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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
13/09/2011 |
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Data da última atualização: |
04/10/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
PÁDUA, G. P.; JESUS, A. M. S.; FRONZA, V.; ARANTES, N. E.; ZITO, R. K. |
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Afiliação: |
GILDA PIZZOLANTE DE PADUA, EPAMIG; A. M. S. JESUS, EPAMIG; VANOLI FRONZA, CNPSO; NEYLSON EUSTÁQUIO ARANTES, Fundação Triângulo; ROBERTO KAZUHIKO ZITO, CNPSO. |
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Título: |
Comparação entre bio e imunoensaio para identificação de sementes de soja geneticamente modificada tolerante ao glifosato em lotes de sementes de soja não transgênica. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 32., 2011, São Pedro, SP. Resumos expandidos... Londrina: Embrapa Soja, 2011. p. 339-341. Editado por Adilson de Oliveira Junior, Odilon Ferreira Saraiva, Regina Maria Villas Bôas de Campos Leite. |
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Idioma: |
Português |
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Thesagro: |
Semente; Soja. |
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Thesaurus Nal: |
Seeds; Soybeans. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/41908/1/paduap.339-341.pdf
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Marc: |
LEADER 00836nam a2200193 a 4500
001 1900284
005 2011-10-04
008 2011 bl uuuu u00u1 u #d
100 1 $aPÁDUA, G. P.
245 $aComparação entre bio e imunoensaio para identificação de sementes de soja geneticamente modificada tolerante ao glifosato em lotes de sementes de soja não transgênica.
260 $aIn: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 32., 2011, São Pedro, SP. Resumos expandidos... Londrina: Embrapa Soja, 2011. p. 339-341. Editado por Adilson de Oliveira Junior, Odilon Ferreira Saraiva, Regina Maria Villas Bôas de Campos Leite.$c2011
650 $aSeeds
650 $aSoybeans
650 $aSemente
650 $aSoja
700 1 $aJESUS, A. M. S.
700 1 $aFRONZA, V.
700 1 $aARANTES, N. E.
700 1 $aZITO, R. K.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/12/2023 |
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Data da última atualização: |
11/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, J. R. da; SILVA, J. F. M.; PEREIRA, M. F.; TORRES, A. R.; GONÇALVES, M. S.; PRATA, M. C. de A.; BRITO, M. A. V. P. e; COSTA, G. M. da; RIBEIRO, J. B. |
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Afiliação: |
JULIANA ROSA DA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; JOICE FÁTIMA MOREIRA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; MONALESSA FÁBIA PEREIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ADALGISA RIBEIRO TORRES; MAYSA SERPA GONÇALVES, UNIVERSIDADE FEDERAL DE LAVRAS; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; GERALDO MÁRCIO DA COSTA, UNIVERSIDADE FEDERAL DE LAVRAS; JOAO BATISTA RIBEIRO, CNPGL. |
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Título: |
Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 55, n. 1, p. 889-900, 2024. |
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DOI: |
https://doi.org/10.1007/s42770-023-01181-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Staphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations. MenosStaphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic tar... Mostrar Tudo |
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Palavras-Chave: |
Infecção intramamária; Modelo de invertebrados; Virulência bacteriana. |
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Thesagro: |
Bovino; Doença Animal. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/262967/1/Is-Galleria-mellonella-model-a-good-alternative-to-study-virulence.pdf
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Marc: |
LEADER 02652naa a2200289 a 4500
001 2159710
005 2024-03-11
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-023-01181-1$2DOI
100 1 $aSILVA, J. R. da
245 $aIs Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?$h[electronic resource]
260 $c2024
520 $aStaphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations.
650 $aBovino
650 $aDoença Animal
653 $aInfecção intramamária
653 $aModelo de invertebrados
653 $aVirulência bacteriana
700 1 $aSILVA, J. F. M.
700 1 $aPEREIRA, M. F.
700 1 $aTORRES, A. R.
700 1 $aGONÇALVES, M. S.
700 1 $aPRATA, M. C. de A.
700 1 $aBRITO, M. A. V. P. e
700 1 $aCOSTA, G. M. da
700 1 $aRIBEIRO, J. B.
773 $tBrazilian Journal of Microbiology$gv. 55, n. 1, p. 889-900, 2024.
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