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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
05/02/2018 |
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Data da última atualização: |
06/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, A. S.; SEYFRIED, M.; CAMPESTRINI, L. H.; BAGGIO, S. F. Z.; MINHO, A. P.; MOLENTO, M. B.; MAURER, J. B. B. |
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Afiliação: |
Andressa Soldera Silva, UFPR; Melina Seyfried, UFPR; Luciano Henrique Campestrini, UFPR; Selma Faria Zawadzki Baggio, UFPR; ALESSANDRO PELEGRINE MINHO, CPPSE; Marcelo Beltrão Molento, UFPR; Juliana Bello Baron Maurer, UFPR. |
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Título: |
Assessment of anthelmintic activity and bio-guided chemical analysis of Persea americana seed extracts. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 251, p. 34-43, feb. 2018. |
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DOI: |
10.1016/j.vetpar.2017.12.019 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to characterize the extracts and fractions of Persea americana Mill. (Avocado) seeds and to determine the composition and the in vitro anthelmintic activity against third-stage larvae (L3) of Haemonchus contortus. The fresh (F) and dried (H) avocado seeds (PA) were subjected to extraction with hot water (W-PAF, W-PAH), ethanol (E-PAF, E-PAH) or methanol 70% (v/v), and partition with solvents of increasing polarity [n-hexane (H-PAF, H-PAH), chloroform (C-PAF, C-PAH), ethyl acetate (Ea-PAF, Ea-PAH), and n-butanol (B-PAF, B-PAH)], yielding a total of 14 extracts/fractions. After considering the yield, water solubility, and the preliminary results of the larval migration test (LMT), the E-PAF, E-PAH, H-PAF, and H-PAH were selected for further experiments. E-PAH presented an efficiency concentration of 50% (EC50) of 36 μg/mL on the LMT. E-PAH showed the greatest efficiency when its EC50 was compared to the other fractions (E-PAF = 147 μg/mL; H-PAF = 801 μg/mL; H-PAH = 77 μg/mL). After that, the E-PAH was chemically characterized, considering its quantitative polyphenolic and flavonoid contents by colorimetric and chromatographic techniques. E-PAH presented 50, 38, and 24 mg/g of dry matter of total phenol, condensed tannins (CT), and flavonoid contents, respectively. Using high performance liquid chromatography (HPLC) analysis, E-PAH had shown to have epicatechin (4.7 μg/mL), rutin (2.8 μg/mL), and chlorogenic acid (1.4 μg/mL) as its main constituents besides quercetin. These isolated compounds were evaluated using the LMT in order to relate the composition to the anthelmintic activity observed for E-PAH. Quercetin (EC50 = 7.8 μg/mL) and epicatechin (EC50 = 10 μg/mL) presented a higher efficiency than rutin (EC50 = 30 μg/mL). Chlorogenic acid was also tested with the LMT but did not present a significant efficiency. According to the results, the phenolic composition of E-PAH and the EC50 values obtained for the isolated phenols, it can be suggested that, besides the CT content, the presence of epicatechin and rutin contributed to the larvicidal activity of E-PAH. In conclusion, avocado seeds may be used as a source of polyphenols with promising anthelmintic applications. MenosThe aim of this study was to characterize the extracts and fractions of Persea americana Mill. (Avocado) seeds and to determine the composition and the in vitro anthelmintic activity against third-stage larvae (L3) of Haemonchus contortus. The fresh (F) and dried (H) avocado seeds (PA) were subjected to extraction with hot water (W-PAF, W-PAH), ethanol (E-PAF, E-PAH) or methanol 70% (v/v), and partition with solvents of increasing polarity [n-hexane (H-PAF, H-PAH), chloroform (C-PAF, C-PAH), ethyl acetate (Ea-PAF, Ea-PAH), and n-butanol (B-PAF, B-PAH)], yielding a total of 14 extracts/fractions. After considering the yield, water solubility, and the preliminary results of the larval migration test (LMT), the E-PAF, E-PAH, H-PAF, and H-PAH were selected for further experiments. E-PAH presented an efficiency concentration of 50% (EC50) of 36 μg/mL on the LMT. E-PAH showed the greatest efficiency when its EC50 was compared to the other fractions (E-PAF = 147 μg/mL; H-PAF = 801 μg/mL; H-PAH = 77 μg/mL). After that, the E-PAH was chemically characterized, considering its quantitative polyphenolic and flavonoid contents by colorimetric and chromatographic techniques. E-PAH presented 50, 38, and 24 mg/g of dry matter of total phenol, condensed tannins (CT), and flavonoid contents, respectively. Using high performance liquid chromatography (HPLC) analysis, E-PAH had shown to have epicatechin (4.7&#... Mostrar Tudo |
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Palavras-Chave: |
Avocado. |
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Thesagro: |
Haemonchus Contortus. |
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Thesaurus Nal: |
epicatechin. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03149naa a2200241 a 4500
001 2087019
005 2018-12-06
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.vetpar.2017.12.019$2DOI
100 1 $aSILVA, A. S.
245 $aAssessment of anthelmintic activity and bio-guided chemical analysis of Persea americana seed extracts.$h[electronic resource]
260 $c2018
520 $aThe aim of this study was to characterize the extracts and fractions of Persea americana Mill. (Avocado) seeds and to determine the composition and the in vitro anthelmintic activity against third-stage larvae (L3) of Haemonchus contortus. The fresh (F) and dried (H) avocado seeds (PA) were subjected to extraction with hot water (W-PAF, W-PAH), ethanol (E-PAF, E-PAH) or methanol 70% (v/v), and partition with solvents of increasing polarity [n-hexane (H-PAF, H-PAH), chloroform (C-PAF, C-PAH), ethyl acetate (Ea-PAF, Ea-PAH), and n-butanol (B-PAF, B-PAH)], yielding a total of 14 extracts/fractions. After considering the yield, water solubility, and the preliminary results of the larval migration test (LMT), the E-PAF, E-PAH, H-PAF, and H-PAH were selected for further experiments. E-PAH presented an efficiency concentration of 50% (EC50) of 36 μg/mL on the LMT. E-PAH showed the greatest efficiency when its EC50 was compared to the other fractions (E-PAF = 147 μg/mL; H-PAF = 801 μg/mL; H-PAH = 77 μg/mL). After that, the E-PAH was chemically characterized, considering its quantitative polyphenolic and flavonoid contents by colorimetric and chromatographic techniques. E-PAH presented 50, 38, and 24 mg/g of dry matter of total phenol, condensed tannins (CT), and flavonoid contents, respectively. Using high performance liquid chromatography (HPLC) analysis, E-PAH had shown to have epicatechin (4.7 μg/mL), rutin (2.8 μg/mL), and chlorogenic acid (1.4 μg/mL) as its main constituents besides quercetin. These isolated compounds were evaluated using the LMT in order to relate the composition to the anthelmintic activity observed for E-PAH. Quercetin (EC50 = 7.8 μg/mL) and epicatechin (EC50 = 10 μg/mL) presented a higher efficiency than rutin (EC50 = 30 μg/mL). Chlorogenic acid was also tested with the LMT but did not present a significant efficiency. According to the results, the phenolic composition of E-PAH and the EC50 values obtained for the isolated phenols, it can be suggested that, besides the CT content, the presence of epicatechin and rutin contributed to the larvicidal activity of E-PAH. In conclusion, avocado seeds may be used as a source of polyphenols with promising anthelmintic applications.
650 $aepicatechin
650 $aHaemonchus Contortus
653 $aAvocado
700 1 $aSEYFRIED, M.
700 1 $aCAMPESTRINI, L. H.
700 1 $aBAGGIO, S. F. Z.
700 1 $aMINHO, A. P.
700 1 $aMOLENTO, M. B.
700 1 $aMAURER, J. B. B.
773 $tVeterinary Parasitology$gv. 251, p. 34-43, feb. 2018.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
19/11/1996 |
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Data da última atualização: |
13/08/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
VIEIRA, L. da S.; LIMA, J. D.; RIBEIRO, M. F. B.; BOZZI, I. A.; CAMARGOS, E. R. S. |
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Afiliação: |
LUIZ DA SILVA VIEIRA, CNPC. |
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Título: |
Ultrastructure of endogenous stages of Eimeria ninakohlyakimovae Yakimov & Rategaieff, 1930 Emend. Levine, 1961 in experimentally infected goat. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
In: CONGRESSO PANAMERICANO DE CIÊNCIAS VETERINÁRIAS, 15., 1996, Campo Grande. Resumos... Campo Grande: Associacao Panamericana de Ciencias Veterinarias, 1996. p. 341. Ref. 242. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Eimeria sp; Eimeria spp; Experimental infection; Infecção experimental; Ultra-estrutura. |
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Thesagro: |
Caprino. |
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Thesaurus NAL: |
Goats; Ultrastructure. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 00909nam a2200241 a 4500
001 1514656
005 2021-08-13
008 1996 bl uuuu u01u1 u #d
100 1 $aVIEIRA, L. da S.
245 $aUltrastructure of endogenous stages of Eimeria ninakohlyakimovae Yakimov & Rategaieff, 1930 Emend. Levine, 1961 in experimentally infected goat.$h[electronic resource]
260 $aIn: CONGRESSO PANAMERICANO DE CIÊNCIAS VETERINÁRIAS, 15., 1996, Campo Grande. Resumos... Campo Grande: Associacao Panamericana de Ciencias Veterinarias, 1996. p. 341. Ref. 242.$c1996
650 $aGoats
650 $aUltrastructure
650 $aCaprino
653 $aEimeria sp
653 $aEimeria spp
653 $aExperimental infection
653 $aInfecção experimental
653 $aUltra-estrutura
700 1 $aLIMA, J. D.
700 1 $aRIBEIRO, M. F. B.
700 1 $aBOZZI, I. A.
700 1 $aCAMARGOS, E. R. S.
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Embrapa Caprinos e Ovinos (CNPC) |
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