Data/hora: 25/06/2024 05:37:01

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 31/05/2007
Data da última atualização: | 08/07/2019
Autoria: | ESTRADA-NAVARRETE, G.; ALVARADO-AFFANTRANGER, X.; OLIVARES, J.-E.; DÍAZ-CAMINO, C.; SANTANA, O.; MURILLO, E.; GUILLÉN, G.; SÁNCHEZ-GUEVARA, N.; ACOSTA, J.; QUINTO, C.; LI, D.; GRESSHOFF, P. M.; SÁNCHEZ, F.
Título: | Agrobacterium rhizogenes transformation of the Phaseolus spp.: a tool for functional genomics.
Ano de publicação: | 2006
Fonte/Imprenta: | Molecular Plant-Microbe Interactions, St. Paul, v. 19, n. 12, p. 1385-1393, 2006.
Idioma: | Inglês
Conteúdo: | A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes  was developed for Phaseolus vulgaris L. wild accessions, land races, and culti-vars and for three other species belonging to the genus Phaseolus: P. coccineus, P. lunatus, and P. acutifolius. In-duced hairy roots are robust and grow quickly. The trans-formation frequency is between 75 and 90% based on the 35.S promoter-driven green fluorescent protein and f)-glu-curonidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal de-terminate nodules that fix nitrogen as etliciently as inocu-lated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiol-ogy, root metabolism, and root-microbe interactions.
Thesaurus Nal: | Agrobacterium.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (34163-1) - UPC - SP - -- -  -  - 0023205 - DIS