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| Registros recuperados : 92 | |
| 9. |  | SANTOS, C. D. G.; AVILA, A. C. de; BEZERRA, I. C.; RESENDE, R. de O. Interacao geminivirus de tomate e Bemisia argentifolli: hospedeiros, aquisicao do virus, transmissao e deteccao no vetor. In: ENCONTRO LATINO-AMERICANO E DO CARIBE SOBRE MOSCAS BRANCAS E GEMINIVIRUS, 8., 1999, Recife, PE. Anais [e] mini-resumos... Recife: IPA, 1999. p.123. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | FAJARDO, T. V. M.; AVILA, A. C. de; BUSO, J. A.; RESENDE, R. de O. Molecular characterization of garlic common latent virus(GCLV), a carlavirus, in garlic viral complex. Fitopatologia Brasileira, Brasilia, v.22, p.334, 1997. Suplemento. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 11. | | FAJARDO, T. V. M.; AVILA, A. C. de; BUSO, J. A.; RESENDE, R. de O. Molecular characterization of garlic viral complex. Virus Reviews Research, Sao Paulo, v.2, n,1-2, p.191, nov. 1997. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 16. | | CUNHA, L. C. V. da; RESENDE, R, de O.; NAGATA, T.; INOUE-NAGATA, A. K. Distinct features of pepper yellow mosaic vírus isolates from tomato and sweetpepper. Brasília, Fitopatologia Brasileira, v. 29, n. 6, p. 663-667, maio/jun. 2004.| Biblioteca(s): Embrapa Hortaliças. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Hortaliças. |
|
Data corrente: |
25/01/2005 |
|
Data da última atualização: |
25/01/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
Internacional - A |
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Autoria: |
MEDEIROS, R. B. de; FIGUEIREDO, J.; RESENDE, R. de O.; AVILA, A. C. de. |
|
Afiliação: |
RICARDO B. DE MEDEIROS, UNIVERSIDADE DE BRASÍLIA; JULIANA FIQUEIREDO, UNIVERSIDADE DE BRASÍLIA; RENATO DE O. RESENDE, UNIVERSIDADE DE BRASÍLIA; ANTONIO CARLOS DE AVILA, CNPH. |
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Título: |
Expression of a viral polymerase-bound host factor turns human cell lines permissive to a plant-and insect-infecting virus. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
PNAS, v. 102, n. 4, p. 1175-1180, jan. 2005. |
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Idioma: |
Inglês |
|
Conteúdo: |
Tospoviruses are the only plant-infecting members of theBunya-viridaefamily of ambisense ssRNA viruses. Tomato spotted wilttospovirus (TSWV), the type-member, also causes mild infection onits main insect vector,Frankliniella occidentalis. Herein, we iden-tified anF. occidentalisputative transcription factor (FoTF) thatbinds to the TSWV RNA-dependent RNA polymerase and to viralRNA. Usingin vitroRNA synthesis assays, we show that addition ofpurified FoTF improves viral replication, but not transcription.Expression of FoTF deletion mutants, unable to bind the RNA-dependent RNA polymerase or viral RNA, blocks TSWV replicationinF. occidentaliscells. Finally, expression of FoTF wild-type turnshuman cell lines permissive to TSWV replication. These data indi-cate that FoTF is a host factor required for TSWV replicationin vitroandin vivo, provide an experimental system that could be used tocompare molecular defense mechanisms in plant, insect, and hu-man cells against the same pathogen (TSWV), and could lead to abetter understanding of evolutionary processes of ambisense RNAviruses. |
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Palavras-Chave: |
Replicação; TSWV. |
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Thesagro: |
Vírus. |
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Thesaurus NAL: |
Frankliniella occidentalis. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01722naa a2200205 a 4500
001 1777616
005 2024-01-25
008 2005 bl uuuu u00u1 u #d
100 1 $aMEDEIROS, R. B. de
245 $aExpression of a viral polymerase-bound host factor turns human cell lines permissive to a plant-and insect-infecting virus.$h[electronic resource]
260 $c2005
520 $aTospoviruses are the only plant-infecting members of theBunya-viridaefamily of ambisense ssRNA viruses. Tomato spotted wilttospovirus (TSWV), the type-member, also causes mild infection onits main insect vector,Frankliniella occidentalis. Herein, we iden-tified anF. occidentalisputative transcription factor (FoTF) thatbinds to the TSWV RNA-dependent RNA polymerase and to viralRNA. Usingin vitroRNA synthesis assays, we show that addition ofpurified FoTF improves viral replication, but not transcription.Expression of FoTF deletion mutants, unable to bind the RNA-dependent RNA polymerase or viral RNA, blocks TSWV replicationinF. occidentaliscells. Finally, expression of FoTF wild-type turnshuman cell lines permissive to TSWV replication. These data indi-cate that FoTF is a host factor required for TSWV replicationin vitroandin vivo, provide an experimental system that could be used tocompare molecular defense mechanisms in plant, insect, and hu-man cells against the same pathogen (TSWV), and could lead to abetter understanding of evolutionary processes of ambisense RNAviruses.
650 $aFrankliniella occidentalis
650 $aVírus
653 $aReplicação
653 $aTSWV
700 1 $aFIGUEIREDO, J.
700 1 $aRESENDE, R. de O.
700 1 $aAVILA, A. C. de
773 $tPNAS$gv. 102, n. 4, p. 1175-1180, jan. 2005.
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