Data/hora: 04/06/2024 07:07:51

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 30/06/2004
Data da última atualização: | 30/03/2023
Autoria: | QUEIROZ, P. R.; INGLIS, M. C. V.; INGLIS, P. W.
Afiliação: | Paulo Roberto Queiroz, CENARGEN; MARIA CLERIA VALADARES INGLIS, Cenargen; Peter Ward Inglis, CPAC.
Título: | Survival in soil and detection of co-transformed Trichoderma harzianum by nested PCR.
Ano de publicação: | 2004
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 4, p.403-405, abr. 2004
Idioma: | Inglês

Notas:

Notas Científicas.
Título em português: Sobrevivência em solo e detecção de co-transformantes de Trichoderma harzianum por PCR ?nested?.

Conteúdo: | The objective of this work was to evaluate the survival of two Trichoderma harzianum co-transformants, TE 10 and TE 41, carrying genes for green fluorescent protein (egfp) and for resistance to benomyl, during four weeks in a contained soil microcosm. Selective culture media were used to detect viable fungal material, whose identity was confirmed by the observation of the fluorescent phenotype by direct epifluorence microscopy. PCR using two nested primer pairs specific to the egfp gene was also used to detect the transformed fungi. Although it was not possible to reliably detect the egfp gene directly from soil extracts, an enrichment step involving selective culture of soil samples in liquid medium prior to DNA extraction enabled the consistent detection of the T. harzianum co-transformants by nested PCR for the duration of the incubation period.
Palavras-Chave: | benomyl resistance; egfp; resistência a benomil; transformação; transformation.
Thesagro: | Controle Biológico.
Thesaurus Nal: | biological control.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/26694/1/39n04a15.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (26694-1) - UPE - AP - DD - 630.72081 - P474 -  - DIS