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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
24/11/2014 |
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Data da última atualização: |
24/11/2014 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CHAVES, F. C. M.; CUNHA, A. L. B. da; BATISTA, A. C.; HIDALGO, A. de F. |
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Afiliação: |
FRANCISCO CELIO MAIA CHAVES, CPAA. |
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Título: |
Propagação por estaquia de Piper hispidum Sw., Piper marginatum Jacq. e Piper tuberculatum Jacq. em Manaus-AM. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2014. |
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Idioma: |
Português |
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Palavras-Chave: |
Espécies nativas; Propagação assexuada. |
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Thesagro: |
Piperaceae; Sobrevivência. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112197/1/ResumoCBRG-758.pdf
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Marc: |
LEADER 00695nam a2200181 a 4500
001 2000796
005 2014-11-24
008 2014 bl uuuu u00u1 u #d
100 1 $aCHAVES, F. C. M.
245 $aPropagação por estaquia de Piper hispidum Sw., Piper marginatum Jacq. e Piper tuberculatum Jacq. em Manaus-AM.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos$c2014
650 $aPiperaceae
650 $aSobrevivência
653 $aEspécies nativas
653 $aPropagação assexuada
700 1 $aCUNHA, A. L. B. da
700 1 $aBATISTA, A. C.
700 1 $aHIDALGO, A. de F.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
15/02/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
SANTOS, M. L. T.; BORGES, A. A.; QUEIROZ NETA, L. B.; SANTOS, M. V. O.; OLIVEIRA, M. F.; SILVA, A. R.; PEREIRA, A. F. |
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Afiliação: |
MAGDA L. T. SANTOS, UFERSA; ALANA A. BORGES, UFERSA; LUIZA B. QUEIROZ NETA, UFERSA; MARIA V. O. SANTOS, UFERSA; MOACIR F. OLIVEIRA, UFERSA; ALEXANDRE R. SILVA, UFERSA; ALEXANDRA F. PEREIRA, UFERSA. |
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Título: |
In vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 12, p. 1194-1202, dez. 2016. |
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Idioma: |
Português |
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Conteúdo: |
The maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries. MenosThe maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve th... Mostrar Tudo |
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Palavras-Chave: |
Mitotic factor; Somatic tissue. |
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Thesagro: |
Tayassu Tajacu. |
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Thesaurus NAL: |
Culture media; Protein sources; Somatic cells; Wild animals. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155804/1/In-vitro-culutre-of-somatic.pdf
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Marc: |
LEADER 02511naa a2200277 a 4500
001 2064367
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTOS, M. L. T.
245 $aIn vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements.$h[electronic resource]
260 $c2016
520 $aThe maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries.
650 $aCulture media
650 $aProtein sources
650 $aSomatic cells
650 $aWild animals
650 $aTayassu Tajacu
653 $aMitotic factor
653 $aSomatic tissue
700 1 $aBORGES, A. A.
700 1 $aQUEIROZ NETA, L. B.
700 1 $aSANTOS, M. V. O.
700 1 $aOLIVEIRA, M. F.
700 1 $aSILVA, A. R.
700 1 $aPEREIRA, A. F.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 12, p. 1194-1202, dez. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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