Data/hora: 09/06/2024 05:04:36

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	12/12/2017	
Data da última atualização: 	10/08/2022	
Autoria: 	SIQUEIRA, L. G. B.; TRIBULO, P.; CHEN, Z.; DENICOL, A. C.; ORTEGA, M. S.; NEGRON-PEREZ, V.M.; KANNAMPUZHA-FRANCIS, J. P.; POHLER, K. G.; RIVERA, R. M.; HANSEN, P. J.	
Afiliação: 	LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; P. TRIBULO, UNIVERSITY OF FLORIDA/USA; Zhiyuan Chen, University of Missouri, Columbia, MO; A. C. DENICOL, UNIVERSITY OF FLORIDA/USA; M. S. ORTEGA, UNIVERSITY OF FLORIDA/USA; V. M. NEGRON-PEREZ, UNIVERSITY OF FLORIDA/USA; J. P. KANNAMPUZHA-FRANCIS, UNIVERSITY OF FLORIDA/USA; Ky G. Pohler, University of Tennessee, Knoxville, TN; Rocio M. Rivera, University of Missouri, Columbia, MO; P. J. HANSEN, UNIVERSITY OF FLORIDA/USA.	
Título: 	Colony-stimulating factor 2 acts from days 5 to 7 of development to modify programming of the bovine conceptus at day 86 of gestation.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Biology of Reproduction, v. 96, n. 4, p. 743-757, 2017.	
DOI: 	http://doi.org/10.1093/biolre/iox018	
Idioma: 	Inglês	
Conteúdo: 	Colony-stimulating factor 2 (CSF2) is an embryokine that improves competence of the embryo to establish pregnancy and which may participate in developmental programming. We tested whether culture of bovine embryos with CSF2 alters fetal development and alleviates abnormalities associated with in vitro production (IVP) of embryos. Pregnancies were established by artificial insemination (AI), transfer of an IVP embryo (IVP) or transfer of an IVP embryo treated with 10 ng/ml CSF2 from day 5 to 7 of development (CSF2). Pregnancies were produced using X-sorted semen from a single sire. Female singleton conceptuses were collected on day 86 of gestation. There were few morphological differences between groups although IVP and CSF2 fetuses were heavier than AI fetuses. Bicarbonate concentration in allantoic fluid was lower for IVP than for AI or CSF2. Expression of 92 genes in liver, placenta, and muscle was determined by RT-qPCR. The general pattern for liver and placenta was for IVP to alter expression and for CSF2 to sometimes reverse this effect. For muscle, CSF2 affected gene expression but did not generally reverse effects of IVP. Levels of methylation for each of the three tissues at 12 loci in the promoter of IGF2 and five in the promoter of GRB10 were unaffected by treatment except for CSF2 effects on two CpG for IGF2 in placenta and muscle. In conclusion, CSF2 can act as a developmental programming agent but alone is not able to abolish the adverse effects of in vitro production on fetal characteristics.	
Palavras-Chave: 	Colony-stimulating factor 2; Embryokine; Programming.	
Thesaurus Nal: 	fetal development.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (23769-1) - UPC - AP - DD -  -  -  - DIS