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Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
16/04/2019 |
Data da última atualização: |
20/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GÓMEZ, M.; PARANHOS, B. A. J.; SILVA, J. G.; LIMA, M. A. C. de; SILVA, M. A.; MACEDO, A. T.; VIRGINIO, J. F.; WALDER, J. M. M. |
Afiliação: |
MAYLEN GÓMEZ; BEATRIZ AGUIAR GIORDANO PARANHOS, CPATSA; JANISETE G. SILVA; MARIA AUXILIADORA COELHO DE LIMA, CPATSA; MÁRCIO A. SILVA; ALINE T. MACEDO; JAIR F. VIRGINIO; JULIO M. M. WALDER. |
Título: |
Oviposition preference of Ceratitis capitata (Diptera: Tephritidae) at different times after pruning Italia table grapes grown in Brazil. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Journal of Insect Science, v. 19, n. 1, p. 1-7, 2019. |
DOI: |
10.1093/jisesa/iey136 |
Idioma: |
Inglês |
Conteúdo: |
Studies investigating oviposition preference of Ceratitis capitata (Wiedemann, 1824) in table grapes (Vitis vinifera L.) cultivated in the São Francisco River Valley can provide important information to guide control measures, therefore minimizing damage caused by this species in the region. The aim of this study was to evaluate the oviposition preference of C. capitata females for berries cv. ?Italia? collected at five different times (60, 70, 80, 90, and 100 d after production pruning [DAPP]), representing five grapevine growth stages. We also assessed the performance of immature stages of C. capitata regarding the physiological development of the berry. The total soluble solid content was significantly higher in berries at more advanced physiological developmental stages (90 and 100 DAPP). Conversely, these berries showed the lowest values of firmness and titratable acidity. With the onset of physiological development, the average number of punctures per berry increased and reached 5.2 per berry in 100 DAPP berries. The infestation level and pupal weight showed a positive correlation with the growth stage. The highest recovery of pupae was observed in 100 DAPP berries (1.54). Pupal viability values ranged from 50.9 to 64.7% independent of berry maturity stage. The physiological developmental stage of the berry did not affect offspring sex ratio. Results obtained suggest that regardless of the female preference for laying eggs on berries in a more advanced physiological developmental stage, females can initiate the attack to bunches of this cultivar at 60 DAPP, even if the berries have unfavorable physicochemical parameters for oviposition and development of larvae. MenosStudies investigating oviposition preference of Ceratitis capitata (Wiedemann, 1824) in table grapes (Vitis vinifera L.) cultivated in the São Francisco River Valley can provide important information to guide control measures, therefore minimizing damage caused by this species in the region. The aim of this study was to evaluate the oviposition preference of C. capitata females for berries cv. ?Italia? collected at five different times (60, 70, 80, 90, and 100 d after production pruning [DAPP]), representing five grapevine growth stages. We also assessed the performance of immature stages of C. capitata regarding the physiological development of the berry. The total soluble solid content was significantly higher in berries at more advanced physiological developmental stages (90 and 100 DAPP). Conversely, these berries showed the lowest values of firmness and titratable acidity. With the onset of physiological development, the average number of punctures per berry increased and reached 5.2 per berry in 100 DAPP berries. The infestation level and pupal weight showed a positive correlation with the growth stage. The highest recovery of pupae was observed in 100 DAPP berries (1.54). Pupal viability values ranged from 50.9 to 64.7% independent of berry maturity stage. The physiological developmental stage of the berry did not affect offspring sex ratio. Results obtained suggest that regardless of the female preference for laying eggs on berries in a more advanced physiological de... Mostrar Tudo |
Palavras-Chave: |
Mediterranean fruit fly; Uva de mesa; Vale do São Francisco. |
Thesagro: |
Ceratitis Capitata; Controle Biológico; Inseto Para Controle Biológico; Maturação; Mosca das Frutas; Uva; Vitis Vinifera. |
Thesaurus Nal: |
Ceratitis capitata Yoyo virus; Vitis. |
Categoria do assunto: |
O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/195896/1/Dora-2019.pdf
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Marc: |
LEADER 02802naa a2200361 a 4500 001 2108261 005 2019-11-20 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1093/jisesa/iey136$2DOI 100 1 $aGÓMEZ, M. 245 $aOviposition preference of Ceratitis capitata (Diptera$bTephritidae) at different times after pruning Italia table grapes grown in Brazil.$h[electronic resource] 260 $c2019 520 $aStudies investigating oviposition preference of Ceratitis capitata (Wiedemann, 1824) in table grapes (Vitis vinifera L.) cultivated in the São Francisco River Valley can provide important information to guide control measures, therefore minimizing damage caused by this species in the region. The aim of this study was to evaluate the oviposition preference of C. capitata females for berries cv. ?Italia? collected at five different times (60, 70, 80, 90, and 100 d after production pruning [DAPP]), representing five grapevine growth stages. We also assessed the performance of immature stages of C. capitata regarding the physiological development of the berry. The total soluble solid content was significantly higher in berries at more advanced physiological developmental stages (90 and 100 DAPP). Conversely, these berries showed the lowest values of firmness and titratable acidity. With the onset of physiological development, the average number of punctures per berry increased and reached 5.2 per berry in 100 DAPP berries. The infestation level and pupal weight showed a positive correlation with the growth stage. The highest recovery of pupae was observed in 100 DAPP berries (1.54). Pupal viability values ranged from 50.9 to 64.7% independent of berry maturity stage. The physiological developmental stage of the berry did not affect offspring sex ratio. Results obtained suggest that regardless of the female preference for laying eggs on berries in a more advanced physiological developmental stage, females can initiate the attack to bunches of this cultivar at 60 DAPP, even if the berries have unfavorable physicochemical parameters for oviposition and development of larvae. 650 $aCeratitis capitata Yoyo virus 650 $aVitis 650 $aCeratitis Capitata 650 $aControle Biológico 650 $aInseto Para Controle Biológico 650 $aMaturação 650 $aMosca das Frutas 650 $aUva 650 $aVitis Vinifera 653 $aMediterranean fruit fly 653 $aUva de mesa 653 $aVale do São Francisco 700 1 $aPARANHOS, B. A. J. 700 1 $aSILVA, J. G. 700 1 $aLIMA, M. A. C. de 700 1 $aSILVA, M. A. 700 1 $aMACEDO, A. T. 700 1 $aVIRGINIO, J. F. 700 1 $aWALDER, J. M. M. 773 $tJournal of Insect Science$gv. 19, n. 1, p. 1-7, 2019.
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Embrapa Semiárido (CPATSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
19/10/2021 |
Data da última atualização: |
09/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SOARES, J. C.; SOARES, A. C.; RODRIGUES, V. C.; OITICICA, P. R. A.; RAYMUNDO-PEREIRA, P. A.; BOTT-NETO, J. L.; BUSCAGLIA, L. A.; CASTRO, L. D. C. de; RIBAS, L. C.; SCABINI, L.; BRAZACA, L. C.; CORREA, D. S.; MATTOSO, L. H. C.; OLIVEIRA, M. C. F. de; CARVALHO, A. C. P. L. de; CARRILHO, E.; BRUNO, O. M.; MELENDEZ, M. E.; OLIVEIRA JR, O. N. |
Afiliação: |
DANIEL SOUZA CORREA, CNPDIA; LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
Título: |
Detection of a SARS-CoV-2 sequence with genosensors using data analysis based on information visualization and machine learning techniques. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Materials Chemistry Frontiers, v. 5, 2021. |
Páginas: |
5658-5670 |
DOI: |
10.1039/d1qm00665g |
Idioma: |
Português |
Conteúdo: |
We report on genosensors to detect an ssDNA sequence from the SARS-CoV-2 genome, which mimics the GU280 gp10 gene (coding the viral nucleocapsid phosphoprotein), using four distinct principles of detection and treating the data with information visualization and machine learning techniques. Genosensors were fabricated on either gold (Au) interdigitated electrodes for electrical and electrochemical measurements or on Au nanoparticles on a glass slide for optical measurements. They contained a matrix of 11-mercaptoundecanoic acid (11-MUA) self-assembled monolayer (SAM) onto which a layer of capture probe (cpDNA) sequence was immobilized. Detection was performed using electrical and electrochemical impedance spectroscopies and localized surface plasmon resonance (LSPR). The highest sensitivity was reached with impedance spectroscopy, including using a low-cost (US$ 100) homemade impedance analyzer. Complementary ssDNA sequences were detected with a detection limit of 0.5 aM (0.3 copy per mL). This performance may be attributed to the high sensitivity of the electrical impedance technique combined with an appropriate arrangement of the sequences on the electrodes and hybridization between the complementary sequences, as inferred from polarizationmodulated infrared reflection absorption spectroscopy (PM-IRRAS). The selectivity of the genosensor was confirmed by plotting the impedance spectroscopy data with a multidimensional projection technique (interactive document mapping, IDMAP), where a clear separation was observed among the samples of the complementary DNA sequence at various concentrations and from buffer samples containing a non-complementary sequence and other DNA biomarkers. The diagnosis of SARS-CoV-2 mimicking sequences was also achieved with machine learning techniques applied to scanning electron microscope images taken from genosensors exposed to distinct concentrations of the complementary ssDNA sequences. In summary, the genosensors proposed here are promising for detecting SARS-CoV2 genetic material (RNA) in biological fluids in point-of-care settings MenosWe report on genosensors to detect an ssDNA sequence from the SARS-CoV-2 genome, which mimics the GU280 gp10 gene (coding the viral nucleocapsid phosphoprotein), using four distinct principles of detection and treating the data with information visualization and machine learning techniques. Genosensors were fabricated on either gold (Au) interdigitated electrodes for electrical and electrochemical measurements or on Au nanoparticles on a glass slide for optical measurements. They contained a matrix of 11-mercaptoundecanoic acid (11-MUA) self-assembled monolayer (SAM) onto which a layer of capture probe (cpDNA) sequence was immobilized. Detection was performed using electrical and electrochemical impedance spectroscopies and localized surface plasmon resonance (LSPR). The highest sensitivity was reached with impedance spectroscopy, including using a low-cost (US$ 100) homemade impedance analyzer. Complementary ssDNA sequences were detected with a detection limit of 0.5 aM (0.3 copy per mL). This performance may be attributed to the high sensitivity of the electrical impedance technique combined with an appropriate arrangement of the sequences on the electrodes and hybridization between the complementary sequences, as inferred from polarizationmodulated infrared reflection absorption spectroscopy (PM-IRRAS). The selectivity of the genosensor was confirmed by plotting the impedance spectroscopy data with a multidimensional projection technique (interactive document mapping, IDM... Mostrar Tudo |
Palavras-Chave: |
Genetic material (RNA); SsDNA; Viral nucleocapsid phosphoprotein). |
Categoria do assunto: |
-- |
Marc: |
LEADER 03303naa a2200397 a 4500 001 2135418 005 2022-06-09 008 2021 bl uuuu u00u1 u #d 024 7 $a10.1039/d1qm00665g$2DOI 100 1 $aSOARES, J. C. 245 $aDetection of a SARS-CoV-2 sequence with genosensors using data analysis based on information visualization and machine learning techniques.$h[electronic resource] 260 $c2021 300 $a5658-5670 520 $aWe report on genosensors to detect an ssDNA sequence from the SARS-CoV-2 genome, which mimics the GU280 gp10 gene (coding the viral nucleocapsid phosphoprotein), using four distinct principles of detection and treating the data with information visualization and machine learning techniques. Genosensors were fabricated on either gold (Au) interdigitated electrodes for electrical and electrochemical measurements or on Au nanoparticles on a glass slide for optical measurements. They contained a matrix of 11-mercaptoundecanoic acid (11-MUA) self-assembled monolayer (SAM) onto which a layer of capture probe (cpDNA) sequence was immobilized. Detection was performed using electrical and electrochemical impedance spectroscopies and localized surface plasmon resonance (LSPR). The highest sensitivity was reached with impedance spectroscopy, including using a low-cost (US$ 100) homemade impedance analyzer. Complementary ssDNA sequences were detected with a detection limit of 0.5 aM (0.3 copy per mL). This performance may be attributed to the high sensitivity of the electrical impedance technique combined with an appropriate arrangement of the sequences on the electrodes and hybridization between the complementary sequences, as inferred from polarizationmodulated infrared reflection absorption spectroscopy (PM-IRRAS). The selectivity of the genosensor was confirmed by plotting the impedance spectroscopy data with a multidimensional projection technique (interactive document mapping, IDMAP), where a clear separation was observed among the samples of the complementary DNA sequence at various concentrations and from buffer samples containing a non-complementary sequence and other DNA biomarkers. The diagnosis of SARS-CoV-2 mimicking sequences was also achieved with machine learning techniques applied to scanning electron microscope images taken from genosensors exposed to distinct concentrations of the complementary ssDNA sequences. In summary, the genosensors proposed here are promising for detecting SARS-CoV2 genetic material (RNA) in biological fluids in point-of-care settings 653 $aGenetic material (RNA) 653 $aSsDNA 653 $aViral nucleocapsid phosphoprotein) 700 1 $aSOARES, A. C. 700 1 $aRODRIGUES, V. C. 700 1 $aOITICICA, P. R. A. 700 1 $aRAYMUNDO-PEREIRA, P. A. 700 1 $aBOTT-NETO, J. L. 700 1 $aBUSCAGLIA, L. A. 700 1 $aCASTRO, L. D. C. de 700 1 $aRIBAS, L. C. 700 1 $aSCABINI, L. 700 1 $aBRAZACA, L. C. 700 1 $aCORREA, D. S. 700 1 $aMATTOSO, L. H. C. 700 1 $aOLIVEIRA, M. C. F. de 700 1 $aCARVALHO, A. C. P. L. de 700 1 $aCARRILHO, E. 700 1 $aBRUNO, O. M. 700 1 $aMELENDEZ, M. E. 700 1 $aOLIVEIRA JR, O. N. 773 $tMaterials Chemistry Frontiers$gv. 5, 2021.
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