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| Registros recuperados : 92 | |
| 42. |  | POZZER, L.; NAGATA, T.; LIMA, M. I.; KITAJIMA, E. W.; RESENDE, R. de O.; AVILA, A. C. de. A new tospovirus naturally infecting cucurbitaceae in Brazil. Fitopatologia Brasileira, Brasilia, v.19, p.321, ago. 1994. Resumo. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| 44. | | TORRES, A. C.; FAJARDO, T. V. M.; DUSI, A. N.; RESENDE, R. de O.; BUSO, J. A. Shoot tip culture and thermotherapy of recovering virus-free plants of garlic. Horticultura Brasileira, Brasilia, v.18, n.3, p.192-195, nov. 2000.| Biblioteca(s): Embrapa Hortaliças. |
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| 47. | | OLIVEIRA, E. de; RESENDE, R. de O.; PECCI, M. de la P. G.; LAGUNA, I. G.; HERRERA, P.; CRUZ, I. Incidência de viroses e enfezamentos e estimativas de perdas causadas por molicutes em milho no Paraná. Pesquisa Agropecuária Brasileira, Brasília, v. 38, n. 1, p. 19-25, jan. 2003.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 48. | | REZENDE, J. A. M.; GALLETI, S. R.; POZZER, L.; RESENDE, R. de O.; AVILA, A. C. de. Incidence, biological and serological characteristics of a tospovirus in experimental fields of zucchini in Sao Paulo State, Brazil. Fitopatologia Brasileira, Brasilia, v.22, n.1, p.92-98, mar. 1997.| Biblioteca(s): Embrapa Hortaliças. |
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| 49. | | AVILA, A. C. de; HAAN, P. de; KITAJIMA, E. W.; RESENDE, R. de O.; GOLDBACH, R. W.; PETERS, D. Characterization of a distinct isolate of tomato spotted wilt virus (TSWV) from impatiens in the Netherlands. Fitopatologia Brasileira, Brasilia, v.16, n.2, p.25, jun. 1991. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 50. | | POZZER, L.; BEZERRA, I. C.; KORMELINK, R.; PRINS, M.; PETERS, D.; RESENDE, R. de O.; AVILA, A. C. de. Characterization of a tospovirus isolate of iris yellow spot virus associated with a disease in onion fields in Brazil. Plant Diseases, v.83, n.4, p.345-350, Apr. 1999.| Biblioteca(s): Embrapa Hortaliças. |
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| 51. | | AVILA, A. C. de; RESENDE, R. de O.; BEZERRA, I. C.; POZZER, L.; NAGATA, T.; KITAJIMA, E. W. The potential use of Polymerase Chain Reaction on the detection of tospovirus species. Fitopatologia Brasileira, Brasilia, v.20, p.301, ago. 1995. Suplemento. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 52. | | NAGATA, T.; RESENDE, R. de O.; KITAJIMA, E. W.; COSTA, H.; INOUE-NAGATA, A.K.; AVILA, A. C. de. First report of natural occurrence of Zucchini Lethal Chlorosis Tospovirus on cucumber and Chrysanthemum Stem Necrosis Tospovirus on tomato in Brazil. Plant Disease, v.82, n.12, p.1403, Dec. 1998.| Biblioteca(s): Embrapa Hortaliças. |
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| 53. | | RESENDE, R. de O.; HAAN, P. de; AVILA, A. C. de; KITAJIMA, E. W.; KORMELINK, R.; GOLDBACH, R.; PETERS, D. Generation of envelope and defective interfering RNA mutants of tomato spotted wilt virus by machanical passage. Journal of General Virology, v. 72, p.2335-2383, 1991.| Biblioteca(s): Embrapa Hortaliças. |
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| 54. | | SILVA, M. S.; MARTINS, C. R. F.; BEZERRA, M. I.; NAGATA, T.; ÁVILA, A. C.; RESENDE, R. de O. Analysis of conserved domais present in the movemet protein of tospoviruses. Virus Reviews & Research, Florianópolis, v. 5, n. 2, p. 193, 2000. Suplemento 1. Resumo. Trabalho apresentado no 11º National Meeting of Virology, São Lourenço, 2000.| Biblioteca(s): Embrapa Hortaliças. |
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| 55. | | POZZER, L.; NAGATA, T.; LIMA, M. I.; KITAJIMA, E. W.; RESENDE, R. de O.; AVILA, A. C. de. "Sapeca" an onion disease in the sub-medio Sao Francisco region, Brazil, is caused by a tospovirus with a serologically distinct nucleocapsid protein. Fitopatologia Brasileira, Brasilia, v.19, p.321, ago. 1994. Resumo. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| 56. | | POZZER, L.; RESENDE, R. de O.; BEZERRA, M. I.; NAGATA, T.; LIMA, M. I.; KITAJIMA, E. W.; AVILA, A. C. Zucchini lethal chlorotic virus(ZCLV): a proposed new species in the tospovirus genus. Fitopatologia Brasileira, Brasilia, v.21, p.432, ago. 1996. Resumo. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| 57. | | BEZERRA, B. M.; PINHEIRO-LIMA, B.; CALAÇA, M. M.; SOUZA, C. do A.; RESENDE, R. de O.; BOITEUX, L. S.; PEREIRA-CARVALHO, R. de C. Busca de genótipos de berinjela resistentes à Potato vírus Y e Groundnut ringspot vírus. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 p. Resumo 303_1| Biblioteca(s): Embrapa Hortaliças. |
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| 59. | | BEZERRA, M. I.; POZZER, L.; NAGATA, R. de O.; LIMA, M. I.; KITAJIMA, E. W.; AVILA, A. C.; RESENDE, R. de O. Chrysanthemum stem necrosis (CSNV): a proposed new species in the tospovirus genus. Fitopatologia Brasileira, Brasilia, v.21, p.430, ago. 1996. Resumo. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| 60. | | ALMEIDA, M. M. S. de; JAIN, S.; BARROSO, P. A. V.; HOFFMANN, L. V.; LUCENA, M. G. de; RESENDE, R. de O.; INOUE-NAGATA, A. K. Complete sequence of a New Bipartite begomovirus infecting cotton plants in Brazil. Genome Announcements, v. 1, n. 6, p. 1, Nov./Dec. 2013.| Biblioteca(s): Embrapa Algodão. |
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| Registros recuperados : 92 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Hortaliças. |
|
Data corrente: |
31/03/1993 |
|
Data da última atualização: |
05/03/2024 |
|
Autoria: |
RESENDE, R. de O.; HAAN, P. de; VOSSEN, E. van de; AVILA, A. C. de; GOLDBACH, R.; PETERS, D. |
|
Afiliação: |
RENATO DE OLIVEIRA RESENDE; PETER DE HAAN; EDWIN VAN DE VOSSEN; ANTONIO CARLOS DE AVILA, CNPH; ROB GOLDBACH; DICK PETERS. |
|
Título: |
Defective interfering L RNA segments of tomato spotted wilt virus retain both virus genome termini and have extensive internal deletions. |
|
Ano de publicação: |
1992 |
|
Fonte/Imprenta: |
Journal of General Virology, v. 73, p. 2509-2516, 1992. |
|
DOI: |
https://doi.org/10.1099/0022-1317-73-10-2509 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Defective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another containing the repeated sequences, during the replication of the viral complementary L RNA segment. MenosDefective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another conta... Mostrar Tudo |
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Palavras-Chave: |
Caracterizacao; Characterization; DI L RNA; Generation; Geracao; Maintenance; TSWV; Vira-Cabeca. |
|
Thesagro: |
Manutenção. |
|
Thesaurus NAL: |
Tomato spotted wilt virus. |
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Categoria do assunto: |
-- |
|
Marc: |
LEADER 02548naa a2200313 a 4500
001 1749565
005 2024-03-05
008 1992 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1099/0022-1317-73-10-2509$2DOI
100 1 $aRESENDE, R. de O.
245 $aDefective interfering L RNA segments of tomato spotted wilt virus retain both virus genome termini and have extensive internal deletions.$h[electronic resource]
260 $c1992
520 $aDefective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another containing the repeated sequences, during the replication of the viral complementary L RNA segment.
650 $aTomato spotted wilt virus
650 $aManutenção
653 $aCaracterizacao
653 $aCharacterization
653 $aDI L RNA
653 $aGeneration
653 $aGeracao
653 $aMaintenance
653 $aTSWV
653 $aVira-Cabeca
700 1 $aHAAN, P. de
700 1 $aVOSSEN, E. van de
700 1 $aAVILA, A. C. de
700 1 $aGOLDBACH, R.
700 1 $aPETERS, D.
773 $tJournal of General Virology$gv. 73, p. 2509-2516, 1992.
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