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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
15/07/2002 |
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Data da última atualização: |
30/03/2017 |
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Autoria: |
VENTURA, R. M.; TAKATA, C. S. A.; SILVA, R. A. M. S.; NUNES, V. L.; TAKEDA, G. F.; TEIXEIRA, M. M. G. |
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Afiliação: |
USP/DP/ICB (Sao Paulo, SP). EMBRAPA Suinos e Aves (Concordia, SC). |
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Título: |
Molecular and morphological studies of Brazilian Trypanosoma evansi stocks: the total absence of kDNA in Trypanosomes from both laboratory stocks and naturally infected domestic and wild mammals. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Journal of Parasitology, v.86, n.6, p.1289-1298, Dec. 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
The kinetoplast DNA (kDNA) minircle molecules of 14 Brazilian stocks of Trypanosoma evansi were studied by morphological approaches (Giemsa and 4'-6'-diamidino-2-phenylin-
dole staining and transmission electyron microscopy) and molecular approaches (probing with an oligonucleotide complementary to the minicircle origin of replication and polymerase chain reaction amplification of a minicircle sequence). All methods indicated the absense of both a typical kinetoplast and kDNA minicircles, even in a very small number of parasites of a single stock or in small numbers of copies of molecules per cell. We did not detect any altered kDNA molecules. There were no KDNA molecules in eithger old or new
stocks of T. evansi maintained by successive passages in mice. Similarly, no kDNA minicircles were detected in trypanosomes in blood smears from naturally infected domestic and wild animals. Thus, the total absence of KDNA in Brazilian stocks of T. evansi from both domestic and wild mammals is probably the natural state of Brazilian T. evansi. |
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Palavras-Chave: |
Domestic animal; Infeccao natural; Mamifero silvestre; Molecular; Morfologia; Morphology; Naturally infected; Parasita; Parasite; Wild mammal. |
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Thesagro: |
Animal Domestico. |
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Thesaurus Nal: |
Brazil; Trypanosoma evansi. |
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Categoria do assunto: |
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Marc: |
LEADER 02073naa a2200337 a 4500
001 1810175
005 2017-03-30
008 2000 bl --- 0-- u #d
100 1 $aVENTURA, R. M.
245 $aMolecular and morphological studies of Brazilian Trypanosoma evansi stocks$bthe total absence of kDNA in Trypanosomes from both laboratory stocks and naturally infected domestic and wild mammals.
260 $c2000
520 $aThe kinetoplast DNA (kDNA) minircle molecules of 14 Brazilian stocks of Trypanosoma evansi were studied by morphological approaches (Giemsa and 4'-6'-diamidino-2-phenylin- dole staining and transmission electyron microscopy) and molecular approaches (probing with an oligonucleotide complementary to the minicircle origin of replication and polymerase chain reaction amplification of a minicircle sequence). All methods indicated the absense of both a typical kinetoplast and kDNA minicircles, even in a very small number of parasites of a single stock or in small numbers of copies of molecules per cell. We did not detect any altered kDNA molecules. There were no KDNA molecules in eithger old or new stocks of T. evansi maintained by successive passages in mice. Similarly, no kDNA minicircles were detected in trypanosomes in blood smears from naturally infected domestic and wild animals. Thus, the total absence of KDNA in Brazilian stocks of T. evansi from both domestic and wild mammals is probably the natural state of Brazilian T. evansi.
650 $aBrazil
650 $aTrypanosoma evansi
650 $aAnimal Domestico
653 $aDomestic animal
653 $aInfeccao natural
653 $aMamifero silvestre
653 $aMolecular
653 $aMorfologia
653 $aMorphology
653 $aNaturally infected
653 $aParasita
653 $aParasite
653 $aWild mammal
700 1 $aTAKATA, C. S. A.
700 1 $aSILVA, R. A. M. S.
700 1 $aNUNES, V. L.
700 1 $aTAKEDA, G. F.
700 1 $aTEIXEIRA, M. M. G.
773 $tJournal of Parasitology$gv.86, n.6, p.1289-1298, Dec. 2000.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/01/2017 |
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Data da última atualização: |
25/01/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ANDRADE, L. V. de; REINHARDT, D. H. R. C. |
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Afiliação: |
LEANDRO VIDAL DE ANDRADE, FAPESB; DOMINGO HAROLDO RUDOLFO C REINHARDT, CNPMF. |
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Título: |
Pesquisa e levantamento de dados que visam auxiliar ao mapeamento da fruticultura do estado da Bahia. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 10., 2016: Cruz das Almas, BA. Traduzindo ciência para o mundo : resumos. Brasília, DF : Embrapa, 2016. |
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Idioma: |
Português |
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Thesagro: |
Fruticultura. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/153907/1/AnaisJornada-2016-11.pdf
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Marc: |
LEADER 00543nam a2200121 a 4500
001 2061659
005 2017-01-25
008 2016 bl uuuu u00u1 u #d
100 1 $aANDRADE, L. V. de
245 $aPesquisa e levantamento de dados que visam auxiliar ao mapeamento da fruticultura do estado da Bahia.$h[electronic resource]
260 $aIn: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 10., 2016: Cruz das Almas, BA. Traduzindo ciência para o mundo : resumos. Brasília, DF : Embrapa$c2016
650 $aFruticultura
700 1 $aREINHARDT, D. H. R. C.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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