathogenic organisms and very few of agriculturally beneficial bacteria. Soybean, the most important cash crop in Brazil, can provide its need for nitrogen through a symbiosis with exotic strains of bradyrhizobia. Bradyrhizobium japonicum strain CPAC 15 (equates to SEMIA 5079, the same serogroup as USDA 123), which is a highly competitive commercial strain applied to soybean crops since the early 1990s, is now established on several millions of hectares. As financial resources for sequencing genomes are still very limited in developing countries, a panoramic genomic view of CPAC 15 was generated. A total of 4328 shotgun reads resulted in 2,046,740 bp with a phred score_ 20; the assemblage resulted in 1106 phrap contigs scattered by 69 scaffolds and 966 isolated contigs, with an average of 2.5 reads per contig, covering approximately 13% of the genome. Annotation identified 1371 coding DNA sequences (CDSs), 53% with putative known functions, 23% encoding conserved hypothetical and 24% hypothetical genes, representing about 16% of the estimated putative genes. Several comparisons - on COG and KEGG databases, tRNAs, transposases, Gþ C content of CPAC 15 with the complete genome of B. japonicum strain USDA 110 indicated a successful coverage of the whole genome. However, the two strains were surprisingly different, as at least 35% of the CDS of CPAC 15 shows higher similarity to microorganisms other than strain USDA 110. Several new putative genes and others with low similarity to USDA 110, were identified. These were related to nodulation, interaction with the host plant and adaptation, e.g., nodB, nodW, ndvA, effector nopP, genes of secretion systems, transporters and environmentally related genes.
650 $aSolo
700 1 $aVASCONCELOS, A. T. R.
700 1 $aCHUEIRE, L. M. O.
700 1 $aSOUZA, R. C.
700 1 $aNICOLÁS, M. F.
700 1 $aBARCELLOS, F. G.
700 1 $aHUNGRIA, M.
773 $tSoil Biology & Biochemistry$gv. 40, n. 11, p. 2743-2753, 2008.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
24/01/2014 |
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Data da última atualização: |
31/08/2021 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ROSA-CAMPOS, A. A.; ROCHA, J. E. da S.; BORGO, L. A. |
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Afiliação: |
RAFAEL STORTO NALIN, Pós-graduação - Universidade de Brasília (UnB) - Brasília, DF, Brasil; JULIANA EVANGELISTA DA SILVA ROCHA, CNPC; LUIZ ANTÔNIO BORGO, UFLA - Lavras, MG, Brasil. |
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Título: |
Avaliação qualitativa e quantitativa de leite Tipo A comercializados no Distrito Federal. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 8., 2013, Fortaleza. [Anais...]. [Sobral: Universidade Estadual Vale do Acaraú; Embrapa Caprinos e Ovinos, 2013]. 3 f. |
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Idioma: |
Português |
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Conteúdo: |
Resumo: O leite tipo A é processado em granjas leiteiras. Possui maior valor agregado e menor carga microbiológica. O objetivo desse trabalho foi avaliar a qualidade do produto comercializado no Distrito Federal. Foram avaliadas duas marcas durante quatro meses. As amostras foram avaliadas em testes de fraude e foram feitas análise físico-química. Ambas as marcas apresentaram índice crioscópico em desacordo com a legislação vigente e presença de sacarose. Esse resultado evidencia a fraude para aumentar o rendimento do produto. Além disso, a marca B apresentou bicarbonato em sua composição e teste de peroxidase negativa em 14,3% das amostras. Esses resultados mostram que a marca B fraudou o leite na tentativa de reduzir a carga microbiana. [Qualitative and Quantitative Evaluation of type A milk marketed in the Federal District]. Abstract: The type A milk is processed into dairy farms. Has a higher value and lower microbiological load. The aim of this study was to evaluate the quality of the product marketed in the Federal District. We evaluated two brands during four months. The samples were evaluated in tests of fraud and physicochemical analysis. Both brands had cryoscopic index in disagreement with current legislation and presence of sucrose. This result evidences the fraud to increase product yield. In addition, the type B showed bicarbonate in their composition and peroxidase negative test in 14,3% of samples. These results show that type B defrauded milk in attempt to reduce the microbial load. MenosResumo: O leite tipo A é processado em granjas leiteiras. Possui maior valor agregado e menor carga microbiológica. O objetivo desse trabalho foi avaliar a qualidade do produto comercializado no Distrito Federal. Foram avaliadas duas marcas durante quatro meses. As amostras foram avaliadas em testes de fraude e foram feitas análise físico-química. Ambas as marcas apresentaram índice crioscópico em desacordo com a legislação vigente e presença de sacarose. Esse resultado evidencia a fraude para aumentar o rendimento do produto. Além disso, a marca B apresentou bicarbonato em sua composição e teste de peroxidase negativa em 14,3% das amostras. Esses resultados mostram que a marca B fraudou o leite na tentativa de reduzir a carga microbiana. [Qualitative and Quantitative Evaluation of type A milk marketed in the Federal District]. Abstract: The type A milk is processed into dairy farms. Has a higher value and lower microbiological load. The aim of this study was to evaluate the quality of the product marketed in the Federal District. We evaluated two brands during four months. The samples were evaluated in tests of fraud and physicochemical analysis. Both brands had cryoscopic index in disagreement with current legislation and presence of sucrose. This result evidences the fraud to increase product yield. In addition, the type B showed bicarbonate in their composition and peroxidase negative test in 14,3% of samples. These results show that type B defrauded milk in attempt to r... Mostrar Tudo |
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Palavras-Chave: |
Análise físico-química; Chemicophysical properties; Delinquent behaviour; Fraude do leite; Leite tipo A. |
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Thesagro: |
Adulteração; Análise microbiológica; Leite pasteurizado; Microbiologia de alimento; Qualidade. |
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Thesaurus NAL: |
Brazil; Food quality; Microbiology; Pasteurized milk. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/95799/1/aac-Avaliacao-qualitativa-e-quantitativa.pdf
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Marc: |
LEADER 02566nam a2200301 a 4500
001 1977391
005 2021-08-31
008 2013 bl uuuu u00u1 u #d
100 1 $aROSA-CAMPOS, A. A.
245 $aAvaliação qualitativa e quantitativa de leite Tipo A comercializados no Distrito Federal.$h[electronic resource]
260 $aIn: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 8., 2013, Fortaleza. [Anais...]. [Sobral: Universidade Estadual Vale do Acaraú; Embrapa Caprinos e Ovinos, 2013]. 3 f.$c2013
520 $aResumo: O leite tipo A é processado em granjas leiteiras. Possui maior valor agregado e menor carga microbiológica. O objetivo desse trabalho foi avaliar a qualidade do produto comercializado no Distrito Federal. Foram avaliadas duas marcas durante quatro meses. As amostras foram avaliadas em testes de fraude e foram feitas análise físico-química. Ambas as marcas apresentaram índice crioscópico em desacordo com a legislação vigente e presença de sacarose. Esse resultado evidencia a fraude para aumentar o rendimento do produto. Além disso, a marca B apresentou bicarbonato em sua composição e teste de peroxidase negativa em 14,3% das amostras. Esses resultados mostram que a marca B fraudou o leite na tentativa de reduzir a carga microbiana. [Qualitative and Quantitative Evaluation of type A milk marketed in the Federal District]. Abstract: The type A milk is processed into dairy farms. Has a higher value and lower microbiological load. The aim of this study was to evaluate the quality of the product marketed in the Federal District. We evaluated two brands during four months. The samples were evaluated in tests of fraud and physicochemical analysis. Both brands had cryoscopic index in disagreement with current legislation and presence of sucrose. This result evidences the fraud to increase product yield. In addition, the type B showed bicarbonate in their composition and peroxidase negative test in 14,3% of samples. These results show that type B defrauded milk in attempt to reduce the microbial load.
650 $aBrazil
650 $aFood quality
650 $aMicrobiology
650 $aPasteurized milk
650 $aAdulteração
650 $aAnálise microbiológica
650 $aLeite pasteurizado
650 $aMicrobiologia de alimento
650 $aQualidade
653 $aAnálise físico-química
653 $aChemicophysical properties
653 $aDelinquent behaviour
653 $aFraude do leite
653 $aLeite tipo A
700 1 $aROCHA, J. E. da S.
700 1 $aBORGO, L. A.
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