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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
10/12/2015 |
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Data da última atualização: |
26/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ABREU, E. F. M.; DALTRO, C. B.; NOGUEIRA, E. O. P. L.; ANDRADE, E. C.; ARAGAO, F. J. L. |
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Afiliação: |
EMANUEL FELIPE MEDEIROS ABREU, CNPMF; CLEIDIANE B. DALTRO; ELSA OLIVEIRA P E LAGO NOGUEIRA, CENARGEN; EDUARDO C. ANDRADE, CNPMF; FRANCISCO JOSE LIMA ARAGAO, CENARGEN. |
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Título: |
Sequence and genome organization of papaya meleira virus infecting papaya in Brazil. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Archives of Virology, v. 160, n. 12, p. 3143-3147, 2015. |
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DOI: |
10.1007/s00705-015-2605-x |
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Idioma: |
Inglês |
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Conteúdo: |
Papaya sticky disease ('meleira') was first observed in Brazil at the beginning of the 1980s. The disease is characterized by intense latex exudation from the fruit surface that becomes dark as it oxidizes, which makes it difficult to sell. The causal agent, which has been called papaya meleira virus (PMeV), has been identified as an isometric virus particle, approximately 50 nm in diameter, with a double-stranded RNA genome. Here, we report the first complete sequence and organization of the 8.7-kb viral dsRNA genome. Two ORFs coding for a putative coat protein and RNA-dependent RNA polymerase (RdRp) were predicted. In silico analysis revealed that the translated ORF2 contains the conserved domains characteristic of an RdRp protein (pfam02123:RdRP 4), which is a family that includes RdRps from members of the genera Luteovirus, Totivirus and Rotavirus. Evolutionary analysis with amino acid sequences with the RdRps from members of the family Totiviridae and some dsRNA viruses showed that PMeV RdRp did not root itself in any genus. |
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Palavras-Chave: |
Meleira; Papaya sticky disease. |
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Thesagro: |
Mamão. |
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Thesaurus Nal: |
Papayas. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182271/1/Abreu2015-Article-SequenceAndGenomeOrganizationO.pdf
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Marc: |
LEADER 01722naa a2200229 a 4500
001 2031416
005 2024-04-26
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1007/s00705-015-2605-x$2DOI
100 1 $aABREU, E. F. M.
245 $aSequence and genome organization of papaya meleira virus infecting papaya in Brazil.$h[electronic resource]
260 $c2015
520 $aPapaya sticky disease ('meleira') was first observed in Brazil at the beginning of the 1980s. The disease is characterized by intense latex exudation from the fruit surface that becomes dark as it oxidizes, which makes it difficult to sell. The causal agent, which has been called papaya meleira virus (PMeV), has been identified as an isometric virus particle, approximately 50 nm in diameter, with a double-stranded RNA genome. Here, we report the first complete sequence and organization of the 8.7-kb viral dsRNA genome. Two ORFs coding for a putative coat protein and RNA-dependent RNA polymerase (RdRp) were predicted. In silico analysis revealed that the translated ORF2 contains the conserved domains characteristic of an RdRp protein (pfam02123:RdRP 4), which is a family that includes RdRps from members of the genera Luteovirus, Totivirus and Rotavirus. Evolutionary analysis with amino acid sequences with the RdRps from members of the family Totiviridae and some dsRNA viruses showed that PMeV RdRp did not root itself in any genus.
650 $aPapayas
650 $aMamão
653 $aMeleira
653 $aPapaya sticky disease
700 1 $aDALTRO, C. B.
700 1 $aNOGUEIRA, E. O. P. L.
700 1 $aANDRADE, E. C.
700 1 $aARAGAO, F. J. L.
773 $tArchives of Virology$gv. 160, n. 12, p. 3143-3147, 2015.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
15/03/1999 |
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Data da última atualização: |
15/03/1999 |
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Autoria: |
RIBEIRO, J. R. A.; OLIVEIRA, A. L. B.; RUMJANEK, V.; RUMJANEK, Norma G. |
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Título: |
Serological recognition of polysaccharides from Bradyrhizobium. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 26., maio 1997, Caxambu. Resumos... Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1997. p.135. |
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Idioma: |
Inglês |
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Conteúdo: |
Rhizobia are gram-negative bacteria which have on their surface a high amount of polysaccharides. The more important sugars are the exopolysaccharides (EPS), capsular polysaccharides (CPS) and lipopolysaccharides (LPS). Rhizobia are symbiotic nitrogen-fixing bacteria which infect the roots of leguminous plants. Bacterial competition, infection of the plant by the bacteria, nodule formation and fixation efficiency are processes that depend heavily on the intense exchange of molecular signals between symbionts and the polysaccharides are believed to play a major part in this. Serological techniques have been used extensively in rhizobia identification. Although these are limited to those strains that crossreact within a given serogroup, common serological reactions indicate, in general, a certain degree of surface similarity which may be related to physiological characteristics. Serology can also be used in competition assays and for identification of bacteria inside the leguminous root nodule. In this study, we investigated the possibility of the polysaccharide being recognized by the antiserum prepared with whole cells wich are commonly used to identify bacteria and also compare the response of bacteria and polysaccharide. The two antisera (antiBR-33 strain of B. japonicum and antiBR-96 strain of B. japonicum and antiBR-96 strain of B. elkanii) were allowed to react with homologous and heterologous polysaccharides. The homologous recognition was at least 100 times stronger than the heterologous one. Both... MenosRhizobia are gram-negative bacteria which have on their surface a high amount of polysaccharides. The more important sugars are the exopolysaccharides (EPS), capsular polysaccharides (CPS) and lipopolysaccharides (LPS). Rhizobia are symbiotic nitrogen-fixing bacteria which infect the roots of leguminous plants. Bacterial competition, infection of the plant by the bacteria, nodule formation and fixation efficiency are processes that depend heavily on the intense exchange of molecular signals between symbionts and the polysaccharides are believed to play a major part in this. Serological techniques have been used extensively in rhizobia identification. Although these are limited to those strains that crossreact within a given serogroup, common serological reactions indicate, in general, a certain degree of surface similarity which may be related to physiological characteristics. Serology can also be used in competition assays and for identification of bacteria inside the leguminous root nodule. In this study, we investigated the possibility of the polysaccharide being recognized by the antiserum prepared with whole cells wich are commonly used to identify bacteria and also compare the response of bacteria and polysaccharide. The two antisera (antiBR-33 strain of B. japonicum and antiBR-96 strain of B. japonicum and antiBR-96 strain of B. elkanii) were allowed to react with homologous and heterologous polysaccharides. The homologous recognition was at least 100 times stronger ... Mostrar Tudo |
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Palavras-Chave: |
Bacterias; Methods. |
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Thesagro: |
Bactéria; Imunologia; Método; Polissacarídeo. |
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Thesaurus NAL: |
Bradyrhizobium; immunology; polysaccharides. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02364naa a2200265 a 4500
001 1620980
005 1999-03-15
008 1997 bl uuuu u00u1 u #d
100 1 $aRIBEIRO, J. R. A.
245 $aSerological recognition of polysaccharides from Bradyrhizobium.
260 $c1997
520 $aRhizobia are gram-negative bacteria which have on their surface a high amount of polysaccharides. The more important sugars are the exopolysaccharides (EPS), capsular polysaccharides (CPS) and lipopolysaccharides (LPS). Rhizobia are symbiotic nitrogen-fixing bacteria which infect the roots of leguminous plants. Bacterial competition, infection of the plant by the bacteria, nodule formation and fixation efficiency are processes that depend heavily on the intense exchange of molecular signals between symbionts and the polysaccharides are believed to play a major part in this. Serological techniques have been used extensively in rhizobia identification. Although these are limited to those strains that crossreact within a given serogroup, common serological reactions indicate, in general, a certain degree of surface similarity which may be related to physiological characteristics. Serology can also be used in competition assays and for identification of bacteria inside the leguminous root nodule. In this study, we investigated the possibility of the polysaccharide being recognized by the antiserum prepared with whole cells wich are commonly used to identify bacteria and also compare the response of bacteria and polysaccharide. The two antisera (antiBR-33 strain of B. japonicum and antiBR-96 strain of B. japonicum and antiBR-96 strain of B. elkanii) were allowed to react with homologous and heterologous polysaccharides. The homologous recognition was at least 100 times stronger than the heterologous one. Both...
650 $aBradyrhizobium
650 $aimmunology
650 $apolysaccharides
650 $aBactéria
650 $aImunologia
650 $aMétodo
650 $aPolissacarídeo
653 $aBacterias
653 $aMethods
700 1 $aOLIVEIRA, A. L. B.
700 1 $aRUMJANEK, V.
700 1 $aRUMJANEK, Norma G.
773 $tIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 26., maio 1997, Caxambu. Resumos... Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1997. p.135.
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Embrapa Agrobiologia (CNPAB) |
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