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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
20/12/2019 |
Data da última atualização: |
20/12/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
IARK, D.; BUZZO, A. J. dos R.; GARCIA, J. A. A.; CORREA, V. G.; HELM, C. V.; CORRÊA, R. C. G.; PERALTA, R. A.; MOREIRA, R. de F. P. M.; BRACHT, A.; PERALTA, R. M. |
Afiliação: |
Daiane Iark, UEM; Ana Júlia dos Reis Buzzo, UEM; Jéssica Amanda Andrade Garcia, UEM; Vanesa Gesser Correa, UEM; CRISTIANE VIEIRA HELM, CNPF; Rúbia Carvalho Gomes Corrêa, UEM; Rosely A. Peralta, UFSC; Regina de Fátima Peralta Muniz Moreira, UFSC; Adelar Bracht, UEM; Rosane Marina Peralta, UEM. |
Título: |
Enzymatic degradation and detoxification of azo dye Congo red by a new laccase from Oudemansiella canarii. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Bioresource Technology, 289, article 121655, Oct. 2019. 7 p. |
DOI: |
10.1016/j.biortech.2019.121655 |
Idioma: |
Inglês |
Conteúdo: |
A single laccase with molecular weight of 41 kDa was produced by the white-rot fungus Oudemansiella canarii cultured on solid state fermentation using a mixture of sugarcane bagasse-wheat bran as substrate. The enzyme (5 U) was able to decolourize 80% of 50 mg/L Congo red within 24 h at 30 °C and pH 5.5. The relationship between the decolorization rate and dye concentration obeyed Michaelis?Menten kinetics, with KM and Vmax values of 46.180 ± 6.245 µM and 1.840 ± 0.101 µmol/min, respectively. Fourier transform infrared spectroscopy (FTIR) and mass spectrometry allowed to conclude that the laccase acts not only on the dye chromophore group, but also that it cleaves different covalent bonds, causing an effective fragmentation of the molecule. The action of the laccase caused a significant reduction in toxicity, as indicated by the Microtox test. In conclusion, O. canarii laccase could be useful in future biological strategies aiming at degrading azo dyes. |
Palavras-Chave: |
Biorremediação; Dye degradation; Lacase. |
Thesaurus Nal: |
Azo dyes; Laccase; White-rot fungi. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 01891naa a2200313 a 4500 001 2117496 005 2019-12-20 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1016/j.biortech.2019.121655$2DOI 100 1 $aIARK, D. 245 $aEnzymatic degradation and detoxification of azo dye Congo red by a new laccase from Oudemansiella canarii.$h[electronic resource] 260 $c2019 520 $aA single laccase with molecular weight of 41 kDa was produced by the white-rot fungus Oudemansiella canarii cultured on solid state fermentation using a mixture of sugarcane bagasse-wheat bran as substrate. The enzyme (5 U) was able to decolourize 80% of 50 mg/L Congo red within 24 h at 30 °C and pH 5.5. The relationship between the decolorization rate and dye concentration obeyed Michaelis?Menten kinetics, with KM and Vmax values of 46.180 ± 6.245 µM and 1.840 ± 0.101 µmol/min, respectively. Fourier transform infrared spectroscopy (FTIR) and mass spectrometry allowed to conclude that the laccase acts not only on the dye chromophore group, but also that it cleaves different covalent bonds, causing an effective fragmentation of the molecule. The action of the laccase caused a significant reduction in toxicity, as indicated by the Microtox test. In conclusion, O. canarii laccase could be useful in future biological strategies aiming at degrading azo dyes. 650 $aAzo dyes 650 $aLaccase 650 $aWhite-rot fungi 653 $aBiorremediação 653 $aDye degradation 653 $aLacase 700 1 $aBUZZO, A. J. dos R. 700 1 $aGARCIA, J. A. A. 700 1 $aCORREA, V. G. 700 1 $aHELM, C. V. 700 1 $aCORRÊA, R. C. G. 700 1 $aPERALTA, R. A. 700 1 $aMOREIRA, R. de F. P. M. 700 1 $aBRACHT, A. 700 1 $aPERALTA, R. M. 773 $tBioresource Technology, 289, article 121655, Oct. 2019. 7 p.
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Embrapa Florestas (CNPF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
28/04/2020 |
Data da última atualização: |
28/04/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
RAVISANKAR, S.; QUEIROZ, V. A. V.; AWIKA, J. M. |
Afiliação: |
Shreeya Ravisankar, Texas A&M University; VALERIA APARECIDA VIEIRA QUEIROZ, CNPMS; Joseph M. Awika, Texas A&M University. |
Título: |
Rye flavonoids: structural profile of the flavones in diverse varieties and effect of fermentation and heat on their structure and antioxidant properties. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Food Chemistry, v. 324, article 126871, 2020. |
DOI: |
https://doi.org/10.1016/j.foodchem.2020.126871 |
Idioma: |
Inglês |
Conteúdo: |
Flavones are important bioactive flavonoids in cereal grains, but are poorly characterized. This study investigated rye flavone profile as influenced by phenotype (grain color), sourdough fermentation, and subsequent heat processing. Twelve rye varieties belonging to 4 phenotypes were characterized using UPLC-tandem quadrupole MS before and after fermentation and baking. Antioxidant properties were also assessed. Rye flavones (range 57?137 μg/g) were dominated by O-glycosides (50?68%), present as derivatives of tricin (exclusively O-glycosides), chrysoeriol (O-/C-glycosides), and apigenin (exclusively C-glycosides). Phenotype did not influence flavone content. Fermentation partially hydrolyzed O-glycosides to their aglycones, but did not affect C-glycosides. Extractable phenols and antioxidants increased 1.9?3.6X after 96-h fermentation; baking further increased these components by 36?96% in fermented samples, likely via enhanced cell wall disruption. The high proportion of flavone-O-glycosides in rye is of interest due to their known higher bioavailability relative to typical cereal grain C-glycosides. |
Palavras-Chave: |
Atividade antioxidante. |
Thesagro: |
Centeio; Fermentação; Flavonóide. |
Categoria do assunto: |
Q Alimentos e Nutrição Humana |
Marc: |
LEADER 01824naa a2200205 a 4500 001 2121871 005 2020-04-28 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.foodchem.2020.126871$2DOI 100 1 $aRAVISANKAR, S. 245 $aRye flavonoids$bstructural profile of the flavones in diverse varieties and effect of fermentation and heat on their structure and antioxidant properties.$h[electronic resource] 260 $c2020 520 $aFlavones are important bioactive flavonoids in cereal grains, but are poorly characterized. This study investigated rye flavone profile as influenced by phenotype (grain color), sourdough fermentation, and subsequent heat processing. Twelve rye varieties belonging to 4 phenotypes were characterized using UPLC-tandem quadrupole MS before and after fermentation and baking. Antioxidant properties were also assessed. Rye flavones (range 57?137 μg/g) were dominated by O-glycosides (50?68%), present as derivatives of tricin (exclusively O-glycosides), chrysoeriol (O-/C-glycosides), and apigenin (exclusively C-glycosides). Phenotype did not influence flavone content. Fermentation partially hydrolyzed O-glycosides to their aglycones, but did not affect C-glycosides. Extractable phenols and antioxidants increased 1.9?3.6X after 96-h fermentation; baking further increased these components by 36?96% in fermented samples, likely via enhanced cell wall disruption. The high proportion of flavone-O-glycosides in rye is of interest due to their known higher bioavailability relative to typical cereal grain C-glycosides. 650 $aCenteio 650 $aFermentação 650 $aFlavonóide 653 $aAtividade antioxidante 700 1 $aQUEIROZ, V. A. V. 700 1 $aAWIKA, J. M. 773 $tFood Chemistry$gv. 324, article 126871, 2020.
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