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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
23/10/2003 |
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Data da última atualização: |
31/05/2007 |
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Autoria: |
PINHEIRO, R. de O.; BODDEY, L. H.; JAMES, A. K.; SPRENT, J. I.; BODDEY, R. M. |
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Título: |
Adsorption and anchoring of Azospirillum strains to roots of wheat seedlings. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Pant and Soil, Netherlands, v. 246, p. 151-166, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Recent microscopic evidence acquired using strain-specific monoclonal antibodies and specific gene probes confirms earlier claims that some strains of Azospirillum lipoferum and A. brasilense, but not others, are capable of infecting the interior of wheat roots. The present study was performed to determine whether this strain specificity in the infection of the interior of wheat roots was apparent in the first 24 h of adsorption ('anchoring') of Azospiri1lum cells to the root surface. Strains of A. brasilense, originally isolated from surface-sterilized wheat roots (Sp 245, Sp 107) or with a proven ability to infect the interior of wheat roots (Sp 245), showed no greater ability to anchor to the roots than other Azospirillum strains isolated from the wheat rhizosphere (Sp 246) or from the rhizosphere or rhizosphere soil of other gramineae (Sp 7, Cd, S 82). The SEM images showed that at the root tip the Azospirillum cells were principally located in cracks between epidermal cells. In the root hair zone the bacteria were more numerous but again principally located in the depressions between epidermal cells. In all zones of the roots mucilage was present, and near the tip this appeared to have been partially digested, forming 'halos' aroun4 the bacteria and revealing fibril-like strands attached to the bacteria. Subsequent studies were conducted using à technique originally developed for investigating competition of rhizobia for adsorption sites on legume roots. In the adaptation of this technique it was found that the presence of any significant concentration of Ca++ in the incubation medium reduced bacterial adsorption, as did concentrations of (PO4)3- above 50 m M. The influence of the pH of the incubation medium on the adsorption of ten different strains of Azospirillum showed, that with one exception, strains isolated from the roots or rhizosphere of wheat showed optimum adsorption at pH 6.0, and all other strains pH 7.0. Apart from this effect of pH no differences in adsorption were detected between strains with a proven capacity to infect wheat roots and those unable to do so. However, strains varied in their capability to compete for adsorption sites, there being a tendency for strains with a proven capacity to invade the internal tissues of wheat roots to be more competitive for adsorption sites. MenosRecent microscopic evidence acquired using strain-specific monoclonal antibodies and specific gene probes confirms earlier claims that some strains of Azospirillum lipoferum and A. brasilense, but not others, are capable of infecting the interior of wheat roots. The present study was performed to determine whether this strain specificity in the infection of the interior of wheat roots was apparent in the first 24 h of adsorption ('anchoring') of Azospiri1lum cells to the root surface. Strains of A. brasilense, originally isolated from surface-sterilized wheat roots (Sp 245, Sp 107) or with a proven ability to infect the interior of wheat roots (Sp 245), showed no greater ability to anchor to the roots than other Azospirillum strains isolated from the wheat rhizosphere (Sp 246) or from the rhizosphere or rhizosphere soil of other gramineae (Sp 7, Cd, S 82). The SEM images showed that at the root tip the Azospirillum cells were principally located in cracks between epidermal cells. In the root hair zone the bacteria were more numerous but again principally located in the depressions between epidermal cells. In all zones of the roots mucilage was present, and near the tip this appeared to have been partially digested, forming 'halos' aroun4 the bacteria and revealing fibril-like strands attached to the bacteria. Subsequent studies were conducted using à technique originally developed for investigating competition of rhizobia for adsorption sites on legume roots. In the adaptati... Mostrar Tudo |
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Palavras-Chave: |
Wheats. |
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Thesagro: |
Trigo. |
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Thesaurus Nal: |
Azospirillum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02896naa a2200205 a 4500
001 1624717
005 2007-05-31
008 2002 bl --- 0-- u #d
100 1 $aPINHEIRO, R. de O.
245 $aAdsorption and anchoring of Azospirillum strains to roots of wheat seedlings.
260 $c2002
520 $aRecent microscopic evidence acquired using strain-specific monoclonal antibodies and specific gene probes confirms earlier claims that some strains of Azospirillum lipoferum and A. brasilense, but not others, are capable of infecting the interior of wheat roots. The present study was performed to determine whether this strain specificity in the infection of the interior of wheat roots was apparent in the first 24 h of adsorption ('anchoring') of Azospiri1lum cells to the root surface. Strains of A. brasilense, originally isolated from surface-sterilized wheat roots (Sp 245, Sp 107) or with a proven ability to infect the interior of wheat roots (Sp 245), showed no greater ability to anchor to the roots than other Azospirillum strains isolated from the wheat rhizosphere (Sp 246) or from the rhizosphere or rhizosphere soil of other gramineae (Sp 7, Cd, S 82). The SEM images showed that at the root tip the Azospirillum cells were principally located in cracks between epidermal cells. In the root hair zone the bacteria were more numerous but again principally located in the depressions between epidermal cells. In all zones of the roots mucilage was present, and near the tip this appeared to have been partially digested, forming 'halos' aroun4 the bacteria and revealing fibril-like strands attached to the bacteria. Subsequent studies were conducted using à technique originally developed for investigating competition of rhizobia for adsorption sites on legume roots. In the adaptation of this technique it was found that the presence of any significant concentration of Ca++ in the incubation medium reduced bacterial adsorption, as did concentrations of (PO4)3- above 50 m M. The influence of the pH of the incubation medium on the adsorption of ten different strains of Azospirillum showed, that with one exception, strains isolated from the roots or rhizosphere of wheat showed optimum adsorption at pH 6.0, and all other strains pH 7.0. Apart from this effect of pH no differences in adsorption were detected between strains with a proven capacity to infect wheat roots and those unable to do so. However, strains varied in their capability to compete for adsorption sites, there being a tendency for strains with a proven capacity to invade the internal tissues of wheat roots to be more competitive for adsorption sites.
650 $aAzospirillum
650 $aTrigo
653 $aWheats
700 1 $aBODDEY, L. H.
700 1 $aJAMES, A. K.
700 1 $aSPRENT, J. I.
700 1 $aBODDEY, R. M.
773 $tPant and Soil, Netherlands$gv. 246, p. 151-166, 2002.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
24/07/2008 |
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Data da última atualização: |
07/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
AMARAL, J. A. B. do; AQUINO, M. de; BAMBINI, M. D.; BASTOS, M. do S. R.; CALEGARIO, F. F.; COHEN, R. S.; COUTINHO, W. M.; CRIPPA, M. E. N.; DANTAS, N. S. S.; FIGUEIREDO, R. A.; FOLEGATTI, M. I. da S.; GALVANI, F.; GUEDES, V. G. F.; HANASHIRO, M. M.; KITAMURA, P. C.; LUCHIARI JUNIOR, A.; MAESTRO, A.; MARION, M. L. P.; MENDES, M. D. L.; MOURAO, E. A. B.; MORICONI, W.; MARTINS NETTO, D. A.; PICCHI, C. M. C.; PINHEIRO, R. R.; REIS, A. E. G. dos; RUMJANEK, N. G.; SITTON, M.; SPADOTTO, C. A.; VIEIRA, J. L. G.; VINHOLIS, M. de M. B.; ZIMMER, L. E.; AFONSO JUNIOR, P. C.; LEITAO, J. S. de S.; SANTOS, G. C. dos. |
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Afiliação: |
JOSE AMERICO BORDINI DO AMARAL, CNPAT; MOISES DE AQUINO, CNPSo; MARTHA DELPHINO BAMBINI, CNPTIA; MARIA DO SOCORRO ROCHA BASTOS, CNPAT; FAGONI FAYER CALEGARIO, CNPMA; RICARDO SOARES COHEN, SGE; WIRTON MACEDO COUTINHO, CNPA; MARGARETE ESTEVES NUNES CRIPPA, CNPMA; NILO SERGIO SILVA DANTAS, CPATC; RICARDO ALAMINO FIGUEIREDO, CENARGEN; MARILIA IEDA DA SILVEIRA FOLEGATTI, DPD; FABIO GALVANI, CPAP; VICENTE GALILEU FERREIRA GUEDES, SGE; MARCELO MIKIO HANASHIRO, CNPTIA; PAULO CHOJI KITAMURA, CNPMA; ARIOVALDO LUCHIARI JUNIOR, CNPMA; ADILSON MAESTRO; MIRIAM LUCIA PIMENTEL MARION, CNPMA; MARA DENISE LUCK MENDES, CNPMA; ELESSANDRA APARECIDA BENTO MOURAO, SAPC; WALDEMORE MORICONI, CNPMA; DEA ALECIA MARTINS NETTO, CNPMS; CRISTINA MARIA CIRINO PICCHI, CPPSE; RAYMUNDO RIZALDO PINHEIRO, CNPC; ANTONIO EDUARDO GUIMARAES DOS REIS, SGE; NORMA GOUVEA RUMJANEK, CNPAB; MELISSA SITTON, CNPMA; CLAUDIO APARECIDO SPADOTTO, CNPMA; JOB LUCIO GOMES VIEIRA, SGE; MARCELA DE MELLO BRANDAO VINHOLIS, CPPSE; LORIEN ELIANE ZIMMER, CNPSA; PAULO CESAR AFONSO JUNIOR, SAPC; JACQUELINE SILVEIRA DE SA LEITAO, SGE; GERALDO CARDOSO DOS SANTOS, SGE. |
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Título: |
ISOEMBRAPA - Perspectivas na busca pela excelência na gestão da Embrapa. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO DA ASSOCIAÇÃO BRASILEIRA DE PESQUISA TECNOLÓGICA, 2008, Campina Grande. Os desníveis regionais e a inovação no Brasil: os desafios para as instituições de pesquisa tecnológica: resumos. Brasília, DF: ABIPTI, 2008. |
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Páginas: |
p. 68-69. |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho tem o objetivo de apresentar os primeiros resultados deste projeto, oriundos de um diagnóstico inicial, relativo às ações para promoção da melhoria de processos internos, realizado junto às unidades participantes do ISOEMBRAPA. |
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Palavras-Chave: |
Embrapa. |
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Thesagro: |
Gestão; Gestão do Conhecimento. |
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Thesaurus NAL: |
Knowledge. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02014nam a2200565 a 4500
001 1008656
005 2020-02-07
008 2008 bl uuuu u01u1 u #d
100 1 $aAMARAL, J. A. B. do
245 $aISOEMBRAPA - Perspectivas na busca pela excelência na gestão da Embrapa.$h[electronic resource]
260 $aIn: CONGRESSO DA ASSOCIAÇÃO BRASILEIRA DE PESQUISA TECNOLÓGICA, 2008, Campina Grande. Os desníveis regionais e a inovação no Brasil: os desafios para as instituições de pesquisa tecnológica: resumos. Brasília, DF: ABIPTI$c2008
300 $ap. 68-69.
520 $aEste trabalho tem o objetivo de apresentar os primeiros resultados deste projeto, oriundos de um diagnóstico inicial, relativo às ações para promoção da melhoria de processos internos, realizado junto às unidades participantes do ISOEMBRAPA.
650 $aKnowledge
650 $aGestão
650 $aGestão do Conhecimento
653 $aEmbrapa
700 1 $aAQUINO, M. de
700 1 $aBAMBINI, M. D.
700 1 $aBASTOS, M. do S. R.
700 1 $aCALEGARIO, F. F.
700 1 $aCOHEN, R. S.
700 1 $aCOUTINHO, W. M.
700 1 $aCRIPPA, M. E. N.
700 1 $aDANTAS, N. S. S.
700 1 $aFIGUEIREDO, R. A.
700 1 $aFOLEGATTI, M. I. da S.
700 1 $aGALVANI, F.
700 1 $aGUEDES, V. G. F.
700 1 $aHANASHIRO, M. M.
700 1 $aKITAMURA, P. C.
700 1 $aLUCHIARI JUNIOR, A.
700 1 $aMAESTRO, A.
700 1 $aMARION, M. L. P.
700 1 $aMENDES, M. D. L.
700 1 $aMOURAO, E. A. B.
700 1 $aMORICONI, W.
700 1 $aMARTINS NETTO, D. A.
700 1 $aPICCHI, C. M. C.
700 1 $aPINHEIRO, R. R.
700 1 $aREIS, A. E. G. dos
700 1 $aRUMJANEK, N. G.
700 1 $aSITTON, M.
700 1 $aSPADOTTO, C. A.
700 1 $aVIEIRA, J. L. G.
700 1 $aVINHOLIS, M. de M. B.
700 1 $aZIMMER, L. E.
700 1 $aAFONSO JUNIOR, P. C.
700 1 $aLEITAO, J. S. de S.
700 1 $aSANTOS, G. C. dos
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