Data/hora: 19/06/2024 21:35:11

Biblioteca(s): | Embrapa Pantanal.
Data corrente: | 16/04/2013
Data da última atualização: | 06/08/2013
Autoria: | SILVEIRA, J. A. G.; RABELO, E. M. L.; LACERDA, A. C. R.; BORGES, P. A. L.; TOMAS, W. M.; PELLEGRIN, A. O.; TOMICH, R. G. P.; RIBEIRO, M. F. B.
Afiliação: | JÚLIA A. G. SILVEIRA, UFMG; ÉLIDA M. L. RABELO, UFMG; ANA C. R. LACERDA, Embrapa Pantanal; PAULO A. L. BORGES, Embrapa Pantanal; WALFRIDO MORAES TOMAS, CPAP; AIESCA OLIVEIRA PELLEGRIN, CPAP; RENATA R. G. TOMICH, Embrapa Pantanal; MÚCIO F. B. RIBEIRO, UFMG.
Título: | Molecular detection and identification of hemoparasites in pampas deer (Ozotoceros bezoarticus Linnaeus, 1758) from the Pantanal Brazil.
Ano de publicação: | 2013
Fonte/Imprenta: | Ticks and Tick-borne Diseases, v. 4, n. 4, p. 341-345, 2013.
ISSN: | 1877-959X
Idioma: | Inglês

Conteúdo:

Hemoparasites were surveyed in 60 free-living pampas deer Ozotoceros bezoarticus from the central area of the Pantanal, known as Nhecolândia, State of Mato Grosso do Sul, Brazil, through the analysis of nested PCR assays and nucleotide sequencing. Blood samples were tested for Babesia/Theileria, Anaplasma spp., and Trypanosoma spp. using nPCR assays and sequencing of the 18S rRNA, msp4, ITS, and cathepsin L genes. The identity of each sequence was confirmed by comparison with sequences from GenBank using BLAST software. Forty-six (77%) pampas deer were positive for at least one hemoparasite, according to PCR assays. Co-infection occurred in 13 (22%) animals. Based on the sequencing results, 29 (48%) tested positive for A. marginale. Babesia/Theileria were detected in 23 (38%) samples, and according to the sequencing results 52% (12/23) of the samples were similar to T. cervi, 13% (3/23) were similar to Babesia bovis, and 9% (2/23) were similar to B. bigemina. No samples were amplified with the primers for T. vivax, while 11 (18%) were amplified with the ITS primers for T. evansi. The results showed pampas deer to be co-infected with several hemoparasites, including species that may cause serious disease in cattle. Pampas deer is an endangered species in Brazil, and the consequences of these infections to their
health are poorly understood.

Palavras-Chave: | Brazilian deer; Hemoparasites; Molecular detection.
Thesagro: | Ozotoceros Bezoarticus.
Categoria do assunto: | --
Registro original: | Embrapa Pantanal (CPAP)
Exemplares: | CPAP - (58704-1) - UPC - SP - PP - SP17736 -  - 17736 - DIS