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| Registros recuperados : 18 | |
| 2. |  | LANNA FILHO, R.; SOUZA, R. M.; FERREIRA, A.; QUECINE, M. C.; ALVES, E.; AZEVEDO, J. L. de. Biocontrol activity of Bacillus against a GFP-marked Pseudomonas syringae pv. tomato on tomato phylloplane. Australasian Plant Pathology, Holanda, v. 42, n. 6, p. 643-651, nov. 2013 42 643-651| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 4. | | LACAVA, P. T.; DIAS, A.; SANTOS, V. C.; FERREIRA, A.; QUECINE, M. C.; ARAÚJO, W. L.; MELO, I. S. de; AZEVEDO, J. L. Analysis and characterization of the bacteria community associated with tropical mangrove forest. In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 13., 2007, Sorrento. Book of abstracts... Sorrento, 2007. p.238.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 5. | | GUIMARÃES, J. P. S. M.; RODRIGUES, K. B.; SOUZA, B. G. de; BRANDÃO, L. T. D.; QUECINE, M. C.; FAVARO, L. C. de L. Characterization of Bacillus, Paenibacillus, Peribacillus, and Alkalihalobacillus strains for indole-3-acetic acid production. In: INTERNATIONAL SYMPOSIUM OF MICROBIOLOGY AND BIOTECHNOLOGY, 6., 2023, Viçosa, MG. Biotecnologia Microbiana Soluções para os grandes desafios globais: anais. Viçosa, MG: Universidade Federal de Viçosa, 2023. Resumo.| Biblioteca(s): Embrapa Agroenergia. |
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| 6. | | FREIRE, Í. A.; MENDES, T. D.; MIDORIKAWA, G. E. O.; SILVA, C. P. da; QUECINE, M. C.; FAVARO, L. C. de L. Harnessing the potential of native Bacillus species for mineralizing organic sources of phosphorus. In: INTERNATIONAL SYMPOSIUM OF MICROBIOLOGY AND BIOTECHNOLOGY, 6., 2023, Viçosa, MG. Biotecnologia Microbiana Soluções para os grandes desafios globais: anais. Viçosa, MG: Universidade Federal de Viçosa, 2023. Resumo.| Biblioteca(s): Embrapa Agroenergia. |
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| 7. | | SIQUEIRA, A. C. O.; MASCARIN, G. M.; GONÇALVES, C. R. N. C. B.; MARCON, J.; QUECINE, M. C.; FIGUEIRA, A.; DELALIBERA JUNIOR, Í. Multi-trait biochemical features of Metarhizium species and their activities that stimulate the growth of tomato plants. Frontiers in Sustainable Food Systems, v. 4, article 137, 2020.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 8. | | CASTRO, R. A.; QUECINE, M. C.; LACAVA, P. T.; BATISTA, B. D.; LUVIZOTTO, D. M.; MARCON, J.; FERREIRA, A.; MELO, I. S. de; AZEVEDO, J. L. Isolation and enzyme bioprospection of endophytic bacteria associated with plants of Brazilian mangrove ecosystem. SpringerPlus, Amsterdam, v. 3, n. 382, p. 1-9, 2014.| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 9. | | CASTRO, R. A.; QUECINE, M. C.; LACAVA, P. T.; BATISTA, B. D.; LUVIZOTTO, D. M.; MARCON, J.; FERREIRA, A.; MELO, I. S. de; AZEVEDO, J. L. Isolation and enzyme bioprospection of endophytic bacteria associated with plants of Brazilian mangrove ecosystem. SpringerPlus, Amsterdam, v. 3, n. 382, p. 1-9, 2014.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 10. | | CASTRO, R. A.; DOURADO, M. N.; ALMEIDA, J. R. de; LACAVA, P. T.; NAVE, A.; MELO, I. S. de; AZEVEDO, J. L. de; QUECINE, M. C. Mangrove endophyte promotes reforestation tree (Acacia polyphylla) growth. Brazilian Journal of Microbiology, v. 49, n. 1, p. 59-66, 2018.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 11. | | SÁ, A. L. B.; DIAS, A. C. F.; QUECINE, M. C.; COTTA, S. R.; FASANELLA, C. C.; ANDREOTE, F. D.; MELO, I. S. de. Screening of endoglucanase-producing bacteria in the saline rhizosphere of Rhizophora mangle. Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 193-197, 2014.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | Quecine, M. C.; Araújo, W. L.; Rosseto, P. B.; FERREIRA, A.; TSUI, S.; Lacava, P. T.; Mondin, M.; Azevedo, J. L.; Pizzirani-Kleiner, A. A. Sugarcane growth promotion by the endophytic bacterium Pantoea agglomerans 33.1. Applied and Environmental Microbiology, v. 78, n. 21, p. 7511-7518, 2012.| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 13. | | NUNES, L. S.; FREIRE, Í. A.; RODRIGUES, K. B.; MIDORIKAWA, G. E. O.; QUECINE, M. C.; FAVARO, L. C. de L. In vitro assessment of inorganic phosphate-solubilizing potential of Bacillus, Paenibacillus, Peribacillus, and Alkalihalobacillus strains. In: INTERNATIONAL SYMPOSIUM OF MICROBIOLOGY AND BIOTECHNOLOGY, 6., 2023, Viçosa, MG. Biotecnologia Microbiana Soluções para os grandes desafios globais: anais. Viçosa, MG: Universidade Federal de Viçosa, 2023. Resumo.| Biblioteca(s): Embrapa Agroenergia. |
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| 14. | | LIMA, L. B. M.; MENDES, T. D.; QUECINE, M. C.; ARAÚJO, W. L.; JARAMILLO, P. M. D.; QUIRINO, B. F.; RODRIGUES, D. de S.; SALUM, T. F. C.; FAVARO, L. C. de L. Bactérias associadas a plantas e seu potencial para produção de enzimas lignocelulolíticas. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos, SP. [Anais ...]. Brasília, DF: SBRG, 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 15. | | FAVARO, L. C. de L.; MELO, I. S.; QUIRINO, B. F.; OLIVEIRA, A. C. B.; MENDES, T. D.; SALUM, T. F. C.; RODRIGUES, D. de S.; SOUZA, G. P.; FRANCO, P. F.; QUECINE, M. C. Brazilian microbial diversity as a source of new strains for deconstruction of lignocellulosic biomass. Hechos Microbiológicos, v. 5, n. 2, suplemento 2, 2014. Trabalho apresentado no XXII Congresso Latinoamericano de Microbiología - ALAM 2014 e 4 Congresso Colombiano de Microbiología - 4 CCM 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 16. | | FAVARO, L. C. de L.; MELO, I. S. de; QUIRINO, B. F.; OLIVEIRA, A. C. B.; MENDES, T. D.; SALUM, T. F. C.; RODRIGUES, D. S.; SOUZA, G. P.; FRANCO, P. F.; QUECINE, M. C. Brazilian microbial diversity as a source of new strains for deconstruction of lignocellulosic biomass. Hechos Microbiológicos, v. 5, n. 2, p. 133, 2014. Suplemento. Edição das Memorias do 22º Congreso Latinoamericano de Microbiologia e 4º Congreso Colombiano de Microbiologia, Cartagena, 2014. Ref. TLP-310.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 17. | | LIMA, L. B. M.; CARATTI, M. F.; SILVA, A. C. M. G.; SALUM, T. F. C.; ARAÚJO, W. L.; QUECINE, M. C.; QUIRINO, B. F.; FAVARO, L. C. de L. Prospecção de bactérias endofíticas de plantas nativas e cultivadas e seu potencial para a desconstrução de biomassa lignocelulósica. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2015. p. 51-53| Biblioteca(s): Embrapa Agroenergia. |
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| 18. | | LIMA, L. B. M.; CARATTI, M. F.; SILVA, A. C. M. G.; SALUM, T. F. C.; ARAÚJO, W. L.; QUECINE, M. C.; QUIRINO, B. F.; FAVARO, L. C. de L. Prospecção de bactérias endofíticas e seu potencial para a desconstrução de biomassa lignocelulósica. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA A AMÉRICA LATINA E CARIBE, 10., 2015, Bento Gonçalves. Recursos genéticos no século 21: de Vavilov a Svalbard. Anais... [s.l.]: Sociedade Brasileira de Recursos Genéticos, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 18 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
03/02/2014 |
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Data da última atualização: |
03/02/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
LANNA FILHO, R.; SOUZA, R. M.; FERREIRA, A.; QUECINE, M. C.; ALVES, E.; AZEVEDO, J. L. de. |
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Afiliação: |
Roberto Lanna Filho, UFRGS; Ricardo Magela de Souza, UFLA; ANDERSON FERREIRA, CPAMT; Maria Carolina Quecine Verdi, USP-ESALQ; Eduardo Alves, UFLA; USP-ESALQ. |
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Título: |
Biocontrol activity of Bacillus against a GFP-marked Pseudomonas syringae pv. tomato on tomato phylloplane. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Australasian Plant Pathology, Holanda, v. 42, n. 6, p. 643-651, nov. 2013 |
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Volume: |
42 |
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Páginas: |
643-651 |
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ISSN: |
0815-3191 |
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DOI: |
http://dx.doi.org/10.1007/s13313-013-0233-z |
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Idioma: |
Inglês |
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Conteúdo: |
We report the biocontrol activity of the endophytic bacteria Bacillus pumilus and Bacillus amyloliquefacies against the plant pathogenic bacteriumPseudomonas syringae pv.tomato strain NS4 transformed with theGFPexpressing gene. P. s . pv. tomato strain NS4 was obtained from the transformation of P. s .pv.tomato wild-type strain NW with the plasmid pNKGFP containing GFP-cassette for chromosomal integration. The GFP-marked strain was tested for hypersensitivity and pathogenicity, as well as population studies on the phylloplane, to determine its epidemiology and survival. In all of the bioassays strain NS4 presented similar characteristics to the wild-type, and was hence chosen as the model strain for these studies with antagonistic endophytic bacterial strains. In the biocontrol experiments, tomato plants were preinoculated with the endophytic bacteria 4 days prior to inoculation withP. s .pv.tomatostrains. On the tomato phylloplane the P. s .pv.tomato (strains NW and NS4) populations were drastically reduced, and tomato leaves showed reduced numbers of bacterial speck lesions, comparable to the standard chemical treatment copper oxychloride. Additionally, under epifluorescence microscopy, few GFP-tagged cells of strain NS4 were observed colonizing important niches on the tomato phylloplane. However, leaves untreated with the antagonists presented a large number of GFP-tagged cell aggregates. Our results demonstrated that endophytic bacteria can also act efficiently on the biocontrol of bacterial speck when applied as a foliar spray on the leaves. In addition, we highlighted the use of GFP-marked strain NS4 as a model system to study biocontrol agent and pathogen interactions, and growth and development of the pathogen on the tomato leaf surface. MenosWe report the biocontrol activity of the endophytic bacteria Bacillus pumilus and Bacillus amyloliquefacies against the plant pathogenic bacteriumPseudomonas syringae pv.tomato strain NS4 transformed with theGFPexpressing gene. P. s . pv. tomato strain NS4 was obtained from the transformation of P. s .pv.tomato wild-type strain NW with the plasmid pNKGFP containing GFP-cassette for chromosomal integration. The GFP-marked strain was tested for hypersensitivity and pathogenicity, as well as population studies on the phylloplane, to determine its epidemiology and survival. In all of the bioassays strain NS4 presented similar characteristics to the wild-type, and was hence chosen as the model strain for these studies with antagonistic endophytic bacterial strains. In the biocontrol experiments, tomato plants were preinoculated with the endophytic bacteria 4 days prior to inoculation withP. s .pv.tomatostrains. On the tomato phylloplane the P. s .pv.tomato (strains NW and NS4) populations were drastically reduced, and tomato leaves showed reduced numbers of bacterial speck lesions, comparable to the standard chemical treatment copper oxychloride. Additionally, under epifluorescence microscopy, few GFP-tagged cells of strain NS4 were observed colonizing important niches on the tomato phylloplane. However, leaves untreated with the antagonists presented a large number of GFP-tagged cell aggregates. Our results demonstrated that endophytic bacteria can also act efficiently on the bi... Mostrar Tudo |
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Palavras-Chave: |
Bacterial Speck; Endophytic Bactéria. |
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Thesaurus NAL: |
Bacillus amyloliquefaciens; Bacillus pumilus; biological control; green fluorescent protein. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/96286/1/cpamt-ferreira-0815-3191-2013.pdf
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Marc: |
LEADER 02712naa a2200301 a 4500
001 1978335
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008 2013 bl uuuu u00u1 u #d
022 $a0815-3191
024 7 $ahttp://dx.doi.org/10.1007/s13313-013-0233-z$2DOI
100 1 $aLANNA FILHO, R.
245 $aBiocontrol activity of Bacillus against a GFP-marked Pseudomonas syringae pv. tomato on tomato phylloplane.$h[electronic resource]
260 $c2013
300 $a643-651 42
490 $v42
520 $aWe report the biocontrol activity of the endophytic bacteria Bacillus pumilus and Bacillus amyloliquefacies against the plant pathogenic bacteriumPseudomonas syringae pv.tomato strain NS4 transformed with theGFPexpressing gene. P. s . pv. tomato strain NS4 was obtained from the transformation of P. s .pv.tomato wild-type strain NW with the plasmid pNKGFP containing GFP-cassette for chromosomal integration. The GFP-marked strain was tested for hypersensitivity and pathogenicity, as well as population studies on the phylloplane, to determine its epidemiology and survival. In all of the bioassays strain NS4 presented similar characteristics to the wild-type, and was hence chosen as the model strain for these studies with antagonistic endophytic bacterial strains. In the biocontrol experiments, tomato plants were preinoculated with the endophytic bacteria 4 days prior to inoculation withP. s .pv.tomatostrains. On the tomato phylloplane the P. s .pv.tomato (strains NW and NS4) populations were drastically reduced, and tomato leaves showed reduced numbers of bacterial speck lesions, comparable to the standard chemical treatment copper oxychloride. Additionally, under epifluorescence microscopy, few GFP-tagged cells of strain NS4 were observed colonizing important niches on the tomato phylloplane. However, leaves untreated with the antagonists presented a large number of GFP-tagged cell aggregates. Our results demonstrated that endophytic bacteria can also act efficiently on the biocontrol of bacterial speck when applied as a foliar spray on the leaves. In addition, we highlighted the use of GFP-marked strain NS4 as a model system to study biocontrol agent and pathogen interactions, and growth and development of the pathogen on the tomato leaf surface.
650 $aBacillus amyloliquefaciens
650 $aBacillus pumilus
650 $abiological control
650 $agreen fluorescent protein
653 $aBacterial Speck
653 $aEndophytic Bactéria
700 1 $aSOUZA, R. M.
700 1 $aFERREIRA, A.
700 1 $aQUECINE, M. C.
700 1 $aALVES, E.
700 1 $aAZEVEDO, J. L. de
773 $tAustralasian Plant Pathology, Holanda$gv. 42, n. 6, p. 643-651, nov. 2013
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