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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Pecuária Sudeste. |
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Data corrente: |
25/08/2017 |
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Data da última atualização: |
25/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CASTRO, L. M. de; ROSA, G. J. de M.; LOPES, F. B.; REGITANO, L. C. de A.; ROSA, A. J. de M.; MAGNABOSCO, C. de U. |
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Afiliação: |
Letícia Mendes de Castro, UFG; Guilherme Jordão de Magalhães Rosa, University of Wisconsin; Fernando Brito Lopes, Bolsista/Embrapa Cerrados; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; ARTUR JORDAO DE MAGALHAES ROSA, CPAC; CLAUDIO DE ULHOA MAGNABOSCO, CPAC. |
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Título: |
Genomewide association mapping and pathway analysis of meat tenderness in Polled Nellore cattle. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Animal Science, v. 95, n. 5, p. 1945-1956, 2017. |
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DOI: |
10.2527/jas.2016.1348 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Brazil is one of the world?s largest beef exporters, although the product has a low price due to quality issues. The meat exported by Brazil is considered medium and low quality by international buyers, mainly due to lack of tenderness. The predominant Zebu breeds (80% Nellore) are known for producing tougher beef than taurine breeds. Nonetheless, some studies have shown that there is substantial genetic variability for tenderness within the Nellore breed, although it is a difficult trait to improve by conventional selection methods. Therefore, the aim of this study was to perform a genomewide association study (GWAS) and a gene set enrichment analysis to identify genomic regions and biologically relevant pathways associated with meat tenderness in Polled Nellore cattle. Data consisted of Warner?Bratzler shear force values of LM from 427 Polled Nellore animals divided into 3 experimental slaughters (years 2005, 2008, and 2010). The animals were genotyped with either the Illumina BovineHD BeadChip (777k, on 61 samples) or the GGP Indicus HD chip (77k, on 366 samples). Single nucleotide polymorphisms were excluded when the call rate was <90%, the Hardy?Weinberg proportions P-value was <1% (Fisher exact test, Bonferroni adjusted), and the minor allele frequency was <1%. Imputation from the GGP Indicus HD chip to the Illumina BovineHD BeadChip was performed using the FImput program. Genomewide association analysis was performed using the Efficient Mixed Model Association eXpedited (EMMAx) and the population parameters previously determined (P3D) methods. The GWAS was complemented with a gene set enrichment analysis performed using the FatiGO procedure. Significant markers (P < 0.0001) explaining a larger proportion of variation than other significant SNPs were located on chromosomes 3, 13, 17, 20, 21, and 25, indicating QTL associated with meat tenderness throughout the genome. Additionally, gene set analysis identified 22 Gene Ontology functional terms and 2 InterPro entries that showed significant enrichment of genes associated with tenderness. The functional categories included protein tyrosine and serine/threonine kinase activity, calcium ion binding, lipid metabolic process, and growth factors, among others. These results help to elucidate the genetic architecture and metabolic pathways underlying this trait, which is of extreme economic and social importance to Brazil, because Nellore is the dominant beef cattle breed in the country. MenosAbstract: Brazil is one of the world?s largest beef exporters, although the product has a low price due to quality issues. The meat exported by Brazil is considered medium and low quality by international buyers, mainly due to lack of tenderness. The predominant Zebu breeds (80% Nellore) are known for producing tougher beef than taurine breeds. Nonetheless, some studies have shown that there is substantial genetic variability for tenderness within the Nellore breed, although it is a difficult trait to improve by conventional selection methods. Therefore, the aim of this study was to perform a genomewide association study (GWAS) and a gene set enrichment analysis to identify genomic regions and biologically relevant pathways associated with meat tenderness in Polled Nellore cattle. Data consisted of Warner?Bratzler shear force values of LM from 427 Polled Nellore animals divided into 3 experimental slaughters (years 2005, 2008, and 2010). The animals were genotyped with either the Illumina BovineHD BeadChip (777k, on 61 samples) or the GGP Indicus HD chip (77k, on 366 samples). Single nucleotide polymorphisms were excluded when the call rate was <90%, the Hardy?Weinberg proportions P-value was <1% (Fisher exact test, Bonferroni adjusted), and the minor allele frequency was <1%. Imputation from the GGP Indicus HD chip to the Illumina BovineHD BeadChip was performed using the FImput program. Genomewide association analysis was performed using the Efficient Mixed Model Associati... Mostrar Tudo |
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Palavras-Chave: |
Estudo de associação de genoma (GWAS); Gene ontology; Metabolic pathways; Parâmetro metabólico; Ternura da Carne. |
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Thesagro: |
Bos Indicus; Carne; Gado Nelore; Parâmetro Genético. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03425naa a2200301 a 4500
001 2074456
005 2017-08-25
008 2017 bl uuuu u00u1 u #d
024 7 $a10.2527/jas.2016.1348$2DOI
100 1 $aCASTRO, L. M. de
245 $aGenomewide association mapping and pathway analysis of meat tenderness in Polled Nellore cattle.$h[electronic resource]
260 $c2017
520 $aAbstract: Brazil is one of the world?s largest beef exporters, although the product has a low price due to quality issues. The meat exported by Brazil is considered medium and low quality by international buyers, mainly due to lack of tenderness. The predominant Zebu breeds (80% Nellore) are known for producing tougher beef than taurine breeds. Nonetheless, some studies have shown that there is substantial genetic variability for tenderness within the Nellore breed, although it is a difficult trait to improve by conventional selection methods. Therefore, the aim of this study was to perform a genomewide association study (GWAS) and a gene set enrichment analysis to identify genomic regions and biologically relevant pathways associated with meat tenderness in Polled Nellore cattle. Data consisted of Warner?Bratzler shear force values of LM from 427 Polled Nellore animals divided into 3 experimental slaughters (years 2005, 2008, and 2010). The animals were genotyped with either the Illumina BovineHD BeadChip (777k, on 61 samples) or the GGP Indicus HD chip (77k, on 366 samples). Single nucleotide polymorphisms were excluded when the call rate was <90%, the Hardy?Weinberg proportions P-value was <1% (Fisher exact test, Bonferroni adjusted), and the minor allele frequency was <1%. Imputation from the GGP Indicus HD chip to the Illumina BovineHD BeadChip was performed using the FImput program. Genomewide association analysis was performed using the Efficient Mixed Model Association eXpedited (EMMAx) and the population parameters previously determined (P3D) methods. The GWAS was complemented with a gene set enrichment analysis performed using the FatiGO procedure. Significant markers (P < 0.0001) explaining a larger proportion of variation than other significant SNPs were located on chromosomes 3, 13, 17, 20, 21, and 25, indicating QTL associated with meat tenderness throughout the genome. Additionally, gene set analysis identified 22 Gene Ontology functional terms and 2 InterPro entries that showed significant enrichment of genes associated with tenderness. The functional categories included protein tyrosine and serine/threonine kinase activity, calcium ion binding, lipid metabolic process, and growth factors, among others. These results help to elucidate the genetic architecture and metabolic pathways underlying this trait, which is of extreme economic and social importance to Brazil, because Nellore is the dominant beef cattle breed in the country.
650 $aBos Indicus
650 $aCarne
650 $aGado Nelore
650 $aParâmetro Genético
653 $aEstudo de associação de genoma (GWAS)
653 $aGene ontology
653 $aMetabolic pathways
653 $aParâmetro metabólico
653 $aTernura da Carne
700 1 $aROSA, G. J. de M.
700 1 $aLOPES, F. B.
700 1 $aREGITANO, L. C. de A.
700 1 $aROSA, A. J. de M.
700 1 $aMAGNABOSCO, C. de U.
773 $tJournal of Animal Science$gv. 95, n. 5, p. 1945-1956, 2017.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Classificação |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
11/01/2016 |
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Data da última atualização: |
01/03/2016 |
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Autoria: |
RIBEIRO, E. P.; MAGALHÃES, J. L.; RODRIGUES, A. A.; RODRIGUES, D. A.; FRAZÃO, M. A.; RODRIGUES, C. L. |
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Título: |
Análise inicial do super clone de eucalipto adubado com lodo de efluente suíno. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Florestal Brasileira, Colombo, v. 35, n. 84, p. 399-407, out./dez. 2015. |
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DOI: |
doi: 10.4336/2015.pfb.35.84.913 |
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Idioma: |
Português |
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Conteúdo: |
A geração de dejetos suínos aumenta gradativamente, resultando em preocupação com o destino final desse produto. Este trabalho teve como objetivo a análise do comportamento inicial do super clone de eucalipto (AEC 1528), adubado com lodo suíno em comparação à adubação química. O experimento foi realizado em delineamento inteiramente casualizado, com 5 tratamentos e 3 repetições, sendo estes com volumes: 0; 1,25; 2,5; 5 L de biossólido e adubação química. A irrigação foi com água proveniente de poço da Universidade de Rio Verde, aumentando a quantidade de acordo com o crescimento da planta. Aos 120 dias, avaliou-se a altura da planta, diâmetro do caule, relação diâmetro do caule/altura da planta, peso da matéria verde, peso da matéria seca, peso da raiz, área foliar e mortalidade. O lodo de efluentes suínos acarretou aumento significativo em relação à altura da planta, peso da matéria verde e peso da matéria seca. Para as demais características, o uso do lodo foi semelhante à adubação química para o híbrido de eucalipto nos diferentes volumes utilizados. Observou-se, que a aplicação de lodo da suínocultura é uma alternativa eficaz para o uso como adubo em plantios de eucalipto. |
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Palavras-Chave: |
Adubação orgânica; Biossólido; Hybrid; Organic manure. |
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Thesagro: |
Eucalipto; Hibrido. |
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Thesaurus NAL: |
biosolids; Eucalyptus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140345/1/913-12517-2-PB.pdf
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Marc: |
LEADER 02067naa a2200289 a 4500
001 2033551
005 2016-03-01
008 2015 bl uuuu u00u1 u #d
024 7 $adoi: 10.4336/2015.pfb.35.84.913$2DOI
100 1 $aRIBEIRO, E. P.
245 $aAnálise inicial do super clone de eucalipto adubado com lodo de efluente suíno.
260 $c2015
520 $aA geração de dejetos suínos aumenta gradativamente, resultando em preocupação com o destino final desse produto. Este trabalho teve como objetivo a análise do comportamento inicial do super clone de eucalipto (AEC 1528), adubado com lodo suíno em comparação à adubação química. O experimento foi realizado em delineamento inteiramente casualizado, com 5 tratamentos e 3 repetições, sendo estes com volumes: 0; 1,25; 2,5; 5 L de biossólido e adubação química. A irrigação foi com água proveniente de poço da Universidade de Rio Verde, aumentando a quantidade de acordo com o crescimento da planta. Aos 120 dias, avaliou-se a altura da planta, diâmetro do caule, relação diâmetro do caule/altura da planta, peso da matéria verde, peso da matéria seca, peso da raiz, área foliar e mortalidade. O lodo de efluentes suínos acarretou aumento significativo em relação à altura da planta, peso da matéria verde e peso da matéria seca. Para as demais características, o uso do lodo foi semelhante à adubação química para o híbrido de eucalipto nos diferentes volumes utilizados. Observou-se, que a aplicação de lodo da suínocultura é uma alternativa eficaz para o uso como adubo em plantios de eucalipto.
650 $abiosolids
650 $aEucalyptus
650 $aEucalipto
650 $aHibrido
653 $aAdubação orgânica
653 $aBiossólido
653 $aHybrid
653 $aOrganic manure
700 1 $aMAGALHÃES, J. L.
700 1 $aRODRIGUES, A. A.
700 1 $aRODRIGUES, D. A.
700 1 $aFRAZÃO, M. A.
700 1 $aRODRIGUES, C. L.
773 $tPesquisa Florestal Brasileira, Colombo$gv. 35, n. 84, p. 399-407, out./dez. 2015.
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Registro original: |
Embrapa Florestas (CNPF) |
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