|
|
Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
27/11/2019 |
Data da última atualização: |
27/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MENDES, L. W.; CHAVES, M. G. de; FONSECA, M. de C.; MENDES, R.; RAAIJMAKERS, J. M.; TSAI, S. M. |
Afiliação: |
LUCAS WILLIAM MENDES, CENA-USP; MIRIAM GONCALVES DE CHAVES, CENA-USP; MARILEY DE CASSIA FONSECA, CENA-USP; RODRIGO MENDES, CNPMA; JOOS M RAAIJMAKERS, Netherlands Institute of Ecology; SIU MUI TSAI, CENA-USP. |
Título: |
Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Frontiers in Microbiology, v. 10, 2019. Article 2252. |
DOI: |
https://doi.org/10.3389/fmicb.2019.02252 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The taxonomically diverse rhizosphere microbiome contributes to plant nutrition, growth and health, including protection against soil-borne pathogens. We previously showed that breeding for Fusarium-resistance in common bean changed the rhizosphere microbiome composition and functioning. Here, we assessed the impact of Fusarium-resistance breeding in common bean on microbiome physiology. Combined with metatranscriptome data, community-level physiological profiling by Biolog EcoPlate analyses revealed that the rhizosphere microbiome of the Fusarium-resistant accession was distinctly different from that of the Fusarium-susceptible accession, with higher consumption of amino acids and amines, higher metabolism of xylanase and sialidase, and higher expression of genes associated with nitrogen, phosphorus and iron metabolism. The resistome analysis indicates higher expression of soxR, which is involved in protecting bacteria against oxidative stress induced by a pathogen invasion. These results further support our hypothesis that breeding for resistance has unintentionally shaped the assembly and activity of the rhizobacterial community toward a higher abundance of specific rhizosphere competent bacterial taxa that can provide complementary protection against fungal root infections. |
Palavras-Chave: |
Biolog EcoPlate; Metatranscriptome; Nutrientmetabolism; Plant-microbe interactions; Resistome. |
Thesagro: |
Feijão; Microbiologia do Solo; População Microbiana; Resistência. |
Thesaurus Nal: |
Beans; Carbohydrate metabolism; Disease resistance; Microbiome; Soil-plant interactions. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/205686/1/Mendes-Resistance-Breeding-2019.pdf
|
Marc: |
LEADER 02378naa a2200361 a 4500 001 2115347 005 2019-11-27 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3389/fmicb.2019.02252$2DOI 100 1 $aMENDES, L. W. 245 $aResistance breeding of common bean shapes the physiology of the rhizosphere microbiome.$h[electronic resource] 260 $c2019 520 $aAbstract: The taxonomically diverse rhizosphere microbiome contributes to plant nutrition, growth and health, including protection against soil-borne pathogens. We previously showed that breeding for Fusarium-resistance in common bean changed the rhizosphere microbiome composition and functioning. Here, we assessed the impact of Fusarium-resistance breeding in common bean on microbiome physiology. Combined with metatranscriptome data, community-level physiological profiling by Biolog EcoPlate analyses revealed that the rhizosphere microbiome of the Fusarium-resistant accession was distinctly different from that of the Fusarium-susceptible accession, with higher consumption of amino acids and amines, higher metabolism of xylanase and sialidase, and higher expression of genes associated with nitrogen, phosphorus and iron metabolism. The resistome analysis indicates higher expression of soxR, which is involved in protecting bacteria against oxidative stress induced by a pathogen invasion. These results further support our hypothesis that breeding for resistance has unintentionally shaped the assembly and activity of the rhizobacterial community toward a higher abundance of specific rhizosphere competent bacterial taxa that can provide complementary protection against fungal root infections. 650 $aBeans 650 $aCarbohydrate metabolism 650 $aDisease resistance 650 $aMicrobiome 650 $aSoil-plant interactions 650 $aFeijão 650 $aMicrobiologia do Solo 650 $aPopulação Microbiana 650 $aResistência 653 $aBiolog EcoPlate 653 $aMetatranscriptome 653 $aNutrientmetabolism 653 $aPlant-microbe interactions 653 $aResistome 700 1 $aCHAVES, M. G. de 700 1 $aFONSECA, M. de C. 700 1 $aMENDES, R. 700 1 $aRAAIJMAKERS, J. M. 700 1 $aTSAI, S. M. 773 $tFrontiers in Microbiology$gv. 10, 2019. Article 2252.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
24/10/2018 |
Data da última atualização: |
24/10/2018 |
Autoria: |
BORGES, A. A.; SANTOS, M. V. O.; QUEIROZ NETA, L. B.; OLIVEIR, A M. F.; SILVA, A. R.; PEREIRA, A. F. |
Afiliação: |
Alana A. Borges, Laboratório de Biotecnologia Animal/Universidade Federal Rural do Semi-Árido - UFERSA; Maria V. O. Santos, Laboratório de Biotecnologia Animal/Universidade Federal Rural do Semi-Árido - UFERSA; Luiza B. Queiroz Neta, Laboratório de Biotecnologia Animal/Universidade Federal Rural do Semi-Árido - UFERSA; Moacir F. Oliveira, Laboratório de Morfofisiologia Animal Aplicada/Universidade Federal Rural do Semi-Árido - UFERSA; Alexandre R. Silva, Laboratório de Conservação de Germoplasma Animal/ Universidade Federal Rural do Semi-Árido - UFERSA; Alexsandra F. Pereira, Laboratório de Biotecnologia Animal/Universidade Federal Rural do Semi-Árido - UFERSA. |
Título: |
In vitro maturation of collared peccary (Pecari tajacu) oocytes after different incubation times. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 9, p. 1863-1868, setembro 2018 |
Idioma: |
Inglês |
Notas: |
Título em português: Maturação in vitro de oócitos de cateto (Pecari tajacu) após diferentes períodos de incubação. |
Conteúdo: |
Oocyte in vitro maturation (IVM) is the first step of the in vitro reproductive technologies that enables mature oocytes to be generated ex vivo and after used for embryo production. In this sense, the establishment of culture environment, as oocyte incubation time, is essential for the success of the IVM. Therefore, the study was carried out to investigate the relationship between the meiotic potential and the IVM times of collared peccary oocytes, wild mammals of great commercial and ecological interest. Thus, ovaries were collected of females derived from captivity and transported to the laboratory within 1 hour of slaughtering. The oocytes derived from follicles (3-6mm in diameter) were recovered by aspirated and sliced. Good quality oocytes (evenly granulated cytoplasm with a least one layer of surrounding cumulus cells) were selected and subjected to culture in TCM 199 supplemented with 10?g/mL FSH, 10% FBS and 100?M cysteamine at 38.5°C, 5% CO2 and maximum humidity for 24 or 48 hours. After the incubation period, the nuclear status, the presence of first polar body and the expansion of cumulus cells of oocytes were assessed. The data obtained were analyzed by Fisher exact test (P<0.05). A total of four sessions (2-3 females per session) were performed, resulting in eighteen aspirated and sliced ovaries with normal morphological characteristics. An oocyte recovery rate of about 83.1% (59/71) was obtained with 3.3 oocytes/ovary and 2.3 viable oocytes/ovary. After different incubation times, differences (P<0.05) were observed in 24 and 48 hours for expansion of the cumulus cells (38.1% vs. 100%), presence of first polar body (52.4% vs. 90.5%) and nuclear status in second metaphase (19.0% vs. 76.2%), respectively. In conclusion, 48 hours is suitable time for the in vitro maturation of oocytes derived from collared peccaries when compared to the time of 24 hours, according to the meiotic potential observed. Additional studies should be conducted to improve the quality of the oocyte culture environment, as medium composition, aiming to obtain viable mature oocytes for other in vitro biotechnologies. MenosOocyte in vitro maturation (IVM) is the first step of the in vitro reproductive technologies that enables mature oocytes to be generated ex vivo and after used for embryo production. In this sense, the establishment of culture environment, as oocyte incubation time, is essential for the success of the IVM. Therefore, the study was carried out to investigate the relationship between the meiotic potential and the IVM times of collared peccary oocytes, wild mammals of great commercial and ecological interest. Thus, ovaries were collected of females derived from captivity and transported to the laboratory within 1 hour of slaughtering. The oocytes derived from follicles (3-6mm in diameter) were recovered by aspirated and sliced. Good quality oocytes (evenly granulated cytoplasm with a least one layer of surrounding cumulus cells) were selected and subjected to culture in TCM 199 supplemented with 10?g/mL FSH, 10% FBS and 100?M cysteamine at 38.5°C, 5% CO2 and maximum humidity for 24 or 48 hours. After the incubation period, the nuclear status, the presence of first polar body and the expansion of cumulus cells of oocytes were assessed. The data obtained were analyzed by Fisher exact test (P<0.05). A total of four sessions (2-3 females per session) were performed, resulting in eighteen aspirated and sliced ovaries with normal morphological characteristics. An oocyte recovery rate of about 83.1% (59/71) was obtained with 3.3 oocytes/ovary and 2.3 viable oocytes/ovary. After differ... Mostrar Tudo |
Palavras-Chave: |
Cateto; Collared peccary; Competência meiótica; Incubation; Maturação nuclear; Meiotic competence; Nuclear maturation; Oócito; Pecari tajacu; Wild mammals. |
Thesagro: |
Incubação; Maturação; Reprodução. |
Thesaurus NAL: |
Oocytes; Reproduction. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/185059/1/In-vitro-maturation-of-collared-peccary.pdf
|
Marc: |
LEADER 03328naa a2200373 a 4500 001 2098144 005 2018-10-24 008 2018 bl uuuu u00u1 u #d 100 1 $aBORGES, A. A. 245 $aIn vitro maturation of collared peccary (Pecari tajacu) oocytes after different incubation times.$h[electronic resource] 260 $c2018 500 $aTítulo em português: Maturação in vitro de oócitos de cateto (Pecari tajacu) após diferentes períodos de incubação. 520 $aOocyte in vitro maturation (IVM) is the first step of the in vitro reproductive technologies that enables mature oocytes to be generated ex vivo and after used for embryo production. In this sense, the establishment of culture environment, as oocyte incubation time, is essential for the success of the IVM. Therefore, the study was carried out to investigate the relationship between the meiotic potential and the IVM times of collared peccary oocytes, wild mammals of great commercial and ecological interest. Thus, ovaries were collected of females derived from captivity and transported to the laboratory within 1 hour of slaughtering. The oocytes derived from follicles (3-6mm in diameter) were recovered by aspirated and sliced. Good quality oocytes (evenly granulated cytoplasm with a least one layer of surrounding cumulus cells) were selected and subjected to culture in TCM 199 supplemented with 10?g/mL FSH, 10% FBS and 100?M cysteamine at 38.5°C, 5% CO2 and maximum humidity for 24 or 48 hours. After the incubation period, the nuclear status, the presence of first polar body and the expansion of cumulus cells of oocytes were assessed. The data obtained were analyzed by Fisher exact test (P<0.05). A total of four sessions (2-3 females per session) were performed, resulting in eighteen aspirated and sliced ovaries with normal morphological characteristics. An oocyte recovery rate of about 83.1% (59/71) was obtained with 3.3 oocytes/ovary and 2.3 viable oocytes/ovary. After different incubation times, differences (P<0.05) were observed in 24 and 48 hours for expansion of the cumulus cells (38.1% vs. 100%), presence of first polar body (52.4% vs. 90.5%) and nuclear status in second metaphase (19.0% vs. 76.2%), respectively. In conclusion, 48 hours is suitable time for the in vitro maturation of oocytes derived from collared peccaries when compared to the time of 24 hours, according to the meiotic potential observed. Additional studies should be conducted to improve the quality of the oocyte culture environment, as medium composition, aiming to obtain viable mature oocytes for other in vitro biotechnologies. 650 $aOocytes 650 $aReproduction 650 $aIncubação 650 $aMaturação 650 $aReprodução 653 $aCateto 653 $aCollared peccary 653 $aCompetência meiótica 653 $aIncubation 653 $aMaturação nuclear 653 $aMeiotic competence 653 $aNuclear maturation 653 $aOócito 653 $aPecari tajacu 653 $aWild mammals 700 1 $aSANTOS, M. V. O. 700 1 $aQUEIROZ NETA, L. B. 700 1 $aOLIVEIR, A M. F. 700 1 $aSILVA, A. R. 700 1 $aPEREIRA, A. F. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 9, p. 1863-1868, setembro 2018
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|