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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
07/05/2013 |
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Data da última atualização: |
17/01/2024 |
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Autoria: |
LIMA-VERDE, I. B.; ROSSETTO, R.; MATOS, M. H. T.; CELESTINO, J. J. H.; BUENO, J. B.; SILVA, C. M. G.; FAUSTINO, L. R.; MORORÓ, M. B. S.; ARAÚJO, V. T.; CAMPELLO, C. C.; FIGUEIREDO, J. R. |
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Título: |
Androstenedione and follicle stimulating hormone involvement in the viability and development of goat preantral follicles in vitro. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Animal Reproduction, Belo Horizonte, v. 7, n. 2, p. 80-89, Apr./Jun. 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of androstenedione + FSH had viable preantral follicles similar to those observed in MEM+alone. In conclusion, androstenedione at 50 or 100 ng/ml, either associated with FSH or at 50 ng/ml alone, plays an important role in the maintenance of caprine preantral follicle viability and activation after only a short in vitro culture period. In addition, after 7 days MEM+alone was efficient in the maintenance of viability and in follicular activation, showing the importance of basic medium composition. MenosAbstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of andr... Mostrar Tudo |
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Palavras-Chave: |
Esteroidogenese; Foliculo pre-antral; FSH. |
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Thesagro: |
Caprino; Cultura in vitro; Endocrinologia; Hormônio; Ovário; Reprodução animal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03032naa a2200349 a 4500
001 1957239
005 2024-01-17
008 2010 bl uuuu u00u1 u #d
100 1 $aLIMA-VERDE, I. B.
245 $aAndrostenedione and follicle stimulating hormone involvement in the viability and development of goat preantral follicles in vitro.$h[electronic resource]
260 $c2010
520 $aAbstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of androstenedione + FSH had viable preantral follicles similar to those observed in MEM+alone. In conclusion, androstenedione at 50 or 100 ng/ml, either associated with FSH or at 50 ng/ml alone, plays an important role in the maintenance of caprine preantral follicle viability and activation after only a short in vitro culture period. In addition, after 7 days MEM+alone was efficient in the maintenance of viability and in follicular activation, showing the importance of basic medium composition.
650 $aCaprino
650 $aCultura in vitro
650 $aEndocrinologia
650 $aHormônio
650 $aOvário
650 $aReprodução animal
653 $aEsteroidogenese
653 $aFoliculo pre-antral
653 $aFSH
700 1 $aROSSETTO, R.
700 1 $aMATOS, M. H. T.
700 1 $aCELESTINO, J. J. H.
700 1 $aBUENO, J. B.
700 1 $aSILVA, C. M. G.
700 1 $aFAUSTINO, L. R.
700 1 $aMORORÓ, M. B. S.
700 1 $aARAÚJO, V. T.
700 1 $aCAMPELLO, C. C.
700 1 $aFIGUEIREDO, J. R.
773 $tAnimal Reproduction, Belo Horizonte$gv. 7, n. 2, p. 80-89, Apr./Jun. 2010.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
05/10/2011 |
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Data da última atualização: |
02/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SILVA, K. E. da; MARTINS, S. V.; RIBEIRO, C. A. A. S.; SANTOS, N. T.; AZEVEDO, C. P. de; MATOS, F. D. de A.; AMARAL, I. L. do. |
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Afiliação: |
KATIA EMIDIO DA SILVA, CPAA; Sebastião Venancio Martins, UFV; Carlos Antonio Alvares Soares Ribeiro, UFV; Nerilson Terra Santos, UFV; CELSO PAULO DE AZEVEDO, CPAA; Francisca Dionizia de Almeida Matos, INPA; Ieda Leão do Amaral, INPA. |
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Título: |
Floristic composition and similarity of 15 hectares in Central Amazon, Brazil. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Revista de Biologia Tropical, v. 59, n. 4, p. 1927-1938, Dec. 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
The Amazon region is one of the most diverse areas in the world. Research on high tropical forest diversity brings up relevant contributions to understand the mechanisms that result and support such diversity. In the present study we describe the species composition and diversity of 15 one-ha plots in the Amazonian terra firme dense forest in Brazil, and compare the floristic similarity of these plots with other nine one-ha plots. |
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Palavras-Chave: |
Terra firme. |
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Thesagro: |
Ecologia Florestal. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42879/1/Katia-Tropical.pdf
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Marc: |
LEADER 01068naa a2200217 a 4500
001 1902390
005 2018-02-02
008 2011 bl uuuu u00u1 u #d
100 1 $aSILVA, K. E. da
245 $aFloristic composition and similarity of 15 hectares in Central Amazon, Brazil.
260 $c2011
520 $aThe Amazon region is one of the most diverse areas in the world. Research on high tropical forest diversity brings up relevant contributions to understand the mechanisms that result and support such diversity. In the present study we describe the species composition and diversity of 15 one-ha plots in the Amazonian terra firme dense forest in Brazil, and compare the floristic similarity of these plots with other nine one-ha plots.
650 $aEcologia Florestal
653 $aTerra firme
700 1 $aMARTINS, S. V.
700 1 $aRIBEIRO, C. A. A. S.
700 1 $aSANTOS, N. T.
700 1 $aAZEVEDO, C. P. de
700 1 $aMATOS, F. D. de A.
700 1 $aAMARAL, I. L. do
773 $tRevista de Biologia Tropical$gv. 59, n. 4, p. 1927-1938, Dec. 2011.
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