| |
|
|
| Registros recuperados : 23 | |
| 6. |  | CANEL, N. G.; BEVACQUA, R. J.; HIRIART, M. I.; RABELO, N. C.; CAMARGO, L. S. de A.; SALAMONE, D. F. Approaches to improve intracytoplasmic sperm injection mediated transgenesis and maximize the use of sex-sorted sperm in bovine In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 41., 2015, Versailles. Reproductive performance: at the crossroads of genetics and the environment: abstracts. Versailles: IETS, 2015. p. 248.| Biblioteca(s): Embrapa Gado de Leite. |
|    |
| 7. | | DIAS, T. A. B.; SCHIAVINI, F.; RABELO, N.; SILVA, S.; ARATANHA, V.; KRAHÔ, G.; KRAHÔ, M.; KRAHÔ, F. Estratégias de conservação da agrobiodiversidade indígena: feiras Krahô de sementes tradicionais. In: SIMPOSIO INTERNACIONAL DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, 8., 2011, Quito. [Proceedings...] Quito: INIAP, 2011.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 8. | | RABELO, N. C.; GHETTI, A. M.; CAMARGO, L. S. de A.; VERNEQUE, R. da S.; VIANA, J. H. M. Uso do cultivo overnight na avaliação do potencial de desenvolvimento embrionário e como alternativa na logística da transferência a fresco de embriões F1 Holandês/Gir. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 5., 2010, Juiz de Fora, MG. Anais... Juiz de Fora: Embrapa Gado de Leite, 2010. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 141.). p. 33-36.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 9. | | CANEL, N. G.; BEVACQUA, R. J.; HIRIART, M. I.; RABELO, N. C.; CAMARGO, L. S. de A.; ROMANATO, M.; CALVO, L. P.; SALAMONE, D. F. Sperm pretreatment with heparin and L-glutathione, sex-sorting, and double cryopreservation to improve intracytoplasmic sperm injection in bovine. Theriogenology, v. 93, p. 62-70, 2017.| Biblioteca(s): Embrapa Gado de Leite. |
|  |
| 10. | | SOUZA, E. D.; RABELO, N. C.; ARAÚJO, T. D.; ASSUNÇÃO, C. M.; QUINTAO, C. C. R.; VIANA, J. H. M.; LOURO, I. D.; CAMARGO, L. S. de A. Inhibition of HSP90 aggravates the effects of heat shock on developmental competence of bovine oocytes In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 41., 2015, Versailles. Reproductive performance: at the crossroads of genetics and the environment: abstracts. Versailles: IETS, 2015. p. 235.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 11. | | SOUZA, E. P. D. De; RABELO, N. C.; DIAS, R. B S.; SOUZA, G. T. De; LOURO, I. D.; CAMARGO, L. S. de A. Inibição da HSP90 durante maturação interfere na abundância relativa de transcritos específicos oócito bovino. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 12. | | QUINTAO, C. C. R.; PEREIRA, M. M.; RABELO, N. C.; ALONSO, J. R. T.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. de A. Efeito da passagem celular na produção de embriões da raça gir pela técnica de transferência nuclear. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, 25., 2011, Cumbuco. Resumos... p. 435| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 13. | | RABELO, N. C.; QUINTAO, C. C. R.; BRITO, S. G.; RAPOSO, N. R. B.; SOUZA, E. D.; VIANA, J. H. M.; CAMARGO, L. S. de A. Reversibility of cell cycle inhibition induced by extracts of azadirachta indica in bovine fibroblasts. Animal Reproduction, v. 9, n. 3, p. 652, 2012. Edição dos abstracts do 26º Animal Meeting of the Brazilian Embryo Technology Society, Foz do Iguaçu, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 14. | | PEREIRA, M. M.; BRANDAO, H. de M.; RAPOSO, N. R. B.; QUINTAO, C. C. R.; SILVA, S. R. DA; RABELO, N. C.; CAMARGO, L. S. de A.; LADEIRA, L. O. Effect of carboxylate multi-walled carbon nanotubes on the cell viability in bovine dermal fibroblast. In: SEMINÁRIO INTERNACIONAL DE NANOTECNOLOGIA, SOCIEDADE E MEIO AMBIENTE, 7., 2010, Rio de Janeiro. Resumos... Rio de Janeiro: FIOCRUZ, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 15. | | QUINTAO, C. C. R.; RABELO, N. C.; PEREIRA, M. M.; GIACOMINI, S. B.; MOREIRA, A. P.; RAPOSO, N. R. B.; CARVALHO, B. C. de; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. de A. Cell cycle synchronization of bovine fibroblasts by Azadirachta indica extracts. In: Annual Conference of the Internacional Embryo Transfes Society, 2012, Phoenix. Procidings...| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 16. | | SOUZA, E. D.; PAULA, F. B. E.; RABELO, N. C.; ASSUNÇÃO, C. M.; QUINTAO, C. C. R.; ARASHIRO, E. K. N.; VIANA, J. H. M.; LOURO, I. D.; CAMARGO, L. S. de A. Apoptpsis in embryos exposed to the HSP90 inhibitor during in vitro maturation of bovine oocytes. Animal Reproduction, v. 11, n. 3, p. 412, 2014. Edição dos proceedings do 28º Annual Meeting of the Brazilian Embryo Technology Society, 2014, Natal.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 17. | | PEREIRA, M. M.; WOHLRES-VIANA, S.; SALES, J. N. S.; CAMARGO, A. R.; QUINTAO, C. C. R.; RABELO, N. C.; CARVALHO, B. C. de; VIANA, J. H. M.; CAMARGO, L. S. de A.; GUIMARAES, M. F. M.; IGUMA, L. T. Differential gene expression od in vitro-matured bovine oocytes qith od without a polar body. In: ANNUAL CONFERENCE OF THE INTERNACIONAL EMBRYO TRANSFES SOCIETY, 2012, Phoenix. Proceedings... [S.l.:s.n.], 2012.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 18. | | RABELO, N. C.; QUINTAO, C. C. R.; MOREIRA, A. P.; BRITO, S. G.; PEREIRA, M. M.; NUNES, J. D.; AZEVEDO, A. L. S.; RAPOSO, N. B. R.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. de A. Efeito do extrato etanólico de Azadirachta indica na sincronização do ciclo celular de fibroblastos bovinos. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, 25., 2011, Cumbuco. Resumos... p. 332| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 19. | | BRITO, S. G.; QUINTAO, C. C. R.; SOUZA, E. D. DE; RABELO, N. C.; PEREIRA, M. M.; IGUMA, L. T.; CARVALHO, B. C. de; VIANA, J. H. M.; CAMARGO, L. S. de A. Inibição de histona deacetilase por tricostatina A em embriões bovinos produzidos por transferência nuclear com células somáticas. In: Workshop de Iniciação Científica da Embrapa Gado de Leite, 8., 2011, Juiz de Fora. Anais... Documentos, 151.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 20. | | RABELO, N. C.; QUINTAO, C. C. R.; RETTORE, J. V. P.; BOLPATO, M. C. L.; RAPOSO, N. B. R.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. de A. Evaluation of cell viability and gene expression of stress and apoptosis in bovine fibroblasts exposed to ethanol extract of Azadirachta indica. Animal Reproduction, v. 10, n. 3, p. 610, Jul./Sept. 2013. Suplemento. Edição dos abstracts do 27º Annual Meeting of the Brazilian Embryo Technology Society, 2013, Praia do Forte, BA.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| Registros recuperados : 23 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
17/01/2018 |
|
Data da última atualização: |
27/01/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
CANEL, N. G.; BEVACQUA, R. J.; HIRIART, M. I.; RABELO, N. C.; CAMARGO, L. S. de A.; ROMANATO, M.; CALVO, L. P.; SALAMONE, D. F. |
|
Afiliação: |
Natália Gabriela Canel, Universidade de Buenos Aires; Romina Jimena Bevacqua, Universidade de Buenos Aires; María Inés Hiriart, Universidade de Buenos Aires; Natana Chaves Rabelo, UFJF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Marina Romanato, CONICET; Lucrecia Pineiro de Calvo, CONICET; Daniel Felipe Salamone, Universidade de Buenos Aires. |
|
Título: |
Sperm pretreatment with heparin and L-glutathione, sex-sorting, and double cryopreservation to improve intracytoplasmic sperm injection in bovine. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Theriogenology, v. 93, p. 62-70, 2017. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract In bovine, intracytoplasmic sperm injection (ICSI) remains inefficient partially due to low levels of sperm decondensation. The aim of this study was to determine whether the injection of normal size sperm pretreated with heparin (Hep) and l-glutathione (GSH), the use of sex-sorted sperm, the double round of sperm freezing/thawing (re frozen), or the combination of these approaches can improve sperm decondensation and embryo development after ICSI in cattle. Cleavage and blastocyst rates were evaluated on days 2 and 7 post ICSI. Quality of ICSI blastocysts was analyzed by the relative expression of four genes by qPCR and the DNA fragmentation index by TUNEL assay. For all assays, semen samples were co-incubated with pCX-EGFP 50 ng/μl before ICSI. GFP expression, which can be detected by fluorescence microscopy, was used as a tool to estimate the success of sperm decondensation after ICSI. The use of normal size sperm pretreated with 80 μM Hep-15 mM GSH for 20 h (Hep-GSH) increased cleavage, blastocyst and EGFP + blastocysts rates (60.8, 19.4 and 61.9%) compared to control ICSI (35, 4.9 and 20%) (p < 0.05). Moreover, HMGN1, GLUT5, AQP3 and POU5F1 transcription levels did not differ between ICSI Hep-GSH and IVF embryos. The use of sex-sorted sperm (X, Y) improved cleavage rates and EGFP expression at day 4 (83 and 30.2% for ICSI Y and 83.2 and 31.7% for ICSI X) compared to non-sorted group (50.9 and 15.1%), not showing differences at the blastocyst stage. Finally, sex sorting (X) was combined with Hep-GSH and/or re frozen treatments. The use of Hep-GSH diminished cleavage rates from ICSI X re frozen group (80.4% vs. 94.2%) and blastocyst development from ICSI X group (3.3% vs. 10%), compared with their controls (p < 0.05). While Hep-GSH pretreatment induced lower transgene expression at day 4, no differences were found at the blastocyst stage between ICSI groups (from 58.3 to 80%). TUNEL assay showed higher DNA fragmentation indexes for all ICSI treatments (p < 0.05), except for ICSI X Hep-GSH, which did not differ from IVF X control. In conclusion, the use of normal size sperm pretreated with Hep-GSH, combined or not with sex-sorting by flow cytometry could improve ICSI outcomes in cattle. MenosAbstract In bovine, intracytoplasmic sperm injection (ICSI) remains inefficient partially due to low levels of sperm decondensation. The aim of this study was to determine whether the injection of normal size sperm pretreated with heparin (Hep) and l-glutathione (GSH), the use of sex-sorted sperm, the double round of sperm freezing/thawing (re frozen), or the combination of these approaches can improve sperm decondensation and embryo development after ICSI in cattle. Cleavage and blastocyst rates were evaluated on days 2 and 7 post ICSI. Quality of ICSI blastocysts was analyzed by the relative expression of four genes by qPCR and the DNA fragmentation index by TUNEL assay. For all assays, semen samples were co-incubated with pCX-EGFP 50 ng/μl before ICSI. GFP expression, which can be detected by fluorescence microscopy, was used as a tool to estimate the success of sperm decondensation after ICSI. The use of normal size sperm pretreated with 80 μM Hep-15 mM GSH for 20 h (Hep-GSH) increased cleavage, blastocyst and EGFP + blastocysts rates (60.8, 19.4 and 61.9%) compared to control ICSI (35, 4.9 and 20%) (p < 0.05). Moreover, HMGN1, GLUT5, AQP3 and POU5F1 transcription levels did not differ between ICSI Hep-GSH and IVF embryos. The use of sex-sorted sperm (X, Y) improved cleavage rates and EGFP expression at day 4 (83 and 30.2% for ICSI Y and 83.2 and 31.7% for ICSI X) compared to non-sorted group (50.9 and 15.1%), not showing differences at the blastocyst stage. Fi... Mostrar Tudo |
|
Palavras-Chave: |
ICSI; Pcx-EGFP; Sex-sorted sperm; Sperm pretreatment. |
|
Thesaurus NAL: |
cattle. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 03038naa a2200265 a 4500
001 2085654
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
100 1 $aCANEL, N. G.
245 $aSperm pretreatment with heparin and L-glutathione, sex-sorting, and double cryopreservation to improve intracytoplasmic sperm injection in bovine.$h[electronic resource]
260 $c2017
520 $aAbstract In bovine, intracytoplasmic sperm injection (ICSI) remains inefficient partially due to low levels of sperm decondensation. The aim of this study was to determine whether the injection of normal size sperm pretreated with heparin (Hep) and l-glutathione (GSH), the use of sex-sorted sperm, the double round of sperm freezing/thawing (re frozen), or the combination of these approaches can improve sperm decondensation and embryo development after ICSI in cattle. Cleavage and blastocyst rates were evaluated on days 2 and 7 post ICSI. Quality of ICSI blastocysts was analyzed by the relative expression of four genes by qPCR and the DNA fragmentation index by TUNEL assay. For all assays, semen samples were co-incubated with pCX-EGFP 50 ng/μl before ICSI. GFP expression, which can be detected by fluorescence microscopy, was used as a tool to estimate the success of sperm decondensation after ICSI. The use of normal size sperm pretreated with 80 μM Hep-15 mM GSH for 20 h (Hep-GSH) increased cleavage, blastocyst and EGFP + blastocysts rates (60.8, 19.4 and 61.9%) compared to control ICSI (35, 4.9 and 20%) (p < 0.05). Moreover, HMGN1, GLUT5, AQP3 and POU5F1 transcription levels did not differ between ICSI Hep-GSH and IVF embryos. The use of sex-sorted sperm (X, Y) improved cleavage rates and EGFP expression at day 4 (83 and 30.2% for ICSI Y and 83.2 and 31.7% for ICSI X) compared to non-sorted group (50.9 and 15.1%), not showing differences at the blastocyst stage. Finally, sex sorting (X) was combined with Hep-GSH and/or re frozen treatments. The use of Hep-GSH diminished cleavage rates from ICSI X re frozen group (80.4% vs. 94.2%) and blastocyst development from ICSI X group (3.3% vs. 10%), compared with their controls (p < 0.05). While Hep-GSH pretreatment induced lower transgene expression at day 4, no differences were found at the blastocyst stage between ICSI groups (from 58.3 to 80%). TUNEL assay showed higher DNA fragmentation indexes for all ICSI treatments (p < 0.05), except for ICSI X Hep-GSH, which did not differ from IVF X control. In conclusion, the use of normal size sperm pretreated with Hep-GSH, combined or not with sex-sorting by flow cytometry could improve ICSI outcomes in cattle.
650 $acattle
653 $aICSI
653 $aPcx-EGFP
653 $aSex-sorted sperm
653 $aSperm pretreatment
700 1 $aBEVACQUA, R. J.
700 1 $aHIRIART, M. I.
700 1 $aRABELO, N. C.
700 1 $aCAMARGO, L. S. de A.
700 1 $aROMANATO, M.
700 1 $aCALVO, L. P.
700 1 $aSALAMONE, D. F
773 $tTheriogenology$gv. 93, p. 62-70, 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
