|
|
Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
03/12/2014 |
Data da última atualização: |
03/12/2014 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BEVITORI, R.; OLIVEIRA, M. B.; GROSSI-DE-SÁ, M. F.; LANNA, A. C.; SILVEIRA, R. D. da; PETROFEZA, S. |
Afiliação: |
ROSANGELA BEVITORI, CNPAF; MARILIA BARROS OLIVEIRA, doutoranda UFG; MARIA FATIMA GROSSI DE SA, CENARGEN; ANNA CRISTINA LANNA, CNPAF; RICARDO DIÓGENES DA SILVEIRA; SILVANA PETROFEZA, UFG. |
Título: |
Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 13, n. 4, p. 9795-9805, 2014. |
ISSN: |
1676-5680 |
DOI: |
http://dx.doi.org/10.4238/2014.November.27.7 |
Idioma: |
Inglês |
Conteúdo: |
Drought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice. |
Thesagro: |
Arroz; Brusone; Doença de planta; Genética molecular; Oryza sativa; Seca. |
Thesaurus Nal: |
Blast disease; Gene expression; Magnaporthe oryzae; Rice. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112900/1/gmr4195.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179634/1/gmr4195.pdf
|
Marc: |
LEADER 02118naa a2200325 a 4500 001 2001427 005 2014-12-03 008 2014 bl uuuu u00u1 u #d 022 $a1676-5680 024 7 $ahttp://dx.doi.org/10.4238/2014.November.27.7$2DOI 100 1 $aBEVITORI, R. 245 $aSelection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought.$h[electronic resource] 260 $c2014 520 $aDrought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice. 650 $aBlast disease 650 $aGene expression 650 $aMagnaporthe oryzae 650 $aRice 650 $aArroz 650 $aBrusone 650 $aDoença de planta 650 $aGenética molecular 650 $aOryza sativa 650 $aSeca 700 1 $aOLIVEIRA, M. B. 700 1 $aGROSSI-DE-SÁ, M. F. 700 1 $aLANNA, A. C. 700 1 $aSILVEIRA, R. D. da 700 1 $aPETROFEZA, S. 773 $tGenetics and Molecular Research$gv. 13, n. 4, p. 9795-9805, 2014.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
12/07/1999 |
Data da última atualização: |
12/07/1999 |
Autoria: |
COSTA, C. N.; MILAGRES, J. C.; SILVA, M. de A. e; GARCIA, J. A.; CASTRO, A. C. G.; REIS, A. N. dos. |
Afiliação: |
Embrapa Suinos e Aves, Concordia, SC. |
Título: |
Fatores geneticos e de meio na producao de leite e eficiencia reprodutiva de um rebanho Holandes. |
Ano de publicação: |
1981 |
Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 18., 1981, Goiania, GO. Anais... Goiania: SBZ, 1981. |
Páginas: |
p.276 |
Idioma: |
Português |
Palavras-Chave: |
Gado de leite; Production. |
Thesagro: |
Meio Ambiente; Produção. |
Thesaurus NAL: |
dairy cattle; environment. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00765naa a2200253 a 4500 001 1434096 005 1999-07-12 008 1981 bl uuuu u00u1 u #d 100 1 $aCOSTA, C. N. 245 $aFatores geneticos e de meio na producao de leite e eficiencia reprodutiva de um rebanho Holandes. 260 $c1981 300 $ap.276 650 $adairy cattle 650 $aenvironment 650 $aMeio Ambiente 650 $aProdução 653 $aGado de leite 653 $aProduction 700 1 $aMILAGRES, J. C. 700 1 $aSILVA, M. de A. e 700 1 $aGARCIA, J. A. 700 1 $aCASTRO, A. C. G. 700 1 $aREIS, A. N. dos 773 $tIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 18., 1981, Goiania, GO. Anais... Goiania: SBZ, 1981.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|