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Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
25/01/2022 |
Data da última atualização: |
11/04/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CINTRA, L. A.; SOUZA, T. B. de; PARTEKA, L. M.; BARRETO, L. M.; PEREIRA, L. F. P.; GAETA, M. L.; GUYOT, R.; VANZELA, A. L. L. |
Afiliação: |
LEONARDO ADABO CINTRA, UNIVERSIDADE ESTADUAL DE LONDRINA; THAÍSSA BOLDIERI DE SOUZA, UNIVERSIDADE ESTADUAL DE LONDRINA; LETÍCIA MARIA PARTEKA, UNIVERSIDADE ESTADUAL DE LONDRINA; LUCAS MESQUITA BARRETO, UNIVERSIDADE ESTADUAL DE LONDRINA; LUIZ FILIPE PROTASIO PEREIRA, CNPCa; MARCOS LETAIF GAETA, UNIVERSIDADE ESTADUAL DE LONDRINA; ROMAIN GUYOT, CIRAD; ANDRÉ LUÍS LAFORGA VANZELA, UNIVERSIDADE ESTADUAL DE LONDRINA. |
Título: |
An 82 bp tandem repeat family typical of 3' non-coding end of Gypsy/TAT LTR retrotransposons is conserved in Coffea spp. pericentromeres. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Genome, v.65, n. 3, p. 137-181, 2021. |
DOI: |
https://doi.org/10.1139/gen-2021-0045 |
Idioma: |
Inglês |
Notas: |
Date of Electronic Publication: 2021 Nov 02. |
Conteúdo: |
Coffea spp. chromosomes are very small and accumulate a variety of repetitive DNA families around the centromeres. However, the proximal regions of Coffea chromosomes remain poorly understood, especially regarding the nature and organisation of the sequences. Taking advantage of the genome sequences of C. arabica (2n = 44), C. canephora, and C. eugenioides (C. arabica progenitors with 2n = 22) and good coverage genome sequencing of dozens of other wild Coffea spp., repetitive DNA sequences were identified, and the genomes were compared to decipher particularities of pericentromeric structures. The searches revealed a short tandem repeat (82 bp length) typical of Gypsy/TAT LTR retrotransposons, named Coffea_sat11. This repeat organises clusters with fragments of other transposable elements, comprising regions of non-coding RNA production. Cytogenomic analyses showed that Coffea_sat11 extends from the pericentromeres towards the middle of the chromosomal arms. This arrangement was observed in the allotetraploid C. arabica chromosomes, as well as in its progenitors. This study improves our understanding of the role of the Gypsy/TAT LTR retrotransposon lineage in the organisation of Coffea pericentromeres, as well as the conservation of Coffea_sat11 within the genus. The relationships between fragments of other transposable elements and the functional aspects of these sequences on the pericentromere chromatin were also evaluated. Highlights: A scattered short tandem repeat, typical of Gypsy/TAT LTR retrotransposons, associated with several fragments of other transposable elements, accumulates in the pericentromeres of Coffea chromosomes. This arrangement is preserved in all clades of the genus and appears to have a strong regulatory role in the organisation of chromatin around centromeres. MenosCoffea spp. chromosomes are very small and accumulate a variety of repetitive DNA families around the centromeres. However, the proximal regions of Coffea chromosomes remain poorly understood, especially regarding the nature and organisation of the sequences. Taking advantage of the genome sequences of C. arabica (2n = 44), C. canephora, and C. eugenioides (C. arabica progenitors with 2n = 22) and good coverage genome sequencing of dozens of other wild Coffea spp., repetitive DNA sequences were identified, and the genomes were compared to decipher particularities of pericentromeric structures. The searches revealed a short tandem repeat (82 bp length) typical of Gypsy/TAT LTR retrotransposons, named Coffea_sat11. This repeat organises clusters with fragments of other transposable elements, comprising regions of non-coding RNA production. Cytogenomic analyses showed that Coffea_sat11 extends from the pericentromeres towards the middle of the chromosomal arms. This arrangement was observed in the allotetraploid C. arabica chromosomes, as well as in its progenitors. This study improves our understanding of the role of the Gypsy/TAT LTR retrotransposon lineage in the organisation of Coffea pericentromeres, as well as the conservation of Coffea_sat11 within the genus. The relationships between fragments of other transposable elements and the functional aspects of these sequences on the pericentromere chromatin were also evaluated. Highlights: A scattered short tandem repeat, typi... Mostrar Tudo |
Thesaurus Nal: |
Chromosomes; Coffea; DNA probes; Genome. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02675naa a2200277 a 4500 001 2139296 005 2022-04-11 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1139/gen-2021-0045$2DOI 100 1 $aCINTRA, L. A. 245 $aAn 82 bp tandem repeat family typical of 3' non-coding end of Gypsy/TAT LTR retrotransposons is conserved in Coffea spp. pericentromeres.$h[electronic resource] 260 $c2021 500 $aDate of Electronic Publication: 2021 Nov 02. 520 $aCoffea spp. chromosomes are very small and accumulate a variety of repetitive DNA families around the centromeres. However, the proximal regions of Coffea chromosomes remain poorly understood, especially regarding the nature and organisation of the sequences. Taking advantage of the genome sequences of C. arabica (2n = 44), C. canephora, and C. eugenioides (C. arabica progenitors with 2n = 22) and good coverage genome sequencing of dozens of other wild Coffea spp., repetitive DNA sequences were identified, and the genomes were compared to decipher particularities of pericentromeric structures. The searches revealed a short tandem repeat (82 bp length) typical of Gypsy/TAT LTR retrotransposons, named Coffea_sat11. This repeat organises clusters with fragments of other transposable elements, comprising regions of non-coding RNA production. Cytogenomic analyses showed that Coffea_sat11 extends from the pericentromeres towards the middle of the chromosomal arms. This arrangement was observed in the allotetraploid C. arabica chromosomes, as well as in its progenitors. This study improves our understanding of the role of the Gypsy/TAT LTR retrotransposon lineage in the organisation of Coffea pericentromeres, as well as the conservation of Coffea_sat11 within the genus. The relationships between fragments of other transposable elements and the functional aspects of these sequences on the pericentromere chromatin were also evaluated. Highlights: A scattered short tandem repeat, typical of Gypsy/TAT LTR retrotransposons, associated with several fragments of other transposable elements, accumulates in the pericentromeres of Coffea chromosomes. This arrangement is preserved in all clades of the genus and appears to have a strong regulatory role in the organisation of chromatin around centromeres. 650 $aChromosomes 650 $aCoffea 650 $aDNA probes 650 $aGenome 700 1 $aSOUZA, T. B. de 700 1 $aPARTEKA, L. M. 700 1 $aBARRETO, L. M. 700 1 $aPEREIRA, L. F. P. 700 1 $aGAETA, M. L. 700 1 $aGUYOT, R. 700 1 $aVANZELA, A. L. L. 773 $tGenome$gv.65, n. 3, p. 137-181, 2021.
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Embrapa Café (CNPCa) |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
28/05/1998 |
Data da última atualização: |
17/10/2023 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
VASCONCELOS, V. R.; RESENDE, K. T.; PIMENTEL, J. C. M.; CARVALHO, F. F. R. de; RIBEIRO, V. Q.; DORIGAN, C. J. |
Afiliação: |
VÂNIA RODRIGUES VASCONCELOS, CNPC; KLÉBER TOMÁS DE RESENDE, UNESP - Jaboticabal, SP; JOSÉ CARLOS MACHADO PIMENTEL, CNPC; FRANCISCO FERNANDO F. DE CARVALHO, Pós-graduação - UNESP - Jaboticabal, SP; VALDENIR QUEIROZ RIBEIRO, CNPC; CLÁUDIA JOSEFINA DORIGAN, Pós-graduação - UNESP - Jaboticabal, SP. |
Título: |
Cinética de degradação ruminal da proteína de forrageiras do semi-árido brasileiro em caprinos. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora. Anais... Juiz de Fora: Sociedade Brasileira de Zootecnia, 1997. v. 1, p. 52-54. |
Idioma: |
Português |
Conteúdo: |
Resumo: Determinou-se as diferentes degradabilidades da proteina de acordo com suas atividades de degradacao e passagem calculadas segundo metodologia proposta pelo AFRC (1995), utilizando-se as fracoes soluveis(A), potencialmente degradavel(B), indegradavel(C) e a taxa de degradacao da fracao B (C), parametros da equacao de ORSKOV & McDONALD (1979). A concentracao de proteina bruta e do nitrogenio insoluvel em detergente acido foram determinados segundo SILVA (1990). Os fenos de sabia (Mimosa caesalpinifolia) e jurema preta (Mimosa atenuifolia) apresentaram baixo potencial de degradacao ruminal da PB e os fenos de catingueira (Caesalpinia bracteosa) e leucena (Leucaena leucocephala) percentuais elevados de proteina degradada no rumen, podendo ser usadas como suplementos proteicos na regiao semi-arida no Nordeste. [Cinetic of rumenal degradation of the protein of brazilian semi-arid forages in goats]. |
Palavras-Chave: |
Brasil; Caesalpinia bracteosa; Ceará; Degradabilidade; Degradability; Mimosa atenuifolia; Mimosa caesalpinifolia; Protein. |
Thesagro: |
Caprino; Catingueira; Feno; Jurema preta; Leucaena; Leucaena leucocephala; Proteina; Sabiá. |
Thesaurus NAL: |
Brazil; Goats; Hay; Semiarid zones. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/48382/1/AAC-Cinetica-de-degradacao.pdf
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Marc: |
LEADER 02131nam a2200409 a 4500 001 1514952 005 2023-10-17 008 1997 bl uuuu u00u1 u #d 100 1 $aVASCONCELOS, V. R. 245 $aCinética de degradação ruminal da proteína de forrageiras do semi-árido brasileiro em caprinos.$h[electronic resource] 260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora. Anais... Juiz de Fora: Sociedade Brasileira de Zootecnia, 1997. v. 1, p. 52-54.$c1997 520 $aResumo: Determinou-se as diferentes degradabilidades da proteina de acordo com suas atividades de degradacao e passagem calculadas segundo metodologia proposta pelo AFRC (1995), utilizando-se as fracoes soluveis(A), potencialmente degradavel(B), indegradavel(C) e a taxa de degradacao da fracao B (C), parametros da equacao de ORSKOV & McDONALD (1979). A concentracao de proteina bruta e do nitrogenio insoluvel em detergente acido foram determinados segundo SILVA (1990). Os fenos de sabia (Mimosa caesalpinifolia) e jurema preta (Mimosa atenuifolia) apresentaram baixo potencial de degradacao ruminal da PB e os fenos de catingueira (Caesalpinia bracteosa) e leucena (Leucaena leucocephala) percentuais elevados de proteina degradada no rumen, podendo ser usadas como suplementos proteicos na regiao semi-arida no Nordeste. [Cinetic of rumenal degradation of the protein of brazilian semi-arid forages in goats]. 650 $aBrazil 650 $aGoats 650 $aHay 650 $aSemiarid zones 650 $aCaprino 650 $aCatingueira 650 $aFeno 650 $aJurema preta 650 $aLeucaena 650 $aLeucaena leucocephala 650 $aProteina 650 $aSabiá 653 $aBrasil 653 $aCaesalpinia bracteosa 653 $aCeará 653 $aDegradabilidade 653 $aDegradability 653 $aMimosa atenuifolia 653 $aMimosa caesalpinifolia 653 $aProtein 700 1 $aRESENDE, K. T. 700 1 $aPIMENTEL, J. C. M. 700 1 $aCARVALHO, F. F. R. de 700 1 $aRIBEIRO, V. Q. 700 1 $aDORIGAN, C. J.
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