Data/hora: 26/09/2024 12:47:04

Biblioteca(s): 	Embrapa Cerrados; Embrapa Gado de Corte; Embrapa Recursos Genéticos e Biotecnologia.	
Data corrente: 	20/09/2016	
Data da última atualização: 	25/04/2024	
Autoria: 	VILLETH, G. R. C.; CARMO, L. S. T.; SILVA, L. P.; SANTOS, M. F.; OLIVEIRA NETO, O. B. de; GROSSI DE SA, M. F.; RIBEIRO, I. S.; DESSAUNE, S. N.; FRAGOSO, R. R.; FRANCO, O. L.; MEHTA, A.	
Afiliação: 	GABRIELA R. C. VILLETH; LÍLIAN S. T. CARMO; LUCIANO PAULINO DA SILVA, CENARGEN; MATEUS FIGUEIREDO SANTOS, CNPGC; OSMUNDO BRILHANTE DE OLIVEIRA NETO; MARIA FATIMA GROSSI DE SA, CENARGEN; IGOR SOUSA RIBEIRO; SUELEN NOGUEIRA DESSAUNE TAMEIRAO, CPAC; RODRIGO DA ROCHA FRAGOSO, CPAC; OCTÁVIO L. FRANCO, UCB; ANGELA MEHTA DOS REIS, CENARGEN.	
Título: 	Identification of proteins in susceptible and resistant Brassica oleracea responsive to Xanthomonas campestris pv. campestris infection.	
Ano de publicação: 	2016	
Fonte/Imprenta: 	Journal of Proteomics, v. 143, p. 278-285, 2016.	
Idioma: 	Inglês	
Conteúdo: 	Cruciferous plants are important edible vegetables widely consumed around theworld, including cabbage, cauliflower and broccoli. The main disease that affects crucifer plants is black rot, caused by Xanthomonas campestris pv. campestris (Xcc). In order to better understand this specific plant–pathogen interaction, proteins responsive to Xcc infection in resistant (União) and susceptible (Kenzan) Brassica oleracea cultivars were investigated by 2-DE followed by mass spectrometry. A total of 47 variable spots were identified and revealed that in the susceptible interaction there is a clear reduction in the abundance of proteins involved in energetic metabolism and defense. It was interesting to observe that in the resistant interaction, these proteins showed an opposite behavior. Based on our results,we conclude that resistance is correlated with the ability of the plant to keep sufficient photosynthesis metabolismactivity to provide energy supplies necessary for an active defense. As a follow-up study, qRT-PCR analysis of selected genes was performed and revealed that most genes showed an up-regulation trend from 5 to 15 days after inoculation (DAI), showing highest transcript levels at 15 DAI. These results revealed the gradual accumulation of transcripts providing a more detailed view of the changes occurring during different stages of the plant–pathogen interaction. Biological significance: In this study we have compared cultivars of Brassica oleracea (cabbage), susceptible and resistant to black rot, by using the classical 2-DE approach. We have found that resistance is correlated with the ability of the plant to keep sufficient photosynthesismetabolismactivity to provide energy supplies necessary for an active defense.	
Palavras-Chave: 	Black rot; Interação planta patógeno; Plant-pathogen interaction; Proteoma; QRT-PCR.	
Thesagro: 	Podridão Negra.	
Thesaurus Nal: 	proteome.	
Categoria do assunto: 	--
G Melhoramento Genético	
Registro original: 	Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)	
Exemplares: 	CENARGEN - (36384-1) - UPC - AP - DD -  -  -  - DIS
CPAC - (35755-1) - UPC - AP - PP - S2220 -  - S2220 - DIS
CNPGC - (16762-1) - UPC - AP - DD -  -  -  - DIS