Data/hora: 06/06/2024 02:23:12

Biblioteca(s): | Embrapa Agricultura Digital.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 21/11/2012
Data da última atualização: | 14/04/2020
Autoria: | PROTASIO, A. V.; TSAI, I. J.; BABBAGE, A.; NICHOL, S.; HUNT, M.; ASLETT, M. A.; SILVA, N. de; VELARDE, G. S.; ANDERSON, T. J. C.; CLARK, R. C.; DAVIDSON, C.; DILLON, G. P.; HOLROYD, N. E.; LOVERDE, P. T.; LLOYD, C.; MCQUILLAN, J.; OLIVEIRA, G.; OTTO, T. D.; PARKER-MANUEL, S. J.; QUAIL, M. A.; WILSON, R. A.; ZERLOTINI, A.; DUNNE, D. W.; BERRIMAN, M.
Afiliação: | ANNA V. PROTASIO, Wellcome Trust Sanger Institute; ISHENG J. TSAI, Wellcome Trust Sanger Institute; ANNE BABBAGE, Wellcome Trust Sanger Institute; SARAH NICHOL, Wellcome Trust Sanger Institute; MARTIN HUNT, Wellcome Trust Sanger Institute; MARTIN A. ASLETT, Wellcome Trust Sanger Institute; NISHADI DE SILVA, Wellcome Trust Sanger Institute; GILES S. VELARDE, Wellcome Trust Sanger Institute; TIM J. C. ANDERSON, Texas Biomedical Research Institute; RICHARD C. CLARK, Wellcome Trust Sanger Institute; CLAIRE DAVIDSON, Wellcome Trust Sanger Institute; GARY P. DILLON, Wellcome Trust Sanger Institute; NANCY E. HOLROYD, Wellcome Trust Sanger Institute; PHILIP T. LOVERDE, University of Texas Health Science Center; CHRISTINE LLOYD, Wellcome Trust Sanger Institute; JACQUELLINE MCQUILLAN, Wellcome Trust Sanger Institute; GUILHERME OLIVEIRA, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz; THOMAS D. OTTO, Wellcome Trust Sanger Institute; SOPHIA J. PARKER-MANUEL, University of York; MICHAEL A. QUAIL, Wellcome Trust Sanger Institute; R. ALAN WILSON, University of York; ADHEMAR ZERLOTINI NETO, CNPTIA; DAVID W. DUNNE, University of Cambridge; MATTHEW BERRIMAN, Wellcome Trust Sanger Institute.
Título: | A systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni.
Ano de publicação: | 2012
Fonte/Imprenta: | PLOS Neglected Tropical Diseases, v. 6, n. 1, p. 1-13, Jan. 2012.
DOI: | 10.1371/journal.pntd.0001455
Idioma: | Inglês
Conteúdo: | Schistosomiasis is one of the most prevalent parasitic diseases, affecting millions of people in developing countries. Amongst the human-infective species, Schistosoma mansoni is also the most commonly used in the laboratory and here we present the systematic improvement of its draft genome. We used Sanger capillary and deep-coverage Illumina sequencing from clonal worms to upgrade the highly fragmented draft 380 Mb genome to one with only 885 scaffolds and more than 81% of the bases organised into chromosomes. We have also used transcriptome sequencing (RNA-seq) from four time points in the parasite?s life cycle to refine gene predictions and profile their expression. More than 45% of predicted genes have been extensively modified and the total number has been reduced from 11,807 to 10,852. Using the new version of the genome, we identified trans-splicing events occurring in at least 11% of genes and identified clear cases where it is used to resolve polycistronic transcripts. We have produced a high-resolution map of temporal changes in expression for 9,535 genes, covering an unprecedented dynamic range for this organism. All of these data have been consolidated into a searchable format within the GeneDB (www.genedb.org) and SchistoDB (www.schistodb.net) databases. With further transcriptional profiling and genome sequencing increasingly accessible, the upgraded genome will form a fundamental dataset to underpin further advances in schistosome research.
Thesaurus Nal: | Schistosoma.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/70531/1/journal.pntd.0001455.pdf
Registro original: | Embrapa Agricultura Digital (CNPTIA)
Exemplares: | CNPTIA - (16945-1) - UPC - AP - DD -  -  -  - DIS