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Registros recuperados : 77 | |
23. | | CUNHA, N. B. da; MURAD, A. M.; VIANNA, G. R.; RECH FILHO, E. L. Recombinant biosynthesis of functional human growth hormone and coagulation factor IX in transgenic soybean seeds. BMC Proceedings, v.8, suppl, P.112, 2014. (Open access) Edição do Congress of the Brazilian Biotechnology Society, Florianópolis, 2013. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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25. | | LIMA, T. A.; SILVA, D. R.; RECH FILHO, E. L.; LACORTE, C. C. Produção heteróloga de Cianovirina-N em culturas de células em suspensão de fumo. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 14., 2009, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2009. Resumo 016. p. 50 Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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27. | | LACORTE, C. C.; MURAD, A. M.; MADEIRA, L.; CUNHA, N. B.; RECH FILHO, E. L. Subcellular targeting of recombinant Cyanovirin-N, An-HIV microbiocide, in soybean and Nicotiana bethamiana plants. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 2., 2009, Búzios. Programa e resumos. [s.l.]: Sociedade Brasileira de Genética, 2009. p. 252. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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30. | | MURAD, A. M.; CUNHA, N.; LACORTE, C. C.; COELHO, M. C. F.; VIANNA, G. R.; RECH FILHO, E. L. Expression, purification and analysis of the anti-HIV Cyanovirin-N produced in transgenic soybeans seeds. BMC Proceedings, v.8, suppl, P.105, 2014. (Open access) Edição do Congress of the Brazilian Biotechnology Society, Florianópolis, 2013. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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33. | | MACHADO, A.; MURAD, A. M.; VIANNA, G. R.; CUNHA, N.; COELHO, C.; COELHO, M. C. F.; RECH FILHO, E. L. Metabolic engineering of fatty acids from soybean seeds. BMC Proceedings, v.8, suppl, P.93, 2014. (Open access) Edição do Congress of the Brazilian Biotechnology Society, Florianópolis, 2013. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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34. | | PROSDOCIMI, F.; BITTENCOURT, D. M. de C.; SILVA, F. R. D.; MOTTA, P. C.; RECH FILHO, E. L. Molecular, behavioral and anatomical sophistication in spider webs: insights from spinning gland RNA-seq experiments in primitive and modern spiders. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 6., 2010, Ouro Preto. Abstracts... [S.l.: s.n.], 2010. p. 67. Biblioteca(s): Embrapa Amazônia Ocidental. |
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36. | | TEULÉ, F.; COOPER, A. R.; FURIN, W. A.; BITTENCOURT, D.; RECH FILHO, E. L.; BROOKS, A.; LEWIS, R. V. A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning. Nature Protocols, v. 4, n. 3, p. 341-355, fev. 2009. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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37. | | MANFRÃO-NETTO, J. H. C.; QUEIROZ, E. B.; RODRIGUES, K. A.; COELHO, C. M.; PAES, H. C.; RECH FILHO, E. L.; PARACHIN, N. S. Evaluation of Ogataea (Hansenula) polymorpha for hyaluronic acid production. Microorganisms, v. 9, 312, 2021. Na publicação: Elibio Leopoldo Rech. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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39. | | SILVA JUNIOR, O. B. da; PESSOA FILHO, M. A. C. de P.; SA, M. E. L.; DEAL, R.; RAGOUSSIS, I.; GRATTAPAGLIA, D.; RECH FILHO, E. L. Coupling in-depth genome annotations with genome editing technology for harnessing genomic variation to promote precision breeding in tropical soybean. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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40. | | SILVA JUNIOR, O. B. da; PESSOA FILHO, M. A. C. de P.; SA, M. E. L.; DEAL, R.; RAGOUSSIS, I.; GRATTAPAGLIA, D.; RECH FILHO, E. L. Coupling in-depth genome annotations with genome editing technology for harnessing genomic variation to promote precision breeding in tropical soybean. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018. Biblioteca(s): Embrapa Cerrados. |
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Registros recuperados : 77 | |
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Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
29/11/2018 |
Data da última atualização: |
29/11/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVA JUNIOR, O. B. da; PESSOA FILHO, M. A. C. de P.; SA, M. E. L.; DEAL, R.; RAGOUSSIS, I.; GRATTAPAGLIA, D.; RECH FILHO, E. L. |
Afiliação: |
ORZENIL BONFIM DA SILVA JUNIOR, Cenargen; MARCO AURELIO CALDAS DE PINHO PESSO, CPAC; Cenargen; EMORY UNIVERSITY; MCGILL UNIVERSITY; DARIO GRATTAPAGLIA, Cenargen; ELIBIO LEOPOLDO RECH FILHO, Cenargen. |
Título: |
Coupling in-depth genome annotations with genome editing technology for harnessing genomic variation to promote precision breeding in tropical soybean. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018. |
Idioma: |
Inglês |
Conteúdo: |
ABSTRACT: Directional selection during crop domestication and advanced breeding has resulted in significant changes in plant genomes. The extent of intraspecific variation in a crop documents domestication and highlights variants underlying complex traits. Studies demonstrate that most naturally occurring variants in crops are low frequency, and only a small fraction of those might have important functions to trait variation. To understand the inheritance of quantitative traits, diversity panels have become available by crossing inbred lines to produce genomic maps that relate phenotypic variation to recombination and ultimately to genome structure. Using these resources, genomics studies have suggested that selection performs poorly in pericentromeric regions because of reduced recombination frequency (RF). Thus, manipulating RF and meiotic crossover location is an appealing issue to advance crop breeding. In our study, we are using genomic analysis to characterize intragenomic diversity of tropical elite soybean lines in terms of heterozygosity, RF, chromatin structure and accessibility. Our results to date include the generation of draft genome assemblies for two soybean advanced lines using PACBIO long-read sequencing, Chromium Linked-Reads sequencing and an approach rooted in polymer physics that determines the most likely genome structure using chromosomal contact data. In-depth genome-wide characterization is ongoing using ATAC-Seq and Parent-Progeny resequencing. We also present our efforts to apply DNA-free editing tools in implementing genetic alterations that are expected to engineer desirable traits in soybeans by modifying meiotic specific expression. These changes are expected to increase genetic and epigenetic variations with favorable outcomes in seed oil and protein pathway regulation. Financial support: Embrapa-Monsanto Fund/Project:03.17.03.002.00.00 MenosABSTRACT: Directional selection during crop domestication and advanced breeding has resulted in significant changes in plant genomes. The extent of intraspecific variation in a crop documents domestication and highlights variants underlying complex traits. Studies demonstrate that most naturally occurring variants in crops are low frequency, and only a small fraction of those might have important functions to trait variation. To understand the inheritance of quantitative traits, diversity panels have become available by crossing inbred lines to produce genomic maps that relate phenotypic variation to recombination and ultimately to genome structure. Using these resources, genomics studies have suggested that selection performs poorly in pericentromeric regions because of reduced recombination frequency (RF). Thus, manipulating RF and meiotic crossover location is an appealing issue to advance crop breeding. In our study, we are using genomic analysis to characterize intragenomic diversity of tropical elite soybean lines in terms of heterozygosity, RF, chromatin structure and accessibility. Our results to date include the generation of draft genome assemblies for two soybean advanced lines using PACBIO long-read sequencing, Chromium Linked-Reads sequencing and an approach rooted in polymer physics that determines the most likely genome structure using chromosomal contact data. In-depth genome-wide characterization is ongoing using ATAC-Seq and Parent-Progeny resequencing. We ... Mostrar Tudo |
Palavras-Chave: |
Análise genômica; Seqüenciamento de longa duração. |
Thesagro: |
Genoma; Melhoramento Genético Vegetal; Soja. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187384/1/Coupling-in-depth-genome-annotations-with-genome-editing-technology-for-harnessing-genomic-variation-to-promote-precision-breeding-in-tropical-soybean-APAGAR.pdf
|
Marc: |
LEADER 02810nam a2200241 a 4500 001 2100401 005 2018-11-29 008 2018 bl uuuu u00u1 u #d 100 1 $aSILVA JUNIOR, O. B. da 245 $aCoupling in-depth genome annotations with genome editing technology for harnessing genomic variation to promote precision breeding in tropical soybean.$h[electronic resource] 260 $aIn: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec$c2018 520 $aABSTRACT: Directional selection during crop domestication and advanced breeding has resulted in significant changes in plant genomes. The extent of intraspecific variation in a crop documents domestication and highlights variants underlying complex traits. Studies demonstrate that most naturally occurring variants in crops are low frequency, and only a small fraction of those might have important functions to trait variation. To understand the inheritance of quantitative traits, diversity panels have become available by crossing inbred lines to produce genomic maps that relate phenotypic variation to recombination and ultimately to genome structure. Using these resources, genomics studies have suggested that selection performs poorly in pericentromeric regions because of reduced recombination frequency (RF). Thus, manipulating RF and meiotic crossover location is an appealing issue to advance crop breeding. In our study, we are using genomic analysis to characterize intragenomic diversity of tropical elite soybean lines in terms of heterozygosity, RF, chromatin structure and accessibility. Our results to date include the generation of draft genome assemblies for two soybean advanced lines using PACBIO long-read sequencing, Chromium Linked-Reads sequencing and an approach rooted in polymer physics that determines the most likely genome structure using chromosomal contact data. In-depth genome-wide characterization is ongoing using ATAC-Seq and Parent-Progeny resequencing. We also present our efforts to apply DNA-free editing tools in implementing genetic alterations that are expected to engineer desirable traits in soybeans by modifying meiotic specific expression. These changes are expected to increase genetic and epigenetic variations with favorable outcomes in seed oil and protein pathway regulation. Financial support: Embrapa-Monsanto Fund/Project:03.17.03.002.00.00 650 $aGenoma 650 $aMelhoramento Genético Vegetal 650 $aSoja 653 $aAnálise genômica 653 $aSeqüenciamento de longa duração 700 1 $aPESSOA FILHO, M. A. C. de P. 700 1 $aSA, M. E. L. 700 1 $aDEAL, R. 700 1 $aRAGOUSSIS, I. 700 1 $aGRATTAPAGLIA, D. 700 1 $aRECH FILHO, E. L.
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