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Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
14/10/2020 |
Data da última atualização: |
12/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CÔNSOLO, N. R. B.; SAMUELSSON, L. M.; BARBOSA, L. C. G. S.; MONARETTO, T.; MORAES, T. B.; BUARQUE, V. L. M.; HIGUERA-PADILLA, A. R.; COLNAGO, L. A.; SILVA, S. L.; REIS, M. M.; FONSECA, A. C.; ARAÚJO, C. S. S.; LEITE, B. G. S.; ROQUE, F. A.; ARAÚJO, L. F. |
Afiliação: |
LUIZ ALBERTO COLNAGO, CNPDIA. |
Título: |
Characterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry: a pilot study. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Poultry Science, v. 99, n. 11, 2020. |
Páginas: |
6247-6257 |
DOI: |
https://doi.org/10.1016/j.psj.2020.06.066 |
Idioma: |
Inglês |
Conteúdo: |
Metabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transverse relaxation time of the breasts determined by TD-NMR relaxometry was shorter for NB than that for WS and WB, indicating greater water mobility in breasts affected by myopathies. 1H-NMR spectroscopy can be used to differentiate the metabolism of WS, WB, and NB, and TD-NMR has the potential to be a fast, simple, and noninvasive method to distinguish NB from WB and WS. As a practical application, the metabolomic profile as per the occurrence of breast myopathies may be used for a better understanding of these issues, which opens a gap to mitigate the incidence and severity of WS and WB. In addition, the present study brings an opportunity for the development of a new and objective tool to classify the incidence of breast myopathies through TD-NMR relaxometry. MenosMetabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transvers... Mostrar Tudo |
Palavras-Chave: |
Chicken breast myopathy; NMR spectroscopy; TD-NMR relaxometry; Water mobilization. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03360naa a2200361 a 4500 001 2125497 005 2022-08-12 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.psj.2020.06.066$2DOI 100 1 $aCÔNSOLO, N. R. B. 245 $aCharacterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry$ba pilot study.$h[electronic resource] 260 $c2020 300 $a6247-6257 520 $aMetabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transverse relaxation time of the breasts determined by TD-NMR relaxometry was shorter for NB than that for WS and WB, indicating greater water mobility in breasts affected by myopathies. 1H-NMR spectroscopy can be used to differentiate the metabolism of WS, WB, and NB, and TD-NMR has the potential to be a fast, simple, and noninvasive method to distinguish NB from WB and WS. As a practical application, the metabolomic profile as per the occurrence of breast myopathies may be used for a better understanding of these issues, which opens a gap to mitigate the incidence and severity of WS and WB. In addition, the present study brings an opportunity for the development of a new and objective tool to classify the incidence of breast myopathies through TD-NMR relaxometry. 653 $aChicken breast myopathy 653 $aNMR spectroscopy 653 $aTD-NMR relaxometry 653 $aWater mobilization 700 1 $aSAMUELSSON, L. M. 700 1 $aBARBOSA, L. C. G. S. 700 1 $aMONARETTO, T. 700 1 $aMORAES, T. B. 700 1 $aBUARQUE, V. L. M. 700 1 $aHIGUERA-PADILLA, A. R. 700 1 $aCOLNAGO, L. A. 700 1 $aSILVA, S. L. 700 1 $aREIS, M. M. 700 1 $aFONSECA, A. C. 700 1 $aARAÚJO, C. S. S. 700 1 $aLEITE, B. G. S. 700 1 $aROQUE, F. A. 700 1 $aARAÚJO, L. F. 773 $tPoultry Science$gv. 99, n. 11, 2020.
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Embrapa Instrumentação (CNPDIA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
06/07/1999 |
Data da última atualização: |
27/01/2015 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
OLIVEIRA, M. R. C. de; GAIA, J. M. D.; MARTINS, C. da S.; POLTRONIERI, M. C.; ALVES, R. M. |
Afiliação: |
MARIA ROSA C. DE OLIVEIRA, CPATU; JOSÉ MARIA D. GAIA, CPATU; CARLOS DA SILVA MARTINS, CPATU; MARLI COSTA POLTRONIERI, CPATU; RAFAEL MOYSES ALVES, CPATU. |
Título: |
Protocolos para analise de isoenzimas em pimenta do reino. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Genetics and Molecular Biology, v. 21, n. 3, p. 243, 1998. Suplement. |
Idioma: |
Português |
Notas: |
Programas e resumos do Congresso Nacional de Genética, 44., 1998, Águas Lindoia. |
Palavras-Chave: |
Eletroforese de isoenzima; Estrutura genética do germoplasma; Isoenzymes; Pimenta-do-reino. |
Thesagro: |
Marcador Genético; Piper Nigrum. |
Thesaurus NAL: |
genetic markers; genetics; germplasm. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00855nam a2200265 a 4500 001 1399887 005 2015-01-27 008 1998 bl uuuu u00u1 u #d 100 1 $aOLIVEIRA, M. R. C. de 245 $aProtocolos para analise de isoenzimas em pimenta do reino. 260 $aGenetics and Molecular Biology, v. 21, n. 3, p. 243, 1998. Suplement.$c1998 500 $aProgramas e resumos do Congresso Nacional de Genética, 44., 1998, Águas Lindoia. 650 $agenetic markers 650 $agenetics 650 $agermplasm 650 $aMarcador Genético 650 $aPiper Nigrum 653 $aEletroforese de isoenzima 653 $aEstrutura genética do germoplasma 653 $aIsoenzymes 653 $aPimenta-do-reino 700 1 $aGAIA, J. M. D. 700 1 $aMARTINS, C. da S. 700 1 $aPOLTRONIERI, M. C. 700 1 $aALVES, R. M.
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