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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
11/09/2017 |
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Data da última atualização: |
06/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMPOS, C. G.; VERAS, H. C. T.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; ARAÚJO, K. P.; RODRIGUES, C. M.; ALMEIDA, J. R. M. de; ABDELNUR, P. V. |
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Afiliação: |
CHRISTIANE GONÇALVES CAMPOS, Universidade Federal de Goiás; HENRIQUE CÉSAR TEIXEIRA VERAS, Universidade de Brasília; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; PATRICIA PINTO KALIL G COSTA, CNPAE; KATIÚSCIA PEREIRA ARAÚJO; CLENILSON MARTINS RODRIGUES, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE. |
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Título: |
New protocol based on UHPLC-MS/MS for quantitation of metabolites in xylose-fermenting yeasts. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of the American Society for Mass Spectrometry, v. 28, n. 12, p. 2646-2657, 2017. |
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Páginas: |
p. 2646-2657 |
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DOI: |
10.1007/s13361-017-1786-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Xylose fermentation is a bottleneck in second-generation ethanol production. As such, a comprehensive understanding of xylose metabolism in naturally xylose-fermenting yeasts is essential for prospection and construction of recombinant yeast strains. The objective of the current study was to establish a reliable metabolomics protocol for quantification of key metabolites of xylose catabolism pathways in yeast, and to apply this protocol to Spathaspora arborariae. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites, and afterwards, sample preparation was optimized to examine yeast intracellular metabolites. S. arborariae was cultivated using xylose as a carbon source under aerobic and oxygen-limited conditions. Ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) were shown to efficiently quantify 14 and 5 metabolites, respectively, in a more rapid chromatographic protocol than previously described. Thirteen and eleven metabolites were quantified in S. arborariae under aerobic and oxygen-limited conditions, respectively. This targeted metabolomics protocol is shown here to quantify a total of 19 metabolites, including sugars, phosphates, coenzymes, monosaccharides, and alcohols, from xylose catabolism pathways (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle) in yeast. Furthermore, to our knowledge, this is the first time that intracellular metabolites have been quantified in S. arborariae after xylose consumption. The results indicated that fine control of oxygen levels during fermentation is necessary to optimize ethanol production by S. arborariae. The protocol presented here may be applied to other yeast species and could support yeast genetic engineering to improve second generation ethanol production. Graphical Abstract ?. MenosAbstract: Xylose fermentation is a bottleneck in second-generation ethanol production. As such, a comprehensive understanding of xylose metabolism in naturally xylose-fermenting yeasts is essential for prospection and construction of recombinant yeast strains. The objective of the current study was to establish a reliable metabolomics protocol for quantification of key metabolites of xylose catabolism pathways in yeast, and to apply this protocol to Spathaspora arborariae. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites, and afterwards, sample preparation was optimized to examine yeast intracellular metabolites. S. arborariae was cultivated using xylose as a carbon source under aerobic and oxygen-limited conditions. Ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) were shown to efficiently quantify 14 and 5 metabolites, respectively, in a more rapid chromatographic protocol than previously described. Thirteen and eleven metabolites were quantified in S. arborariae under aerobic and oxygen-limited conditions, respectively. This targeted metabolomics protocol is shown here to quantify a total of 19 metabolites, including sugars, phosphates, coenzymes, monosaccharides, and alcohols, from xylose catabolism pathways (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle) in yeast. Furthermore, to our knowledge, this is... Mostrar Tudo |
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Palavras-Chave: |
Spathaspora arborariae; UHPLC-MS/MS; Xylose fermentation. |
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Thesaurus Nal: |
Mass spectrometry; Metabolomics; Xylose. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163703/1/Abdelnur-ASMassSoectiometry-2017.pdf
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Marc: |
LEADER 02860naa a2200301 a 4500
001 2075378
005 2021-04-06
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1007/s13361-017-1786-9$2DOI
100 1 $aCAMPOS, C. G.
245 $aNew protocol based on UHPLC-MS/MS for quantitation of metabolites in xylose-fermenting yeasts.$h[electronic resource]
260 $c2017
300 $ap. 2646-2657
520 $aAbstract: Xylose fermentation is a bottleneck in second-generation ethanol production. As such, a comprehensive understanding of xylose metabolism in naturally xylose-fermenting yeasts is essential for prospection and construction of recombinant yeast strains. The objective of the current study was to establish a reliable metabolomics protocol for quantification of key metabolites of xylose catabolism pathways in yeast, and to apply this protocol to Spathaspora arborariae. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites, and afterwards, sample preparation was optimized to examine yeast intracellular metabolites. S. arborariae was cultivated using xylose as a carbon source under aerobic and oxygen-limited conditions. Ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) were shown to efficiently quantify 14 and 5 metabolites, respectively, in a more rapid chromatographic protocol than previously described. Thirteen and eleven metabolites were quantified in S. arborariae under aerobic and oxygen-limited conditions, respectively. This targeted metabolomics protocol is shown here to quantify a total of 19 metabolites, including sugars, phosphates, coenzymes, monosaccharides, and alcohols, from xylose catabolism pathways (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle) in yeast. Furthermore, to our knowledge, this is the first time that intracellular metabolites have been quantified in S. arborariae after xylose consumption. The results indicated that fine control of oxygen levels during fermentation is necessary to optimize ethanol production by S. arborariae. The protocol presented here may be applied to other yeast species and could support yeast genetic engineering to improve second generation ethanol production. Graphical Abstract ?.
650 $aMass spectrometry
650 $aMetabolomics
650 $aXylose
653 $aSpathaspora arborariae
653 $aUHPLC-MS/MS
653 $aXylose fermentation
700 1 $aVERAS, H. C. T.
700 1 $aRIBEIRO, J. A. de A.
700 1 $aCOSTA, P. P. K. G.
700 1 $aARAÚJO, K. P.
700 1 $aRODRIGUES, C. M.
700 1 $aALMEIDA, J. R. M. de
700 1 $aABDELNUR, P. V.
773 $tJournal of the American Society for Mass Spectrometry$gv. 28, n. 12, p. 2646-2657, 2017.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Biblioteca |
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Classificação |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
28/11/2019 |
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Data da última atualização: |
17/12/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
NOVOTNY, E. H.; TURETTA, A. P. D.; RESENDE, M. F. de; REBELLO, C. M. |
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Afiliação: |
ETELVINO HENRIQUE NOVOTNY, CNPS; ANA PAULA DIAS TURETTA, CNPS; MICHELE FABRI DE RESENDE, UFF; CATARINA MENDES REBELLO, UNIRIO. |
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Título: |
The quality of soil organic matter, accessed by 13C Solid State NMR, is more important than its content concerning pesticide adsorption. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: AUSTRALIAN AND NEW ZEALAND SOCIETY FOR MAGNETIC RESONANCE CONFERENCE, 12., 2019, Cape Naturaliste. Conference handbook. Randwick: Australian and New Zealand Society for Magnetic Resonance, 2019. p. 76. ANZMAG 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
The need for food will increase by 59-98% from 2005 to 2050, and its global growth production has been achieved mainly through the intensive use of inputs such as pesticides and chemical fertilizers. Once released to the soil, sorption (represented by Kd values) and degradation are two governing processes that determine the distribution and persistence of pesticides. In spite of the huge dataset, the only apparent generalization is the high correlation between Kd and soil organic matter (SOM) content. This is because the SOM is the main adsorption site for pesticides. Seeking to normalize the experimental data and to access the pesticides mobility, the KOC value is calculated: Kd normalized by soil organic C content (C). |
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Thesagro: |
Matéria Orgânica; Persistência de Pesticida; Qualidade; Solo. |
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Thesaurus NAL: |
Pesticides; Soil organic matter. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/205725/1/The-quality-of-soil-organic-matter-accessed-by-13C-Solid-State-NMR-2019.pdf
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Marc: |
LEADER 01604nam a2200217 a 4500
001 2115391
005 2019-12-17
008 2019 bl uuuu u00u1 u #d
100 1 $aNOVOTNY, E. H.
245 $aThe quality of soil organic matter, accessed by 13C Solid State NMR, is more important than its content concerning pesticide adsorption.$h[electronic resource]
260 $aIn: AUSTRALIAN AND NEW ZEALAND SOCIETY FOR MAGNETIC RESONANCE CONFERENCE, 12., 2019, Cape Naturaliste. Conference handbook. Randwick: Australian and New Zealand Society for Magnetic Resonance, 2019. p. 76. ANZMAG 2019.$c2019
520 $aThe need for food will increase by 59-98% from 2005 to 2050, and its global growth production has been achieved mainly through the intensive use of inputs such as pesticides and chemical fertilizers. Once released to the soil, sorption (represented by Kd values) and degradation are two governing processes that determine the distribution and persistence of pesticides. In spite of the huge dataset, the only apparent generalization is the high correlation between Kd and soil organic matter (SOM) content. This is because the SOM is the main adsorption site for pesticides. Seeking to normalize the experimental data and to access the pesticides mobility, the KOC value is calculated: Kd normalized by soil organic C content (C).
650 $aPesticides
650 $aSoil organic matter
650 $aMatéria Orgânica
650 $aPersistência de Pesticida
650 $aQualidade
650 $aSolo
700 1 $aTURETTA, A. P. D.
700 1 $aRESENDE, M. F. de
700 1 $aREBELLO, C. M.
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