|
|
Registros recuperados : 4 | |
1. | | TIROLI, A. O.; TASIC, L.; OLIVEIRA, C. L. P.; BLOCH JUNIOR, C.; TORRIANI, I.; FARAH, C. S.; RAMOS, C. H. I. Mapping contacts between regulatory domains of skeletal muscle TnC and Tnl by analyses of a single-chain chimeras. The FEBS Journal, v. 272, n. 2, p. 779-790, 2005. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
2. | | VILLELA, A. D.; DUCATI, R. G.; ROSADO, L. A.; BLOCH JUNIOR, C.; PRATES, M. V.; GONÇALVES, D. C.; RAMOS, C. H. I.; BASSO, L. A.; SANTOS, D. S. Biochemical characterization of uracil phosphoribosyltransferase from Mycobacterium tuberculosis. Plos One, v. 8, n. 2, e56445, 2013. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
3. | | MARES-GUIA, T. R.; MAIGRET, B.; MARTINS, N. F.; MAIA, A. L. T.; VILELA, L.; RAMOS, C. H. I.; JULIANO NETO, L.; JULIANO, M. A.; MARES-GUIA, M. L. dos; SANTORO, M. M. Molecular dynamics and circular dichroism studies of human and rat C-peptides. Journal of Molecular Graphics and Modelling, v. 25, n. 4, p. 532-542, Dec. 2006. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
4. | | SILVEIRA, C. H. da; PIRES, D. E. V.; MINARDI, R.; RIBEIRO, C.; VELOSO, C. J. M.; LOPES, J. C. D.; MEIRA JÚNIOR, W.; NESHICH, G.; RAMOS, C. H. I.; HABESCH, R.; SANTORO, M. M. Protein cutoff scanning: a comparative analysis of cutoff dependent and cutoff free methods for prospecting contacts in proteins. Proteins, n. 74, p. 727-743, 2009. Biblioteca(s): Embrapa Agricultura Digital. |
| |
Registros recuperados : 4 | |
|
|
Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
15/04/2010 |
Data da última atualização: |
28/09/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SILVEIRA, C. H. da; PIRES, D. E. V.; MINARDI, R.; RIBEIRO, C.; VELOSO, C. J. M.; LOPES, J. C. D.; MEIRA JÚNIOR, W.; NESHICH, G.; RAMOS, C. H. I.; HABESCH, R.; SANTORO, M. M. |
Afiliação: |
CARLOS H. DA SILVEIRA, UFMG; DOUGLAS E. V. PIRES, UFMG; RAQUEL MINARDI, UFMG; CRISTINA RIBEIRO, UFMG; CAIO J. M. VELOSO, PUC-MG; JULIO C. D. LOPES, UFMG; WAGNER MEIRA JÚNIOR, UFMG; GORAN NESHICH, CNPTIA; CARLOS H. I. RAMOS, UNICAMP; RAUL HABESCH, UFMG; MARCELO M. SANTORO, UFMG. |
Título: |
Protein cutoff scanning: a comparative analysis of cutoff dependent and cutoff free methods for prospecting contacts in proteins. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Proteins, n. 74, p. 727-743, 2009. |
Idioma: |
Inglês |
Conteúdo: |
In this study, we carried out a comparative analysis between two classical methodologies to prospect residue contacts in proteins: the traditional cutoff dependent (CD) approach and cutoff free Delaunay tessellation (DT). In addition, two alternative coarse-grained forms to represent residues were tested: using alpha carbon (CA) and side chain geometric center (GC). A database was built, comprising three top classes: all alpha, all beta, and alpha/beta. We found that the cutoff value? at about 7.0 A emerges as an important distance parameter.? Up to 7.0 A, CD and DT properties are unified, which implies that at this distance all contacts are complete and legitimate (not occluded). We also have shown that DT has an intrinsic missing edges problem when mapping the first layer of neighbors. In proteins, it may produce systematic errors affecting mainly the contact network in beta chains with CA. The almost-Delaunay (AD) approach has been proposed to solve this DT problem. We found that even AD may not be an advantageous solution. As a consequence, in the strict range up ? to 7.0 A, the CD approach revealed to be a simpler, more complete, and reliable technique than DT or AD. Finally, we have shown that coarse-grained residue representations may introduce bias in the analysis of neighbors in cutoffs up to ? 6.8 A, with CA favoring alpha proteins and GC favoring beta proteins. This provides an additional argument pointing to ? the value of 7.0 A as an important lower bound cutoff to be used in contact analysis of proteins. MenosIn this study, we carried out a comparative analysis between two classical methodologies to prospect residue contacts in proteins: the traditional cutoff dependent (CD) approach and cutoff free Delaunay tessellation (DT). In addition, two alternative coarse-grained forms to represent residues were tested: using alpha carbon (CA) and side chain geometric center (GC). A database was built, comprising three top classes: all alpha, all beta, and alpha/beta. We found that the cutoff value? at about 7.0 A emerges as an important distance parameter.? Up to 7.0 A, CD and DT properties are unified, which implies that at this distance all contacts are complete and legitimate (not occluded). We also have shown that DT has an intrinsic missing edges problem when mapping the first layer of neighbors. In proteins, it may produce systematic errors affecting mainly the contact network in beta chains with CA. The almost-Delaunay (AD) approach has been proposed to solve this DT problem. We found that even AD may not be an advantageous solution. As a consequence, in the strict range up ? to 7.0 A, the CD approach revealed to be a simpler, more complete, and reliable technique than DT or AD. Finally, we have shown that coarse-grained residue representations may introduce bias in the analysis of neighbors in cutoffs up to ? 6.8 A, with CA favoring alpha proteins and GC favoring beta proteins. This provides an additional argument pointing to ? the value of 7.0 A as an important lower bound cutoff... Mostrar Tudo |
Thesagro: |
Proteína. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/147896/1/Proteins-Structure-Function-and-Bioinformatics.pdf
|
Marc: |
LEADER 02290naa a2200253 a 4500 001 1664411 005 2016-09-28 008 2009 bl uuuu u00u1 u #d 100 1 $aSILVEIRA, C. H. da 245 $aProtein cutoff scanning$ba comparative analysis of cutoff dependent and cutoff free methods for prospecting contacts in proteins.$h[electronic resource] 260 $c2009 520 $aIn this study, we carried out a comparative analysis between two classical methodologies to prospect residue contacts in proteins: the traditional cutoff dependent (CD) approach and cutoff free Delaunay tessellation (DT). In addition, two alternative coarse-grained forms to represent residues were tested: using alpha carbon (CA) and side chain geometric center (GC). A database was built, comprising three top classes: all alpha, all beta, and alpha/beta. We found that the cutoff value? at about 7.0 A emerges as an important distance parameter.? Up to 7.0 A, CD and DT properties are unified, which implies that at this distance all contacts are complete and legitimate (not occluded). We also have shown that DT has an intrinsic missing edges problem when mapping the first layer of neighbors. In proteins, it may produce systematic errors affecting mainly the contact network in beta chains with CA. The almost-Delaunay (AD) approach has been proposed to solve this DT problem. We found that even AD may not be an advantageous solution. As a consequence, in the strict range up ? to 7.0 A, the CD approach revealed to be a simpler, more complete, and reliable technique than DT or AD. Finally, we have shown that coarse-grained residue representations may introduce bias in the analysis of neighbors in cutoffs up to ? 6.8 A, with CA favoring alpha proteins and GC favoring beta proteins. This provides an additional argument pointing to ? the value of 7.0 A as an important lower bound cutoff to be used in contact analysis of proteins. 650 $aProteína 700 1 $aPIRES, D. E. V. 700 1 $aMINARDI, R. 700 1 $aRIBEIRO, C. 700 1 $aVELOSO, C. J. M. 700 1 $aLOPES, J. C. D. 700 1 $aMEIRA JÚNIOR, W. 700 1 $aNESHICH, G. 700 1 $aRAMOS, C. H. I. 700 1 $aHABESCH, R. 700 1 $aSANTORO, M. M. 773 $tProteins$gn. 74, p. 727-743, 2009.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|