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Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
02/09/1999 |
Data da última atualização: |
02/09/1999 |
Autoria: |
SILVA, I. S.; MORUA, W. M.; MALUF, W. R. |
Afiliação: |
EMBRAPA-CNPH, Brasilia, DF. |
Título: |
Avaliacao da resistencia ao virus do mosaico da melancia-1 (WMV-1) em introducoes de Cucurbita spp. |
Ano de publicação: |
1984 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE OLERICULTURA, 24.; REUNIAO LATINO-AMERICANA DE OLERICULTURA, 1., 1984, Jaboticabal, SP. Resumos... Jaboticabal: FCAV, 1984. |
Páginas: |
p.89. |
Idioma: |
Português |
Palavras-Chave: |
Abobrinha; Cucurbita ecuadorensis; Moranga; Pumpkin; Resistance; Winter squash; WMV-1. |
Thesagro: |
Abóbora; Cucúrbita Máxima; Cucúrbita Moschata; Cucúrbita Pepo; Resistência; Vírus. |
Thesaurus Nal: |
zucchini. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00933naa a2200313 a 4500 001 1765321 005 1999-09-02 008 1984 bl uuuu u00u1 u #d 100 1 $aSILVA, I. S. 245 $aAvaliacao da resistencia ao virus do mosaico da melancia-1 (WMV-1) em introducoes de Cucurbita spp. 260 $c1984 300 $ap.89. 650 $azucchini 650 $aAbóbora 650 $aCucúrbita Máxima 650 $aCucúrbita Moschata 650 $aCucúrbita Pepo 650 $aResistência 650 $aVírus 653 $aAbobrinha 653 $aCucurbita ecuadorensis 653 $aMoranga 653 $aPumpkin 653 $aResistance 653 $aWinter squash 653 $aWMV-1 700 1 $aMORUA, W. M. 700 1 $aMALUF, W. R. 773 $tIn: CONGRESSO BRASILEIRO DE OLERICULTURA, 24.; REUNIAO LATINO-AMERICANA DE OLERICULTURA, 1., 1984, Jaboticabal, SP. Resumos... Jaboticabal: FCAV, 1984.
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Embrapa Hortaliças (CNPH) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
28/08/2018 |
Data da última atualização: |
24/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
JORJÃO, A. L.; OLIVEIRA, L. D.; SCORZONI, L.; FIGUEIREDO-GODOI, L. M. A.; PRATA, M. C. de A.; JORGE, A. O. C.; JUNQUEIRA, J. C. |
Afiliação: |
Adeline L. Jorjão, UNESP; Luciane D. Oliveira, UNESP; Liliana Scorzoni, UNESP; Lívia Mara A. Figueiredo-Godoi, UNESP; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; Antonio Olavo C. Jorge, UNESP; Juliana C. Junqueira, UNESP. |
Título: |
From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Virulence, v. 9, n. 1, p. 383-389, 2018. |
Idioma: |
Inglês |
Conteúdo: |
ABSTRACT Galleria mellonella is a well-accepted insect model for the study of pathogen-host interactions and antimicrobial compounds. The main advantages of this model include the low cost of maintenance, the fast life cycle, the possibility of using a large number of caterpillars and the innate immune system, which is evolutionarily conserved relative to mammals. Because of these advantages, different research groups have been working to implement the rearing of G. mellonella in laboratory conditions. This protocol describes our experience in the rearing of G. mellonella caterpillars for experimental infection models and the influence of different artificial diets on developmental and physiological parameters. Here, we suggest a diet composition that benefits the life cycle of G. mellonella by accelerating the larval phase length and increasing the caterpillar weight. This diet also stimulated the immune system of G. mellonella by increasing the hemolymph volume and hemocyte concentration. In addition, our rearing protocol generated caterpillars that are more resistant to infection by Staphylococcus aureus, Escherichia coli and Candida albicans. A standard G. mellonella rearing protocol is fundamental to minimize external influences on the results, and this simple and easy protocol can support researchers starting to rear G. mellonella. |
Palavras-Chave: |
Experimental model; In vivo study. |
Thesaurus NAL: |
Galleria mellonella; Rearing. |
Categoria do assunto: |
O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182039/1/Cnpgl-2018-Virulence-Marcia-From-moths.pdf
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Marc: |
LEADER 02089naa a2200241 a 4500 001 2094672 005 2023-01-24 008 2018 bl uuuu u00u1 u #d 100 1 $aJORJÃO, A. L. 245 $aFrom moths to caterpillars$bIdeal conditions for Galleria mellonella rearing for in vivo microbiological studies.$h[electronic resource] 260 $c2018 520 $aABSTRACT Galleria mellonella is a well-accepted insect model for the study of pathogen-host interactions and antimicrobial compounds. The main advantages of this model include the low cost of maintenance, the fast life cycle, the possibility of using a large number of caterpillars and the innate immune system, which is evolutionarily conserved relative to mammals. Because of these advantages, different research groups have been working to implement the rearing of G. mellonella in laboratory conditions. This protocol describes our experience in the rearing of G. mellonella caterpillars for experimental infection models and the influence of different artificial diets on developmental and physiological parameters. Here, we suggest a diet composition that benefits the life cycle of G. mellonella by accelerating the larval phase length and increasing the caterpillar weight. This diet also stimulated the immune system of G. mellonella by increasing the hemolymph volume and hemocyte concentration. In addition, our rearing protocol generated caterpillars that are more resistant to infection by Staphylococcus aureus, Escherichia coli and Candida albicans. A standard G. mellonella rearing protocol is fundamental to minimize external influences on the results, and this simple and easy protocol can support researchers starting to rear G. mellonella. 650 $aGalleria mellonella 650 $aRearing 653 $aExperimental model 653 $aIn vivo study 700 1 $aOLIVEIRA, L. D. 700 1 $aSCORZONI, L. 700 1 $aFIGUEIREDO-GODOI, L. M. A. 700 1 $aPRATA, M. C. de A. 700 1 $aJORGE, A. O. C. 700 1 $aJUNQUEIRA, J. C. 773 $tVirulence$gv. 9, n. 1, p. 383-389, 2018.
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