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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
21/06/2024 |
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Data da última atualização: |
31/07/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COATRINI-SOARES, A.; SOARES, J. C.; POPOLIN-NETO, M.; MELLO, S. S. de; SANCHES, E. A.; PAULOVICH, F. V.; OLIVEIRA JR, O. N.; MATTOSO, L. H. C. |
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Afiliação: |
UNIVERSITY OF SÃO PAULO (USP); UNIVERSITY OF SAO PAULO (USP); UNIVERSITY OF SAO PAULO (USP); MASSACHUSETTS EYE AND EAR, HARVARD MEDICAL SCHOOL; FEDERAL UNIVERSITY OF AMAZONAS; EINDHOVEN UNIVERSITY OF TECHNOLOGY (TU/E); UNIVERSITY OF SAO PAULO (USP); LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
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Título: |
Multidimensional calibration spaces in Staphylococcus Aureus detection using chitosan-based genosensors and electronic tongue. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
International Journal of Biological Macromolecules, v. 271, 132460, 2024. |
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Páginas: |
1 - 10 |
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ISSN: |
0141-8130 |
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DOI: |
https://doi.org/10.1016/j.ijbiomac.2024.132460 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT Mastitis diagnosis can be made by detecting Staphylococcus aureus (S. aureus), which requires high sensitivity and selectivity. Here, we report on microfluidic genosensors and electronic tongues to detect S. aureus DNA using impedance spectroscopy with data analysis employing visual analytics and machine learning techniques. The genosensors were made with layer-by-layer films containing either 10 bilayers of chitosan/chondroitin sulfate or 8 bilayers of chitosan/sericin functionalized with an active layer of cpDNA S. aureus. The specific interactions leading to hybridization in these genosensors allowed for a low limit of detection of 5.90 × 10− 19 mol/L. The electronic tongue had four sensing units made with 6-bilayer chitosan/chondroitin sulfate films, 10-bilayer chitosan/chondroitin sulfate, 8-bilayer chitosan/sericin, and 8-bilayer chitosan/gold nanoparticles modified with sericin. Despite the absence of specific interactions, various concentrations of DNA S. aureus could be distinguished when the impedance data were plotted using a dimensionality reduction technique. Selectivity of S. aureus DNA was confirmed using multidimensional calibration spaces, based on machine learning, with accuracy up to 89 % for the genosensors and 66 % for the electronic tongue. Hence, with these computational methods one may opt for the more expensive genosensors or the simpler and cheaper electronic tongue, depending on the sensitivity level required to diagnose mastitis. |
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Palavras-Chave: |
Genosensors; Multidimensional calibration space. |
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Categoria do assunto: |
-- |
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Marc: |
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024 7 $ahttps://doi.org/10.1016/j.ijbiomac.2024.132460$2DOI
100 1 $aCOATRINI-SOARES, A.
245 $aMultidimensional calibration spaces in Staphylococcus Aureus detection using chitosan-based genosensors and electronic tongue.$h[electronic resource]
260 $c2024
300 $a1 - 10
520 $aABSTRACT Mastitis diagnosis can be made by detecting Staphylococcus aureus (S. aureus), which requires high sensitivity and selectivity. Here, we report on microfluidic genosensors and electronic tongues to detect S. aureus DNA using impedance spectroscopy with data analysis employing visual analytics and machine learning techniques. The genosensors were made with layer-by-layer films containing either 10 bilayers of chitosan/chondroitin sulfate or 8 bilayers of chitosan/sericin functionalized with an active layer of cpDNA S. aureus. The specific interactions leading to hybridization in these genosensors allowed for a low limit of detection of 5.90 × 10− 19 mol/L. The electronic tongue had four sensing units made with 6-bilayer chitosan/chondroitin sulfate films, 10-bilayer chitosan/chondroitin sulfate, 8-bilayer chitosan/sericin, and 8-bilayer chitosan/gold nanoparticles modified with sericin. Despite the absence of specific interactions, various concentrations of DNA S. aureus could be distinguished when the impedance data were plotted using a dimensionality reduction technique. Selectivity of S. aureus DNA was confirmed using multidimensional calibration spaces, based on machine learning, with accuracy up to 89 % for the genosensors and 66 % for the electronic tongue. Hence, with these computational methods one may opt for the more expensive genosensors or the simpler and cheaper electronic tongue, depending on the sensitivity level required to diagnose mastitis.
653 $aGenosensors
653 $aMultidimensional calibration space
700 1 $aSOARES, J. C.
700 1 $aPOPOLIN-NETO, M.
700 1 $aMELLO, S. S. de
700 1 $aSANCHES, E. A.
700 1 $aPAULOVICH, F. V.
700 1 $aOLIVEIRA JR, O. N.
700 1 $aMATTOSO, L. H. C.
773 $tInternational Journal of Biological Macromolecules$gv. 271, 132460, 2024.
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| 1. |  | AMDREAZZI, M. A.; MORAES, G. V. de; SANTOS, G. T. dos; MARTINS, E. N.; MACEDO, F. de A. F. de; REIS, A. C. F. dos. Efeito do caroco de algodao sobre o desenvolvimento e desempenho reprodutivo de caprinos machos. Arquivos de Biologia e Tecnologia, v. 38, n. 3, p. 799-813, Set., 1995.| Biblioteca(s): Embrapa Meio-Norte. |
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