Data/hora: 23/09/2024 02:41:59

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	12/08/2021	
Data da última atualização: 	12/08/2021	
Autoria: 	MONTEIRO, C.; COELHO, L.; PAULA, L. G. F. de; FERNANDES, E. K. K.; DOLINSKIC, C.; BITTENCOURT, V. R. E. P.; FURLONGE, J.; PRATA, M. C. de A.	
Afiliação: 	CAIO MONTEIRO, Universidade Federal de Goiás; LETÍCIA COELHO, Universidade Federal de Goiás; LUIZA GABRIELLA FERREIRA DE PAULA, Universidade Federal de Goiás; ÉVERTON KORT KAMP FERNANDES, Universidade Federal de Goiás; CLAUDIA DOLINSKIC, Universidade Estadual Norte Fluminense Darcy Ribeiro; VÂNIA RITA ELIAS PINHEIRO BITTENCOURT, Universidade Federal Rural do Rio de Janeiro; JOHN FURLONGE; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL.	
Título: 	Efficacy of entomopathogenic nematodes in insect cadaver formulation against engorged females of Rhipicephalus microplus (Acari: Ixodidae) in semi-field conditions.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Ticks and Tick-borne Diseases, v. 11, 101313, 2020.	
DOI: 	https://doi.org/10.1016/j.ttbdis.2019.101313	
Idioma: 	Inglês	
Conteúdo: 	The present study evaluated, in the laboratory, the virulence of Heterorhabditis bacteriophora LPP30 against engorged females of Rhipicephalus microplus; in addition, we evaluated, in semi-field tests, the effects of four isolates of Heterorhabditis spp. (i.e., H. bacteriophora HP88, Heterorhabditis baujardi LPP7, Heterorhabditis indica LPP1 and H. bacteriophora LPP30) in insect cadaver formulation against the non-parasitic phase of R. microplus. In the first experiment (in vitro), engorged females were exposed, in Petri dish, to H. bacteriophora LPP30 at 75, 150, 300, 600, 1200 and 2400 nematodes/tick (10 ticks per concentration tested). In the second experiment (semi-field), five engorged females and four cadavers of Galleria mellonella infected with nematodes were placed in pots with soil and grown Brachiaria decumbens; the pots were hold outdoor, exposed to natural environment conditions during the tests. In the third experiment (semi-field), 65 days after the cadavers had been placed in the pots for the second experiment, new engorged females (five per pot) were placed in the pots of the groups treated with H. bacteriophora HP88 or H. baujardi LPP7, to assess their persistence in the soil. In the first test, the percent control was higher than 95% in all treatment groups. In the second experiment, at day 22, the mean mortality rate was 78% in the groups treated with H. bacteriophora LPP30 or H. indica LPP1, and reached 100% and 98% when treated with H. bacteriophora HP88 and H. baujardi LPP7, respectively. In this experiment, the egg-laying inhibition index was higher than 90% in the groups treated with H. bacteriophora HP88 (97.2%) or H. baujardi LPP7 (91.9%). In the third experiment with H. bacteriophora HP88 and H. baujardi LPP7, the egg-laying inhibition index was 59.1% and 43.1%, respectively. We concluded that the isolate LPP30 was highly virulent under laboratory conditions, whereas in semi-field tests, HP88 and LPP7 were the most effective isolates. Moreover, HP88 and LPP7 remained active against engorged females of R. microplus in the soil for 65 days after application of EPN-infected cadavers of G. mellonella.	
Palavras-Chave: 	Heterorhabditis baujardi.	
Thesagro: 	Bovino; Carrapato; Controle Biológico.	
Thesaurus Nal: 	Galleria mellonella; Heterorhabditis bacteriophora; Heterorhabditis indica.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (25187-1) - UPC - AP - DD -  -  -  - DIS